Coffee Improves Insulin-Stimulated Akt Phosphorylation in Liver and Skeletal Muscle in Diabetic KK-Ay Mice

Kobayashi, Misato; Matsuda, Yuji; Iwai, Hiroshi; Hiramitsu, Masanori; Inoue, Takashi; Katagiri, Takao; Yamashita, Yoko; Ashida, Hitoshi; Murai, Atsushi; Horio, Fumihiko

doi:10.3177/jnsv.58.408

Cited by 15 publications

(10 citation statements)

References 24 publications

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“…The mechanism of this effect is not yet clear but could be a result of improved glucose uptake by skeletal muscle or up-regulation of insulin sensitizing hormones such as adiponectin [17], [20]. Though irisin is secreted in response to exercise, another state of increased energy expenditure, we did not detect a statistically significant change in irisin levels after eight weeks of coffee consumption [8].…”

Section: Discussioncontrasting

confidence: 56%

“…One proposed mechanism to explain this association is that coffee may facilitate glucose uptake by skeletal muscle via increased translocation of the GLUT4 transporter to the plasma membrane [17]–[19]. Also, coffee consumption has been associated with increased levels of adiponectin, a hormone thought to have insulin-sensitizing properties [20]–[22].…”

Section: Introductionmentioning

confidence: 99%

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Circulating Irisin Levels Are Not Affected by Coffee Intake: A Randomized Controlled Trial

et al. 2014

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Irisin, secreted by skeletal muscle and possibly fat, is hypothesized to play an important role in modulating energy expenditure, obesity and metabolism. Coffee consumption also increases energy expenditure and leads to positive metabolic effects, but whether these effects are mediated by irisin remains unknown. The objective of this study was to determine the association between baseline irisin levels and the metabolic profile in humans and to investigate whether consumption of caffeinated coffee alters irisin levels. To this end, a secondary analysis was performed investigating irisin levels at baseline and after eight weeks in 32 healthy, overweight coffee drinkers who were randomized to consumption of 5 cups per day of instant caffeinated coffee, decaffeinated coffee, or water. Spearman correlation and analysis of covariance analyses were performed to identify possible associations. Irisin levels were positively correlated with waist circumference (r = 0.41, p = 0.02), fat mass (r = 0.44, p = 0.01) and CRP (r = 0.47, p = 0.007). Though there was a trend towards increased levels of irisin over time in the caffeinated coffee group (+1.8%) when compared to the placebo group (−4%) this did not reach statistical significance (p = 0.75 for the trend). This first randomized trial failed to reveal any effects of coffee consumption on irisin levels, but a larger trial, appropriately sized on the basis of data provided by this study, is needed to conclusively investigate such a relationship.Trial RegistrationClinicaltrials.gov NCT00305097

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Section: Discussioncontrasting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Circulating Irisin Levels Are Not Affected by Coffee Intake: A Randomized Controlled Trial

et al. 2014

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“…In C57BL/6J mice fed a high-fat diet, coffee ingestion also improved insulin sensitivity (4). Furthermore, we found that coffee increased the activation of Akt in insulin signaling in the liver and skeletal muscle (5). Insulin resistance and impaired insulin secretion are associated with the development of type 2 diabetes.…”

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confidence: 70%

Coffee Ingestion Suppresses Hyperglycemia in Streptozotocin-Induced Diabetic Mice

