Cofilin-1 and phosphoglycerate kinase 1 as promising indicators for glioma radiosensibility and prognosis

Abstract

Glioma is a primary malignancy in central nervous system. Radiotherapy has been used as one of the standard treatments for glioma for decades. Since radioresistance can reduce the curative efficacy of radiotherapy in glioma, investigating the cause of radioresistance and predicting the tumour radiosensibility appeared particularly important. We previously reported that CFL1 and PGK1 are over-expressed in radioresistant U251 glioma cells. In this study, the level of CFL1 and PGK1 of 113 glioma tissues were measured by ELISA method. The relevance of the expression of these two proteins to radiosensibility was analyzed by mean test and multivariate logistic regression. The survival analysis was carried out in 85 irradiated patients and 105 followed-up patients respectively. The relationship between protein expression and clinical parameters was explored in overall 113 patients, and the correlation between CFL1 and PGK1 were determined as well. Our results showed that the expression of CFL1 and PGK1 were significantly higher (P < 0.001) in radioresistant patients than others. The multivariate Logistic regression demonstrated that the expression of CFL1 (p < 0.001) and PGK1 (p < 0.001) were associated with radioresistance in glioma. The multivariate Cox regression in overall survival suggested that CFL1 level or PGK1 level could be the independent prognosis factor for poor prognosis in 113 glioma patients. In addition, CFL1 expression was positively correlated with PGK1 expression in glioma. The results suggested that as promising indicators, CFL1 and PGK1 could be used to evaluate glioma radiosensibility and prognosis. These two proteins could also be the potential therapeutic targets of glioma.

Oral Mucosal Organoids as a Potential Platform for Personalized Cancer Therapy

“…S13; Supplementary Table S5). Although differentially expressed genes could be detected in all comparisons, including those previously described associated with resistance to these therapies (52)(53)(54)(55), no clear indication for resistance mechanisms could be deduced from performing gene enrichment analysis on these gene sets.…”

“…For example, genes enriched in radiotherapyresistant organoid lines included PGK1 and GPX3. For both genes, high expression has previously been associated with poor response to radiotherapy or DNA-damaging therapies (52,53). Expression of IGFBP5 and SLC16A3, both enriched in cetuximab-resistant organoid lines, have previously been reported to be biomarkers for poor response to cetuximab (54,55).…”

RETRACTED: The effect of CELLFOODTM on radiotherapy or combined chemoradiotherapy: preclinical evidence

“…61 Recently, the suppression of PGK1 was reported to enhance the radiosensitivity of U251 xenografts and that PGK1 with Cofilin1 could be used to evaluate glioma radiosensibility and prognosis. 62 In our mice with mesothelioma, the reduction of PGK1 expression after CF+RT(4) and ST + CF + RT(4) versus RT(4) treatment was observed, showing greater radiosensitivity in irradiated mice in association with CF treatment.…”

A Novel lncRNA&nbsp;ARST&nbsp;Represses Glioma Progression by Inhibiting ALDOA-mediated Actin Cytoskeleton Integrity

“…Compared with the cells in normal tissues, ALDOA and co lin were often overexpressed in the cells of various tumors. Especially in the glioma cells, which exhibited a strong invasive ability, co lin was signi cantly upregulated [31,32]. High level of co lin led to the enhanced depolymerization and repolymerization of actin bers which promoted malignant invasion and migration of gliomas [33].…”