Colchicine induced asynaptic chromosomal behavior at meiosis in Allium cepa L.

Ahirwar, Ramesh; Verma, R. C.

doi:10.1007/s13237-015-0133-4

Cited by 7 publications

(9 citation statements)

References 20 publications

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“…1). Mitoinhibitory effects of gamma radiation at higher doses has been reported earlier in Hordeum vulgare (Eroglu et al, 2007), Allium cepa (Ahirwar, 2015) and Triticum aestivum (Borzouei et al, 2010). Stickiness (Fig.…”

Section: O N L I N E C O P ÿmentioning

confidence: 56%

Ionizing radiation mediated effect on morphological, biochemical and microsporogenesis behavior of Artemisia annua L.

Singh¹,

Kumar²

2020

JEB

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To evaluate the effect of gamma rays on the cytological, morphological and biochemical aspects of Artemisia annua L. 60 Fresh seeds of Artemisia annua L. were treated with gamma rays (100 Gy, 200 Gy, 300 Gy& 400 Gy) through a Co source. Along with one set of control, the seeds were sown in triplicates to raise population for meiotic study. Mature capitula were collected for pollen study. Mutagen sensitivity was also studied using morphological (germination percentage, survival percentage, plant height and internodal length) and biochemical (chlorophyll content) parameters.Total abnormality percentage (TAB%) showed direct relationship with the increasing doses of gamma rays and different chromosomal aberrations such as scattering, precocious, laggard and bridge formation etc. were also observed. Stickiness was the main chromosomal abnormality observed in treated sets, mostly at 300Gy and 400Gy doses. The results clearly elucidate that the higher doses of gamma rays substantially affected the growth parameters as evident from the data of morphological and biochemical parameter, which were considerably reduced with the increase in dose of gamma rays. At higher doses, some chlorophyll variants were observed like chlorine, xantha and viridis etc.Gamma rays had induced sufficient genetic variability in Artemisia, hence, induced cytological disturbance alters genetic material which can be inherited to the subsequent generation. These genetic variability creates agronomically superior mutants which will be promising materials for plant breeders in near future.

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Section: O N L I N E C O P ÿmentioning

confidence: 56%

Ionizing radiation mediated effect on morphological, biochemical and microsporogenesis behavior of Artemisia annua L.

Singh¹,

Kumar²

2020

JEB

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“…Although, colchicine has been normally utilized for genomic doubling in plants, studies unraveling its influence on meiosis are still lacking. Colchicine acts by binding to the tubulin dimers, preventing the formation of microtubules on the spindle fibers during cell division and, thus causing various meiotic irregularities including structural as well as numerical chromosomal alterations (Verma et al 1992, 1993, Ahirwar and Verma 2015. Detailed assessment on spectrum and frequency of chromosomal damage indicated that colchicine is a potent chemical mutagen in modifying the genomic architecture of T. durum.…”

Section: Discussionmentioning

confidence: 99%

“…Univalents may have been produced as a result of partial failure of chromosome pairing among homologous chromosomes at prometaphase-I, due to the phenomenon called desynapsis (Verma and Khah 2016). Colchicineinduced univalent chromosome behavior was also observed by Ahirwar and Verma (2015) in Allium cepa plants.…”

Section: Discussionmentioning

confidence: 99%

“…As colchicine causes spindle dysfunction, this results in the production of unoriented or stray bivalents and precocious chromosome movement at metaphase I, laggards, disturbed polarity, and chromatin bridges at anaphase and telophase I/II. Early chiasma terminalization creates precocious movement of chromosomes whereas late chiasma terminalization causes bridge formation at anaphase I. Univalents and multivalents often lead to the formation of laggard chromosomes at anaphase and telophase stages (Ahirwar andVerma 2015, Khah andVerma 2017b). The occurrence of laggard chromosomes and acentric fragments in cells may also be due to chromosomal breakage resulting from disturbances to DNA at GC-rich regions (Bhat et al 2007).…”

Section: Discussionmentioning

confidence: 99%

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Influence of Colchicine in Causing Severe Chromosomal Damage in Microsporocytes of Hard Wheat (<i>Triticum durum</i> Desf.): Possible Mechanisms and Genotoxic Relevance

et al. 2022

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In this study, cytogenetic toxicity of colchicine in microsporocytes of hard wheat (Triticum durum Desf.) was evaluated. Colchicine treatments revealed the induction of various chromosomal abnormalities in gametic cells which includes chromatin stickiness, unoriented chromosomes, univalents, laggards, bridges, and micronuclei. These abnormalities increased along with increased colchicine concentrations. Simultaneously, the study also deals correlation of overall cytological aberrations with the frequency of seed germination, plant survival, and pollen fertility. Chromosome stickiness along with spindle disturbances, laggards, chromatin bridges, irregular chromosome distribution, and abnormal tetrads are the main causes that lead to a reduction in pollen fertility percentage found in colchicine-treated populations. Furthermore, the colchicine-induced chromosome aberrations caused a significant decline in seed germination and plant survival with the increasing colchicine concentrations. The present findings reveal the potential of colchicine in inducing alterations in chromosome structure and behavior thereby enhancing the genetic diversity in hard wheat. As a result, colchicine could be efficiently exploited for isolating novel and promising mutants in segregating generations.

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“…Previous works aimed at mitotic inception are in Chrysanthemum carinatum (Verma and Chandel 1994), Allium cepa L. (Ahirwar 2013) and Crotalaria juncea (Kumar and Dwivedi 2014). Singh and Datta (2010) have recently shown that low dose of gamma irradiation could be potentially exploited for improving plant vigor and grain productivity.…”

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confidence: 99%

Germination and cytological aspects of <i>Dolichos lablab</i> L. exposed to gamma radiation

Kumar

Keserwani

2017

Chromosome Botany

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ABSTRACT:The present study aims to determine the impact of gamma radiation on the germination % and mitotic indices in three varieties of Dolichos lablab L. viz. Arkajay, Swarna uthkrishit and RP-08-31, at different doses of gamma rays i.e. 100, 200, 300, 400 and 500 Gy. Through the acquisition of the following experiment, an attempt to screen out the superior variety among the three selected varieties was done for Dolichos lablab. Among the three varieties studied, Arkajay showed the highest germination % and active mitotic index (AMI). A significant decline in AMI and increment in total abnormality percentage (TAB%) was observed with the increase in gamma rays from lower to higher doses. TAB% was induced due to the ionizing radiation. Stickiness, precocious movement, laggard and unorientation were found as preponderant chromosomal abnormalies. However, stickiness was the major abnormality at both metaphase and anaphase stages of cell division. In the present study, variety Arkajay emerged as the best variety based on the fact that it showed highest germination percentage and AMI among all of the three varieties selected.

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Colchicine induced asynaptic chromosomal behavior at meiosis in Allium cepa L.

Cited by 7 publications

References 20 publications

Ionizing radiation mediated effect on morphological, biochemical and microsporogenesis behavior of Artemisia annua L.

Ionizing radiation mediated effect on morphological, biochemical and microsporogenesis behavior of Artemisia annua L.

Influence of Colchicine in Causing Severe Chromosomal Damage in Microsporocytes of Hard Wheat (<i>Triticum durum</i> Desf.): Possible Mechanisms and Genotoxic Relevance

Germination and cytological aspects of <i>Dolichos lablab</i> L. exposed to gamma radiation

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