Coffee polyphenols suppress diet-induced body fat accumulation by downregulating SREBP-1c and related molecules in C57BL/6J mice

Abstract

The prevalence of obesity is increasing globally, and obesity is a major risk factor for type 2 diabetes and cardiovascular disease. We investigated the effects of coffee polyphenols (CPP), which are abundant in coffee and consumed worldwide, on diet-induced body fat accumulation. C57BL/6J mice were fed either a control diet, a high-fat diet, or a high-fat diet supplemented with 0.5 to 1.0% CPP for 2-15 wk. Supplementation with CPP significantly reduced body weight gain, abdominal and liver fat accumulation, and infiltration of macrophages into adipose tissues. Energy expenditure evaluated by indirect calorimetry was significantly increased in CPP-fed mice. The mRNA levels of sterol regulatory element-binding protein (SREBP)-1c, acetyl-CoA carboxylase-1 and -2, stearoyl-CoA desaturase-1, and pyruvate dehydrogenase kinase-4 in the liver were significantly lower in CPP-fed mice than in high-fat control mice. Similarly, CPP suppressed the expression of these molecules in Hepa 1-6 cells, concomitant with an increase in microRNA-122. Structure-activity relationship studies of nine quinic acid derivatives isolated from CPP in Hepa 1-6 cells suggested that mono- or di-caffeoyl quinic acids (CQA) are active substances in the beneficial effects of CPP. Furthermore, CPP and 5-CQA decreased the nuclear active form of SREBP-1, acetyl-CoA carboxylase activity, and cellular malonyl-CoA levels. These findings indicate that CPP enhances energy metabolism and reduces lipogenesis by downregulating SREBP-1c and related molecules, which leads to the suppression of body fat accumulation.

Stimulation of Postprandial Fat Utilization in Healthy Humans by Daily Consumption of Chlorogenic Acids

“…[22][23][24][25] Daily ingestion by rodents of nonflavonoid CGAs may mediate fat catabolism as well as green tea polyphenols (flavonoids). 26) However, the relationship between daily CGA ingestion and energy consumption in humans is not currently known.…”

Anti-obesity effect of robusta fermented with Leuconostoc mesenteroides in high-fat diet-induced obese mice

“…The findings in the present study indicated that both the 10% RMR-LM group and 20% RMR-LM group had significant ( * P<0.05) down-regulation of PPARγ mRNA expression in the liver. The expression of several lipogenic genes is regulated by SREBP-1 at the transcriptional level (21). Our evidence suggests that the 20% RMR-LM extract may decrease lipogenesis partly by regulating PPARγ signaling.…”

Green Coffee Bean Extract as a Weight Loss Supplement

“…Studies have shown that green coffee enhances energy metabolism and reduces lipogenesis by down regulating SREBP-1c (Sterol regulatory element-binding protein) and related molecules, which leads to the suppression of body fat accumulation [11]. Scientific theories expressed that natural substances found in green coffee prevent weight gain and fat accumulation by inhibiting fat absorption and increasing fat metabolism in liver [23,29].…”

“…Scientific theories expressed that natural substances found in green coffee prevent weight gain and fat accumulation by inhibiting fat absorption and increasing fat metabolism in liver [23,29]. Supplementation with coffee polyphenols can also be effective in weight loss and reduce abdominal and liver fat accumulation by inhibition of macrophages infiltration into adipose tissues [11].…”

“…Coffee is one of the most commonly consumed beverages in the world that its beneficial effects on human health have been a subject of many studies [8][9][10][11][12]. One of the common traditional forms of coffee is green coffee extract (GCE) that prepared from green or raw (unroasted) coffee been.…”