Collagen-nanoparticle Interactions: Type I Collagen Stabilization Using Functionalized Nanoparticles

Abstract

Collagen can absorb and associate with nanoparticles, which can have significant impact on the biological behavior of collagen and nanoparticles in a biological fluid. Iron oxide nanoparticles encapsulated with polystyrene-block-poly(acrylic acid) has been synthesized and characterized. Viscosity, circular dichroism, and Fourier transform infrared spectroscopy techniques have been used to study the Fe 2 O 3 -PS-b-PAA-collagen interaction. Encapsulated nanoparticles do not alter the collagen conformational stability and changes in functional groups are due to the aggregation of protein onto the surface of nanoparticle. The ability of PS-b-PAA encapsulated nanoparticles to maintain the collagen properties will have impact in the biomedical applications of the same.

Collagen Nanoparticles in Drug Delivery Systems and Tissue Engineering

“…A number of studies have reported that collagen-based wound dressings engineered via self-assembly have been demonstrated to promote fibroblast production and accelerate wound healing [53]. The controlled release of biomolecules in collagen-based bioactive wound dressings can be achieved by varying the self-assembly conditions of the collagen constructs [27,54]. Vedanayagam et al prepared varied sizes of silver NPs (AgNPs)-10 nm, 35 nm, and 55 nm-using nutraceuticals such as Pectin as stabilization and reducing agents through the method of microwave irradiation.…”

“…Hard to control NP size, shape and the potential of protein strain exists [27,54] Desolvation/Simple Coacervation Increased encapsulation efficiency, Size and shape of NPs can be controlled using reaction conditions.…”

“…Current PNPs systems are sensitive to stimuli such as temperature, light, pH, oxidizing/reducing agents, magnetic fields and enzymes which increases efficiency and specificity for TERM applications [21] king of collagen with NPs results in stabilization of the nanoparticles and helps with entrapment of the drug, to attain steady and regulated drug release for ideal therapeutic reactions. Collagen associates and absorbs NPs, leading to significant impacts on their biological functioning in any biofluid [27,28]. Collagen NPs are advantageous over other natural and synthetic polymeric NPs as they have favourable biocompatibility and biodegradability, low antigenicity, high contact surface, reduced toxicity and high cationic-charge density potential as they possess many amino groups; in the absence of surface modification with target compounds, they have a restricted capacity to cross the blood brain barrier.…”

The Vitamin A and D Exposure of Cells Affects the Intracellular Uptake of Aluminum Nanomaterials and Its Agglomeration Behavior: A Chemo-Analytic Investigation

“…This process might be due to the increased collagen synthesis and reduced matrix metalloproteinase expression which is known to occur because of vitamin A treatment [16]. The resulting collagen increase facilitates NM collagen interactions [17] which might hinder the NM allocation. Upon co-application of vitamin D3 the ToF-SIMS analyses revealed a different cellular deposition pattern of Al 0 NMs when compared to that after co-exposure to retinol.…”

Preparation and characterization of novel anti-inflammatory biological agents based on piroxicam-loaded poly-ε-caprolactone nano-particles for sustained NSAID delivery

“…Also, the mild and long-lasting PCL degradability is another good feature that makes it a suitable vehicle for sustained delivery of PX (Manoukian et al, 2018). On the other hand, the use of collagen that is able to absorb, associate and interact with NPs, has a great impact on their biological behavior (Kandamachira et al, 2015). Collagen is one of the most abundant components of the extracellular matrix, which has unique biological properties and results in excellent interaction with cells (Rahmani Del Saghati et al, 2018;Del Bakhshayesh et al, 2019;Kayal et al, 2019;Taghipour et al, 2019).…”