Collagen gel systems for sustained delivery and tissue engineering

Wallace, Donald G.

doi:10.1016/j.addr.2003.08.004

Cited by 462 publications

(358 citation statements)

References 61 publications

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“…Gels, such as collagen type I [3,4] and platelet-rich plasma [5], have been investigated but their use is currently limited due to issues with purity and regulatory standards (such as the FDA and MHRA), and their inherent weak mechanical properties that are essential for this type of tissue. Electrospun fibre scaffolds are finding increasing use…”

Section: Introductionmentioning

confidence: 99%

Travelling along the Clinical Roadmap: Developing Electrospun Scaffolds for Tendon Repair

Bosworth

2014

Conference Papers in Science

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Biopolymers, such as poly( -caprolactone), can be easily electrospun to create fibrous scaffolds. It is also possible to control the alignment of the emitted fibres and further manipulate these scaffolds to create 3D yarn structures, which resemble part of the tendon tissue hierarchy. Material properties, such as tensile strength, can be tailored depending on the selection and combination of polymer and solvent used during electrospinning. The scaffolds have been proven to separately support the adhesion and proliferation of equine tendon fibroblasts and human mesenchymal stem cells whilst simultaneously directing cell orientation, which caused their alignment parallel to the underlying fibres. Implantation of scaffolds into the flexor digitorum profundus tendon of mice hindpaws yielded encouraging results with minimal inflammatory reaction and observation of cell infiltration into the scaffold. This research demonstrates the progression of electrospun fibres along the clinical roadmap towards becoming a future medical device for the treatment of tendon injuries.

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Section: Introductionmentioning

confidence: 99%

Travelling along the Clinical Roadmap: Developing Electrospun Scaffolds for Tendon Repair

Bosworth

2014

Conference Papers in Science

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“…SIS suspensions are not covalent and not cross-linked. Alternatively, SIS in an injected tissue body can have viscous properties through entanglements through hydrophobic and electrostatic interactions of collagenous fibers (Wallace and Rosenblatt, 2003). This behavior is advantageous for injectable delivery, since the SIS suspension can be easily fluidized at room temperature, but become relatively rigid at body temperature.…”

Section: Discussionmentioning

confidence: 99%

“…Various injectable drug depots, like those using collagen, gelatin, hyaluronate, cellulose, and fibrin, as well as synthetic materials, have been developed for this purpose (Kim et al, 2011;Kang et al, 2010;Lee et al, 2010;Yang et al, 2009;Yu and Ding, 2008;Lupton and Alster, 2000). Among them, collagen has the advantage of mimicking many features of the extracellular matrix (ECM) of mammals and it has high mechanical strength, good biocompatibility, low antigenicity, and water-uptake properties (Bhang et al, 2009;Kim et al, 2009;Ruozi et al, 2009;Pollack, 1999;Wallace and Rosenblatt, 2003). In addition, collagens easily flow in biological mediums, suggesting the possibility of it acting as an easily injectable drug depot (Wallace and Rosenblatt, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Among them, collagen has the advantage of mimicking many features of the extracellular matrix (ECM) of mammals and it has high mechanical strength, good biocompatibility, low antigenicity, and water-uptake properties (Bhang et al, 2009;Kim et al, 2009;Ruozi et al, 2009;Pollack, 1999;Wallace and Rosenblatt, 2003). In addition, collagens easily flow in biological mediums, suggesting the possibility of it acting as an easily injectable drug depot (Wallace and Rosenblatt, 2003).…”

Section: Introductionmentioning

confidence: 99%

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In vivo release of bovine serum albumin from an injectable small intestinal submucosa gel

Kang

Kim

Yoon

et al. 2011

International Journal of Pharmaceutics

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“…TGF-β3 is a type of protein produced by almost all types of cells (Schwartz et al, 1993) ant it is soluble in an acidic environment as well as water resulting in a sticky jelly (Wallace and Rosenblatt, 2003) and, thus, it is not appropriate to dissolve it in water and instead aliquot in an micro-litre tube with the final solution less than 1ml.…”

Section: Solventmentioning

confidence: 99%

Effect of transforming growth factor-β3 on mono and multilayer chondrocytes

et al. 2016

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Articular cartilage is an avascular and flexible connective tissue found in joints. It produces a cushioning effect at the joints and provides low friction to protect the ends of the bones from wear and tear/damage. It has poor repair capacity and any injury can result pain and loss of mobility. Transforming growth factor-beta (TGF-β), a cytokine superfamily, regulates cell function, including differentiation and proliferation. Although the function of the TGFβs in various cell types has been investigated, their function in cartilage repair is as yet not fully understood. The effect of TGF-β3 in biological regulation of primary chondrocyte was investigated in this work. TGF-β3 provide fibroblastic morphology to chondrocytes and therefore overall reduction in cell proliferation was observed. The length of the cells supplemented with TGF-β3 were larger than the cells without TGF-β3 treatment. This was caused by the fibroblast like cells (dedifferentiated chondrocytes) which occupied larger areas compared to cells without TGF-β3 addition. The healing process of the model wound closure assay of chondrocyte multilayer was slowed down by TGF-β3, and this cytokine negatively affected the strength of chondrocyte adhesion to the cell culture surface.

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Collagen gel systems for sustained delivery and tissue engineering

Cited by 462 publications

References 61 publications

Travelling along the Clinical Roadmap: Developing Electrospun Scaffolds for Tendon Repair

Travelling along the Clinical Roadmap: Developing Electrospun Scaffolds for Tendon Repair

In vivo release of bovine serum albumin from an injectable small intestinal submucosa gel

Effect of transforming growth factor-β3 on mono and multilayer chondrocytes

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