Coexistence of Two Species of Leishmania in the Digestive Tract of the Vector Lutzomyia ovallesi

Barrios, Miguel; Rodríguez, Noris; Dm, Feliciangeli; Ulrich, Marian; Telles, Senobia; Me, Pinardi; Convit, J

doi:10.4269/ajtmh.1994.51.669

Cited by 21 publications

(13 citation statements)

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“…The gels were stained with silver nitrate and photographed. Dot blot hybridization was carried out as was previously described (Barrios et al 1994). Pre-hybridization and hybridization were performed overnight at 42 o C in 50% formamide containing buffer.…”

Section: Methodsmentioning

confidence: 99%

Cotton Rats (Sigmodon hispidus) and Black Rats (Rattus rattus) as Possible Reservoirs of Leishmania spp. in Lara State, Venezuela

Lima

Guglielmo

Rodríguez

et al. 2002

Mem. Inst. Oswaldo Cruz

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A total of 519 wild animals belonging to eleven species were collected during a two year study in a cutaneous leishmaniasis endemic area in Venezuela (La Matica, Lara State). The animals were captured in home-made Tomahawk-like traps baited with maize, bananas or other available local fruits, and parasites were isolated from 27 specimens. Two different species were found naturally infected with flagellates, i.e., cotton rats (Sigmodon hispidus) and black rats (Rattus rattus).Characterization of the parasites using PCR, kDNA restriction pattern and hybridization with species-specific probes revealed the presence of Leishmania (L.) mexicana in three of the black rats and Leishmania (V.) braziliensis in two others. The latter species was also identified in the single positive specimen of S. hispidus. The results suggested both species of animals as possible reservoirs of Leishmania sp.

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Section: Methodsmentioning

confidence: 99%

Cotton Rats (Sigmodon hispidus) and Black Rats (Rattus rattus) as Possible Reservoirs of Leishmania spp. in Lara State, Venezuela

Lima

Guglielmo

Rodríguez

et al. 2002

Mem. Inst. Oswaldo Cruz

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“…Lutzomyia ovallesi is the most widespread vector of CL below 800 m a.s.l. in Venezuela, and has been found infected in several foci: El Ingenio, Miranda State (north-central Venezuela) with Leishmania (Viannia) braziliensis and Leishmania (Leishmania) mexicana (Feliciangeli, 1991;Feliciangeli et al, 1994;Barrios et al, 1994); in Duaca, Lara State (west-central Venezuela) it was found harboring a hybrid Le. braziliensis-Le.…”

Section: Introductionmentioning

confidence: 99%

Isolation ofLeishmania (Viannia) braziliensisfromLutzomyia spinicrassa(species groupVerrucarum) Morales Osorno Mesa, Osorno & Hoyos 1969, in the Venezuelan Andean Region

Perruolo

Rodríguez²,

Feliciangeli

2006

Parasite

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Summary :Natural infection with Leishmania spp. in phlebotomine sandflies was searched for during a longitudinal study carried out from July 1997 to July 1998 in the village Catarnica, Municipality Independencia, Táchira State. This hamlet is an old endemic focus of cutaneous leishmaniasis in the Venezuelan Andean region, which lies close to the Colombian border at 1,300 m a.s.l., in an agricultural area mainly used for cultivating coffee. Phlebotomine sandflies were collected using Shannon traps placed in the peridomestic habitat from 19:00 to 21:00 hs. Males were stored in alcohol 70 % while females were kept in Nunc vials with 10 % DMSO and cryopreserved in liquid nitrogen for subsequent dissection and identification. The most abundant anthropophilic species was Lutzomyia spinicrassa with 3,032 males and 4,290 females (85.4 %). Among 1,633 (38 %) females of Lu. spinicrassa dissected, 26 (1.6 %) were infected with promastigotes, while no natural infection was found in 209 females of other species. The flagellates were identified as Leishmania braziliensis braziliensis using PCR with species specific primers derived from nuclear DNA and hybridization using species specific probe labelled with digoxigenin. This parasite had been previously isolated from patients with cutaneous leishmaniasis from the same area. These results show Lu. spinicrassa as a new proven vector of Leishmania braziliensis in the Andean region of Venezuela. Résumé

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“…Many other flagellates have been isolated from sand fly vectors and/or mammalian hosts in the GEC without being identified. Unidentified Leishmania strains were isolated in the Brazilian Amazon region from the viscera of two Dasypus novemcinctus (peri-pylarian species biologically and biochemically different from 202 The life cycles of these species of unidentified, unknown, or uncertain taxonomic position have not been described and additional epidemiologic investigations are needed.…”

Section: Leishmania (V) Lainsonimentioning

confidence: 99%

Ecology of the Leishmania Species in the Guianan Ecoregion Complex

Rotureau

2006

The American Journal of Tropical Medicine and Hygiene

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Abstract. In the Guianan ecoregion complex, at least seven Leishmania species belonging to both the L. (Leishmania) Saf' janova, 1982 and L. (Viannia) Lainson and Shaw, 1987 sub-genera are causative agents of American visceral leishmaniasis, mucocutaneous leishmaniasis, and cutaneous leishmaniases, including localized and diffuse forms. They are mostly sylvatic species subjugated to their own specific Lutzomyia sand fly vectors and to particular wild mammalian reservoir hosts of the dense rain forest. Originally implicated strictly in wild zoonoses and developing in forested ecotopes, Leishmania show a particular adaptive plasticity to face environmental modifications following deforestation and anthropization. This review presents the different pathogenic complexes of these parasites, emphasizes their implication in human diseases, and considers the influence of humans on their ecology.

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Coexistence of Two Species of Leishmania in the Digestive Tract of the Vector Lutzomyia ovallesi

Cited by 21 publications

References 0 publications

Cotton Rats (Sigmodon hispidus) and Black Rats (Rattus rattus) as Possible Reservoirs of Leishmania spp. in Lara State, Venezuela

Cotton Rats (Sigmodon hispidus) and Black Rats (Rattus rattus) as Possible Reservoirs of Leishmania spp. in Lara State, Venezuela

Isolation ofLeishmania (Viannia) braziliensisfromLutzomyia spinicrassa(species groupVerrucarum) Morales Osorno Mesa, Osorno & Hoyos 1969, in the Venezuelan Andean Region

Ecology of the Leishmania Species in the Guianan Ecoregion Complex

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