Collagen-Derived Membrane: Corneal Implantation

Dunn, Max S.; Nishihara, Tomio; Stenzel, Kurt H.; Branwood, A. W.; Rubin, Albert L.

doi:10.1007/978-1-4615-6555-0_15

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…Collagen has previously been used by other groups for uses in the cornea, such as intracorneal lamellar implants, 7 and as contact lens for wound healing and drug delivery. 8 We recently reported the development of a corneal substitute that is made up of a matrix of cross-linked type I collagen that promoted corneal cell and nerve repair over 6 months.…”

mentioning

confidence: 99%

Regeneration of Corneal Cells and Nerves in an Implanted Collagen Corneal Substitute

McLaughlin¹,

Muzakare²,

Lagali³

et al. 2008

Cornea

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mentioning

confidence: 99%

Regeneration of Corneal Cells and Nerves in an Implanted Collagen Corneal Substitute

McLaughlin¹,

Muzakare²,

Lagali³

et al. 2008

Cornea

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“…In contrast to early experiments where membranes containing collagen from calfskin showed almost no invasion of keratocytes or any evidence of degradation, migration of keratocytes into the collagenous matrix and a concomitant remodeling of the collagen by the degradation with de novo production of collagen fibers was seen in the biomaterials used in our study after intrastromal placement. 34 Even though the collagen membranes used here were not optical clear from the beginning transparency gradually increased over time after intrastromal implantation presumably due to the remodeling of the hosted collagen fibers, which was seen histologically. However, cross-linked variants showed characteristics similar to those of amniotic membrane with much less signs of degradation after intrastromal implantation.…”

Section: Fig 8 (A)mentioning

confidence: 95%

Novel Collagen Membranes for the Reconstruction of the Corneal Surface

Petsch

Schlötzer‐Schrehardt²,

Meyer‐Blazejewska³

et al. 2014

Tissue Engineering Part A

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No standardized biomaterial exists for the surgical treatment of persistent corneal erosions and ulcerations. We analyzed the suitability and biocompatibility of defined noncross-linked and UV/riboflavin cross-linked equine type I collagen membranes for the reconstruction of the corneal surface. Isolated human oral mucosa epithelial cells, a cell type in clinical use for the treatment of ocular surface diseases, were subcultivated on both types of membranes and examined concerning cell adhesion, proliferation, and differentiation. Biocompatibility was evaluated following superficial and intrastromal corneal transplantation in New Zealand white rabbits. In cell cultures all collagen membranes supported adhesion of oral mucosa epithelial cells leading to the formation of multilayered epithelial cell sheets. After intrastromal corneal implantation clinical signs of degradation were seen in all variants of collagen membranes, which was fastest in noncross-linked variants. The histological and ultrastructural level invasion of keratocytes and production of new collagen fibers inside the collagen membranes could be detected in noncross-linked variants. After superficial corneal implantation covering of the membranes by corneal epithelium over time was visible. Ultrastructural analysis showed a slower rate of degradation and less invading keratocytes in cross-linked variants compared with noncross-linked collagen membranes. Cross-linked and noncross-linked variants of the collagen membrane proofed to be suitable to serve as a carrier for epithelial stem cells in vitro and showed a high biocompatibility in vivo. These results indicate that the tested collagen membranes might be suitable for the reconstruction of the corneal surface in patients with nonhealing ulcerations. Whether membranes with faster or slower degradation properties are preferable for the treatment of persistent corneal ulcerations might depend on the underlying corneal pathology and the degree of concomitant inflammation.

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Collagen-Derived Membrane: Corneal Implantation

Cited by 2 publications

References 7 publications

Regeneration of Corneal Cells and Nerves in an Implanted Collagen Corneal Substitute

Regeneration of Corneal Cells and Nerves in an Implanted Collagen Corneal Substitute

Novel Collagen Membranes for the Reconstruction of the Corneal Surface

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