Colorimetric bio-barcode immunoassay for parathion based on amplification by using platinum nanoparticles acting as a nanozyme

Ge, Chunhua; Jin, Maojun; Yan, Mengmeng; Cui, Xueyan; Wang, Yuanshang; Zheng, Weijia; Qin, Guoxin; Zhang, Yudan; Li, Mingjie; Liao, Yun; Zhang, Xiuyuan; Yan, Feiyan; El‐Aty, A.M. Abd; Hacımüftüoğlu, Ahmet; Wang, Jing

doi:10.1007/s00604-019-3433-6

Cited by 31 publications

(15 citation statements)

References 33 publications

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“…Gold nanoparticles (AuNPs) were prepared according to the methods used in previous work, as described in the Supporting Information. The ssDNA and mAb were modified on the filtered AuNPs according to a previous report .…”

Section: Methodsmentioning

confidence: 99%

“…The MNP probes were prepared with OVA-hapten parathion as described in a previous report. 23 Briefly, 500 μL of EDC (10 mg•mL −1 ) and 500 μL of NHS (10 mg•mL −1 ) were added to 50 μL of carboxyl-functionalized MNPs (10 mg•mL −1 ) for 30 min in order to activate the carboxyl group of MNPs and then washed three times with MES (15 mM, pH 6.0). Subsequently, the supernatant was removed in the tube under a magnetic field.…”

Section: Methodsmentioning

confidence: 99%

“…20−22 However, the "sandwich model" assay designed by Mirkin and co-workers 12,13 is unsuitable for small molecules, such as pesticides. 23 In efforts made to remedy the drawback, our group first established a bio-barcode immunoassay for the analysis of small molecules (pesticides) based on oligonucleotide-modified AuNPs and ovalbumin (OVA)-hapten-labeled MNPs. This bio-barcode immunoassay has been successfully applied in fruits and vegetables to detect traces of triazophos.…”

Section: Introductionmentioning

confidence: 99%

“…To date, nanozymes have attracted much more attention compared to natural enzymes because of their simple preparation, low cost, catalytic properties, and high stability against environmental change . Among various inorganic nanozymes, platinum (Pt) nanozymes with good water solubility and excellent biological compatibility were labeled for a bio-barcode immunoassay for analysis. ,, However, the Pt nanomaterial has low stability because of its easy aggregation. Meanwhile, recent reports have shown that platinum-based bimetallic nanocatalysts, typically gold@platinum (Au@Pt) nanoparticles, were more active for a variety of electrochemical reactions .…”

Section: Introductionmentioning

confidence: 99%

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Competitive Bio-Barcode Immunoassay for Highly Sensitive Detection of Parathion Based on Bimetallic Nanozyme Catalysis

Ge¹,

Jin²,

Ma³

et al. 2019

J. Agric. Food Chem.

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A competitive sensitive bio-barcode immunoassay based on bimetallic nanozyme (Au@Pt: gold@platinum) catalysis has been designed for the detection of the pesticide parathion. Gold nanoparticles (AuNPs) were modified with single-stranded thiol oligonucleotides (ssDNAs) and monoclonal antibodies (mAbs) to form AuNP probes; magnetic nanoparticles (MNPs) were coated with ovalbumin (OVA)–parathion haptens as MNP probes, and bimetallic nanozyme (Au@Pt) nanoparticles functionalized with the complementary thiolated ssDNA were used as Au@Pt probes. The Au@Pt probes reacted with the AuNP probes through complementary base pairing. Further, parathion competed with MNP probes to bind the mAbs on the AuNP probes. Finally, the complex system was separated by a magnetic field. The released Au@Pt probes catalyzed a chromogenic system consisting of teramethylbenzidine (TMB). The bimetallic nanozyme-based bio-barcode immunoassay was performed on rice, pear, apple, and cabbage samples to verify the feasibility of the method. The immunoassay exhibited a linear response from 0.01 to 40 μg·kg–1, and the limit of detection (LOD) was 2.13 × 10–3 μg·kg–1. The recoveries and relative standard deviations (RSDs) ranged from 73.12 to 116.29% and 5.59 to 10.87%, respectively. The method was found to correlate well with data obtained by liquid chromatography–tandem mass spectrometry (LC–MS/MS). In conclusion, this method exhibits potential as a sensitive alternative method for the detection of a variety of pesticides, ensuring the safety of fruits and vegetables in agriculture.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Competitive Bio-Barcode Immunoassay for Highly Sensitive Detection of Parathion Based on Bimetallic Nanozyme Catalysis

Ge¹,

Jin²,

Ma³

et al. 2019

J. Agric. Food Chem.

Self Cite

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“…Besides, nanozymes are supposed to be more stable and have a lower cost. Therefore, more and more NLISA are being fabricated for the quantification of pesticides [111][112][113][114][115].…”

Section: Nanozyme-based Immunosensorsmentioning

confidence: 99%

Nanozyme-Participated Biosensing of Pesticides and Cholinesterases: A Critical Review

Zhu

Liu

et al. 2021

Biosensors

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To improve the output and quality of agricultural products, pesticides are globally utilized as an efficient tool to protect crops from insects. However, given that most pesticides used are difficult to decompose, they inevitably remain in agricultural products and are further enriched into food chains and ecosystems, posing great threats to human health and the environment. Thus, developing efficient methods and tools to monitor pesticide residues and related biomarkers (acetylcholinesterase and butylcholinesterase) became quite significant. With the advantages of excellent stability, tailorable catalytic performance, low cost, and easy mass production, nanomaterials with enzyme-like properties (nanozymes) are extensively utilized in fields ranging from biomedicine to environmental remediation. Especially, with the catalytic nature to offer amplified signals for highly sensitive detection, nanozymes were finding potential applications in the sensing of various analytes, including pesticides and their biomarkers. To highlight the progress in this field, here the sensing principles of pesticides and cholinesterases based on nanozyme catalysis are definitively summarized, and emerging detection methods and technologies with the participation of nanozymes are critically discussed. Importantly, typical examples are introduced to reveal the promising use of nanozymes. Also, some challenges in the field and future trends are proposed, with the hope of inspiring more efforts to advance nanozyme-involved sensors for pesticides and cholinesterases.

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Nanozyme-Based Sensors for Pesticide Detection

Prasad

Bansal

Ramanathan

2021

Environmental Chemistry for a Sustainable World

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Colorimetric bio-barcode immunoassay for parathion based on amplification by using platinum nanoparticles acting as a nanozyme

Cited by 31 publications

References 33 publications

Competitive Bio-Barcode Immunoassay for Highly Sensitive Detection of Parathion Based on Bimetallic Nanozyme Catalysis

Competitive Bio-Barcode Immunoassay for Highly Sensitive Detection of Parathion Based on Bimetallic Nanozyme Catalysis

Nanozyme-Participated Biosensing of Pesticides and Cholinesterases: A Critical Review

Nanozyme-Based Sensors for Pesticide Detection

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