2014
DOI: 10.1242/bio.20147708
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Colchicine-induced degeneration of the micronucleus during conjugation in Tetrahymena

Abstract: One of the most dramatic examples of nuclear morphogenesis occurs during conjugation in Tetrahymena when the micronucleus elongates to a size longer than the cell itself. After contraction to a spherical shape, the nucleus moves directly to chromosome separation in the first meiotic division. Here we investigate the consequences of interrupting the elongation process. Colchicine, a microtubule inhibitor, caused retraction of elongated structures. With time, cells began to lose their micronuclei, and by five ho… Show more

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Cited by 2 publications
(2 citation statements)
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“…The 3D cell culture was stained with Hoechst 33342 (ThermoFisher, Waltham, MA, USA), Acridine Orange (ThermoFisher, Waltham, MA, USA) and Propidium Iodide (Sigma Aldrich, Saint Louis, MO, USA) solutions in order to dye the nucleus, identify alive and apoptotic cells, identify the necrotic cells, respectively. The nucleus of the cell is in blue due to Hoechst 33342 (5 µg/mL in PBS) [46,47]. Propidium Iodide (100 µg/mL in deionized water) dies selectively in red the nucleus of necrotic cells and is discarded by alive or apoptotic cells [48,49,50], while Acridine Orange (10 µg/mL in deionized water) dies the cell cytoplasm and nucleus in green if the cells are alive [46,47,51].…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The 3D cell culture was stained with Hoechst 33342 (ThermoFisher, Waltham, MA, USA), Acridine Orange (ThermoFisher, Waltham, MA, USA) and Propidium Iodide (Sigma Aldrich, Saint Louis, MO, USA) solutions in order to dye the nucleus, identify alive and apoptotic cells, identify the necrotic cells, respectively. The nucleus of the cell is in blue due to Hoechst 33342 (5 µg/mL in PBS) [46,47]. Propidium Iodide (100 µg/mL in deionized water) dies selectively in red the nucleus of necrotic cells and is discarded by alive or apoptotic cells [48,49,50], while Acridine Orange (10 µg/mL in deionized water) dies the cell cytoplasm and nucleus in green if the cells are alive [46,47,51].…”
Section: Methodsmentioning
confidence: 99%
“…The nucleus of the cell is in blue due to Hoechst 33342 (5 µg/mL in PBS) [46,47]. Propidium Iodide (100 µg/mL in deionized water) dies selectively in red the nucleus of necrotic cells and is discarded by alive or apoptotic cells [48,49,50], while Acridine Orange (10 µg/mL in deionized water) dies the cell cytoplasm and nucleus in green if the cells are alive [46,47,51]. In acridine orange staining, apoptotic cells are characterized by disaggregated DNA, while alive cells possess a well distinguishable nucleus [52,53,54,55].…”
Section: Methodsmentioning
confidence: 99%