Coiled fiber scaffolds embedded with gold nanoparticles improve the performance of engineered cardiac tissues

Fleischer, Sharon; Shevach, Michal; Feiner, Ron; Dvir, Tal

doi:10.1039/c4nr00300d

Cited by 134 publications

(106 citation statements)

References 27 publications

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“…Electrospinning is a well-established method used in tissue engineering to fabricate fibrous scaffolds (9,10,(22)(23)(24). We have recently reported on the potential of albumin fiber scaffolds to promote the assembly of anisotropic and functional cardiac patches.…”

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confidence: 99%

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Modular assembly of thick multifunctional cardiac patches

Fleischer

Shapira

Feiner

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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133

110

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In cardiac tissue engineering cells are seeded within porous biomaterial scaffolds to create functional cardiac patches. Here, we report on a bottom-up approach to assemble a modular tissue consisting of multiple layers with distinct structures and functions. Albumin electrospun fiber scaffolds were laser-patterned to create microgrooves for engineering aligned cardiac tissues exhibiting anisotropic electrical signal propagation. Microchannels were patterned within the scaffolds and seeded with endothelial cells to form closed lumens. Moreover, cage-like structures were patterned within the scaffolds and accommodated poly(lactic-co-glycolic acid) (PLGA) microparticulate systems that controlled the release of VEGF, which promotes vascularization, or dexamethasone, an anti-inflammatory agent. The structure, morphology, and function of each layer were characterized, and the tissue layers were grown separately in their optimal conditions. Before transplantation the tissue and microparticulate layers were integrated by an ECM-based biological glue to form thick 3D cardiac patches. Finally, the patches were transplanted in rats, and their vascularization was assessed. Because of the simple modularity of this approach, we believe that it could be used in the future to assemble other multicellular, thick, 3D, functional tissues.

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confidence: 99%

“…Numerous approaches have been used to recapitulate different structural properties of the heart (7)(8)(9)(10)(11). Technologies such as lithography and microfabrication enabled precise control over the biomaterial pattern and thus over the induced cell alignment and anisotropic electrical signal transfer (8,12).…”

mentioning

confidence: 99%

Modular assembly of thick multifunctional cardiac patches

Fleischer

Shapira

Feiner

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

133

110

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“…For example, gold nanowires were incorporated into porous alginate scaffolds to improve scaffold conductivity for cardiac cell culture (81). To mimic the coiled fibers of the native heart matrix as well as high electrical conductivity, neonatal rat CMs were cultivated on scaffolds generated from electrospun poly(ε-caprolactone) micro-fibers doped with gold nanoparticles (82). Tough, yet flexible scaffolds with enhanced electrical properties were created by incorporating carbon nanotubes (CNTs) into aligned poly(glycerol sebacate):gelatin electrospun nanofibers (83).…”

Section: Tissue Engineering and Biomaterials For The Restoration Omentioning

confidence: 99%

The Role of Tissue Engineering and Biomaterials in Cardiac Regenerative Medicine

Zhao

Feric

Thavandiran

et al. 2014

Canadian Journal of Cardiology

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In recent years, the development of three-dimensional engineered heart tissue (EHT) has made large strides forward due to advances in stem cell biology, materials science, pre-vascularization strategies and nanotechnology. As a result, the role of tissue engineering in cardiac regenerative medicine has become multi-faceted as new applications become feasible. Cardiac tissue engineering has long been established to have the potential to partially or fully restore cardiac function following cardiac injury. However, EHTs may also serve as surrogate human cardiac tissue for drug-related toxicity screening. Cardiotoxicity remains a major cause of drug withdrawal in the pharmaceutical industry. Unsafe drugs reach the market because pre-clinical evaluation is insufficient to weed out cardiotoxic drugs in all their forms. Bioengineering methods could provide functional and mature human myocardial tissues, i.e. physiologically relevant platforms, for screening the cardiotoxic effects of pharmaceutical agents and facilitate the discovery of new therapeutic agents. Finally, advances in induced pluripotent stem cells have made patient-specific EHTs possible, which opens up the possibility of personalized medicine. Herein, we give an overview of the present state of the art in cardiac tissue engineering, the challenges to the field and future perspectives.

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“…The incorporation of gold nanoparticles (AuNPs) is another conductive substance that is used to improve conductivity and mechanical properties. Fleischer et al added AuNPs to PCL and reported that this addition of AuNPs affected the morphology of the cardiac tissues [136]. The morphology of the cells was elongated and aligned, which mimics the morphology inside the myocardium.…”

Section: Co-electrospinning With Conductive Materialsmentioning

confidence: 99%

Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering

et al. 2017

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Please cite this article as: Kitsara, M., Agbulut, O., Kontziampasis, D., Chen, Y., Menasché, P., Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering, Acta Biomaterialia (2016), doi: http://dx.doi.org/ 10. 1016/j.actbio.2016.11.014 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractCardiac cell therapy holds a real promise for improving heart function and especially of the chronically failing myocardium. Embedding cells into 3D biodegradable scaffolds may better preserve cell survival and enhance cell engraftment after transplantation, consequently improving cardiac cell therapy compared with direct intramyocardial injection of isolated cells.The primary objective of a scaffold used in tissue engineering is the recreation of the natural 3D environment most suitable for an adequate tissue growth. An important aspect of this commitment is to mimic the fibrillar structure of the extracellular matrix, which provides essential guidance for cell organization, survival, and function. Recent advances in nanotechnology have significantly improved our capacities to mimic the extracellular matrix. Among them, electrospinning is well known for being easy to process and cost effective. Consequently, it is becoming increasingly popular for biomedical applications and it is most definitely the cutting edge technique to make scaffolds that mimic the extracellular matrix for industrial applications.Here, the desirable physico-chemical properties of the electrospun scaffolds for cardiac therapy are described, and polymers are categorized to natural and synthetic. Moreover, the methods used for improving functionalities by providing cells with the necessary chemical cues and a more in vivo-like environment are reported.

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Coiled fiber scaffolds embedded with gold nanoparticles improve the performance of engineered cardiac tissues

Cited by 134 publications

References 27 publications

Modular assembly of thick multifunctional cardiac patches

Modular assembly of thick multifunctional cardiac patches

The Role of Tissue Engineering and Biomaterials in Cardiac Regenerative Medicine

Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering

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