Kobayashi

Kurata

Hamana

et al. 2017

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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Summary Coffee consumption reduces the risk of type 2 diabetes in humans, but the mechanism remains unclear. In this study, we investigated the effect of coffee on pancreatic b-cells in the induction of diabetes by streptozotocin (STZ) treatment in mice. We examined the effect of coffee, caffeine, or decaffeinated coffee ingestion on STZ-induced hyperglycemia. After STZ injection in Exp. 1 and 2, serum glucose concentration and water intake in coffee ingestion (Coffee group) tended to be lowered or was significantly lowered compared to those in water ingestion (Water group) instead of coffee. In Exp. 1, the values for water intake and serum glucose concentration in caffeine ingestion (Caffeine group) were similar to those in the Water group. In Exp. 2, serum glucose concentrations in the decaffeinated coffee ingestion (Decaf group) tended to be lower than those in the Water group. Pancreatic insulin contents tended to be higher in the Coffee and Decaf groups than in the Water group (Exp. 1 and 2). In Exp. 3, subsequently, we showed that coffee ingestion also suppressed the deterioration of hyperglycemia in diabetic mice which had been already injected with STZ. This study showed that coffee ingestion prevented the development of STZ-induced diabetes and suppressed hyperglycemia in STZ-diabetic mice. Caffeine or decaffeinated coffee ingestion did not significantly suppress STZ-induced hyperglycemia. These results suggest that the combination of caffeine and other components of decaffeinated coffee are needed for the preventive effect on pancreatic b-cell destruction. Coffee ingestion may contribute to the maintenance of pancreatic insulin contents. Key Words streptozotocin, diabetes, hyperglycemia, coffee, caffeine In 2015, 415 million people worldwide were estimated to be affected by type 2 diabetes (1). The International Diabetes Federation estimates that this will rise to 642 million individuals by the year 2040, and warns that the prevalence of type 2 diabetes will increase. These increases are caused by changes in environmental factors such as quantity and quality of food and exercise, and therefore modification of environmental factors can contribute to the prevention of type 2 diabetes.In humans, many studies have revealed an association between coffee consumption and decreased risk of type 2 diabetes. A meta-analysis of 28 studies published between 1966 and 2013 showed that daily consumption of six cups of coffee was associated with a 33% lower risk of diabetes, and that coffee consumption dose-dependently reduced the risk of type 2 diabetes (2). In addition, the reduction in type 2 diabetes risk was observed with both, caffeinated and decaffeinated coffee. However, the mechanism of the anti-diabetic effect of coffee remains unclear.Previously we reported that coffee had an anti-diabetic effect in both spontaneous and diet-induced type 2 diabetes in animal models (3, 4). In KK-A y mice, which spontaneously develop type 2 diabetes, coffee ingestion suppressed hyperglycemia, hyperinsulinemia, hyperlipidemia...

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“…Giani and others proved that high‐fructose diet caused a significant reduction of insulin sensitivity and p85‐PI3K expression in the skeletal muscle of rats (Giani and others ). Kobayashi and others proved that the phosphorylation of serine in PKB could improve insulin sensitivity and glucose uptake in skeletal muscle of diabetic KKAy mice (Kobayashi and others ). In CHS‐treated mice, insulin‐induced phosphorylation of p‐85‐PI3K, Ser473‐PKB, and Thr308‐PKB was upregulated in comparison to those in HFSD mice.…”

Section: Discussionmentioning

confidence: 99%

Fucosylated Chondroitin Sulfate from Sea Cucumber Improves Insulin Sensitivity via Activation of PI3K/PKB Pathway

Chang

et al. 2014

Journal of Food Science

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This study was to investigate the effects of fucosylated chondroitin sulfate (CHS) from sea cucumber on insulin sensitivity in skeletal muscle of type 2 diabetic mice induced by a high-fat high-sucrose diet (HFSD). CHS supplementation for 19 wk significantly improved insulin sensitivity by 20%, and reduced blood glucose and insulin levels. Western blotting assay showed that CHS significantly increased insulin-stimulated glucose transporter 4 (GLUT4) translocation to 1.7-fold, phosphorylation of phosphoinositide 3-kinase (PI3K) at p85 to 5.0-fold, protein kinase B (PKB) at Ser473 to 1.5-fold, and Thr308 to 1.6-fold in skeletal muscle. However, PI3K, PKB, and GLUT4 total proteins expression were unchangeable. In addition, qRT-PCR analysis proved that the insulin signaling was activated by CHS treatment, showing the increased mRNA expressions of glucose uptake-related key genes. It indicated that CHS improved insulin sensitivity by activation of PI3K/PKB signaling in skeletal muscle of type 2 diabetic mice. Identification of potential mechanism by which CHS increased insulin sensitivity might provide a new functional food or pharmaceutical application of sea cucumber.

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Coffee Improves Insulin-Stimulated Akt Phosphorylation in Liver and Skeletal Muscle in Diabetic KK-Ay Mice

Abstract: Abbreviations: GLUT4, glucose transporter 4; IL-6, interleukin 6; IR, insulin receptor; IRS-1, insulin receptor substrate-1; MCP-1, monocyte chemoattractant protein-1; PI3K, phosphatidylinositol 3-kinase; TNF-a, tumor necrosis factor a.

Cited by 15 publications

References 24 publications

Circulating Irisin Levels Are Not Affected by Coffee Intake: A Randomized Controlled Trial

Circulating Irisin Levels Are Not Affected by Coffee Intake: A Randomized Controlled Trial

Coffee Ingestion Suppresses Hyperglycemia in Streptozotocin-Induced Diabetic Mice

Fucosylated Chondroitin Sulfate from Sea Cucumber Improves Insulin Sensitivity via Activation of PI3K/PKB Pathway

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