Colloidal gold probe-based immunochromatographic assay for the rapid detection of brevetoxins in fishery product samples

Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed

“…The morphological character of antibody-labeled SeNPs was identified with that of colloidal gold particles conjugated by antibodies under TEM in previous work. 39 sensitivity of Mel test strips in liquid milk, milk powder, and animal feed Preliminary concentrations of MEL in liquid milk, milk powder, and animal feed were analyzed by LC-MS/MS, and the results showed that they contained MEL at levels of 11.4 µg/kg, 23.6 µg/kg, and 1.15 µg/kg, respectively. Liquid milk, milk powder, and animal feed were spiked with different concentrations of standard MEL, which was then detected by the MEL test strip.…”

Microbiological food safety in Malaysia from the academician’s perspective

“…Besides that, Niu et al (2014) detected S. aureus while Xu et al (2013) detected C. jejuni. Detection of toxins such as brevetoxins and staphylococcal enterotoxin B was also possible with lateral flow immunoassay (Zhou et al, 2009;Rong-Hwa et al, 2010). Recently, Zhao et al (2016) developed a ten-channel up-converting phosphor technology-based lateral flow (TC-UPT-LF) to perform ten simultaneous detections within 20 minutes.…”

Aflatoxin Measurement and Analysis

“…requiring only a sample extraction step before use; 2. simplicity of procedure with single step, e.g., only adding test solution to the sample pad on the strip; 3. rapid on-site detection within a few minutes (5-15 min); 4. concentration levels of target analytes can be observed directly with the naked eyes; 5. user-friendly format no need for skill personnel; 6. less interference due to chromatographic separation; and 7. low cost Because of these advantages, lateral flow strip assay has become one of the commercial and widely-used immunoassays for rapid determination of mycotoxins, such as ochratoxin A (Lai et al, 2009;Liu, Tsao, Wang, & Yu, 2008;Wang, Liu, Xu, Zhang, & Wang, 2007;Cho et al, 2005), deoxynivalenol (Kolosova, De Saeger, Sibanda, Verheijen, & Van Peteghem, 2007;Xu et al, 2010;Kolosova et al, 2008), T-2 Toxin (Molinelli et al, 2008), zearalenone (Kolosova, De Saeger, Sibanda, Verheijen, & Van Peteghem, 2007), fumonisin B1 (Wang, Quan, Lee, & Kennedy, 2006), aflatoxins (Sun, Zhao, Tang, Zhou, & Chu, 2005;Sheibani, Tabrizchi, & Ghaziaskar, 2008) and so on. The visual detection limit (VDL), defined as the minimum concentration producing the color on the test line significantly different or weaker to that on the test line of negative control strip without aflatoxin (Li, Wei, Yang, Li, & Deng, 2009;Zhou et al, 2009), was used to express the sensitivity of the lateral flow strip assay. The visual detection limit of published conventional lateral flow strip assay for aflatoxins are summaried in Table 2.…”