Colorimetric determination of hexuronic acids in plant materials

Scott, Ralph W.

doi:10.1021/ac50043a036

Cited by 546 publications

(264 citation statements)

References 28 publications

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“…a-DGlucuronosyl-xylotriose was identified by TLC and HPLC using mGlcAX3 purchased from Megazyme as a standard. Furthermore, the putative mGlcAX3 was shown to contain hexuronic acid with the colorimetric reagent 3,5-dimethylphenol (Scott, 1979).…”

Section: Determination Of A-d-glucuronidase Activitymentioning

confidence: 99%

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-D-Glucuronidases from the xylanolytic thermophiles Clostridium stercorarium and Thermoanaerobacterium saccharolyticum

Bronnenmeier

Meissner²,

Stöcker³

et al. 1995

Microbiology

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a-D-Glucuronidases were purified from the xylanolytic thermophiles CIostridium stercorarium and Thermoanaerobacterium saccharolyticum. This enzyme activity was found to be intracellular in each organism, with T. saccharolyticum producing much greater total activity. The specific activities of the purified enzymes (10 U mg-1 T. saccharolyticum; 1.7 U mg-I C. stercorarium) differed by a factor of approximately 5. For the determination of enzyme activities, 4-Olmethyl-a-D-glucuronosyl-xylotriose was used as a substrate and the glucuronic acid released by a-D-glucuronidase action was quantified by a colorimetric procedure. &OlMethyl-a=D-glucuronosyl-xylotriose was the hydrolysis product that accumulated after exhaustive degradation of &O-methyl-a-D-gIucuronoxylan with xylanases of C. stercorarium. Hydrolysis of side chains in high-molecular-mass glucuronoxylan could not be detected. Neither of the enzymes was able to hydrolyse the chromogenic aryl-substrate p-n i trophenyl-a-D-g I ucuronoside. Both a-D-gl ucuron idases have a dimer ic structure, with monomeric molecular masses of 72 and 76 kDa for C. stercorarium and of 71 kDa for T. saccharolyticum. The pl was estimated to be 4.3 for each enzyme. While both enzymes exhibited a similar pH optimum (pH 556.5) they differed in their thermostabilities. A t 60 "C, half-lives of 14 and 2.5 h, respectively, were determined for the a-D-glucuronidases of C. stercorarium and T. saccharolyticurn. This description of a-D=glucuronidase activity in thermophilic anaerobic bacteria extends our knowledge of these enzymes, previously purified and characterized only in fungi.

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Section: Determination Of A-d-glucuronidase Activitymentioning

confidence: 99%

“…Reducing sugars were determined with the 3,5-dinitrosalicylic acid reagent (Wood & Bhat, 1988). Uronic acids were detected with the colorimetric reagent 3,s-dimethylphenol as described by Scott (1979). SDS-PAGE was performed in 10 % (w/v) polyacrylamide slab gels in the presence of 0.1 % SDS according to Laemmli (1970).…”

Section: Analytical Met Hodsmentioning

confidence: 99%

-D-Glucuronidases from the xylanolytic thermophiles Clostridium stercorarium and Thermoanaerobacterium saccharolyticum

Bronnenmeier

Meissner²,

Stöcker³

et al. 1995

Microbiology

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“…S/G ratio was evaluated according to Lin and Dence (1992) . Total uronic acids and acetyl groups were evaluated according to Scott (1979) and Solar et al (1987) , respectively.…”

Section: Methodsmentioning

confidence: 99%

Thermomechanical pulping of novel Brazilian Eucalyptus hybrids

Muguet¹,

Ruuttunen²,

Jääskeläinen³

et al. 2013

Holzforschung

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Abstract:The importance of Eucalyptus wood as a sustainable resource is well established. Mechanical pulp production is an energy-intensive process, and methods for decreasing energy demand are needed. In the present article, the structure of Eucalyptus hybrids and the produced pulps from the hybrids were assessed in terms of energy consumption, technical properties, and fiber morphology. The defibration and fiber development were investigated by traditional thermomechanical pulping in laboratory scale. There was no clear difference in the extent of defibration and fibrillation among the hybrids, with the exception of one hybrid (U2xGL1). Guaiacyl lignin contents of more than 7.2 % had a minor influence on energy consumption, and this finding is different from the results of previous studies on chemimechanical pulping processes. However, a low amount of guaiacyl lignin (6.1 % based on wood) decreased the energy demand. This assessment is of major importance concerning the energysaving defibration during thermomechanical pulping production.

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“…The cell wall polysaccharides in each fraction were analyzed colorimetrically for polyuronides using the 3,5-dimethylphenol method [12] and for total polysaccharides using the phenol-sulfuric acid method. α-D-Galacturonic acid and D-(+)-glucose were used as the standards for the polyuronide assay and the total polysaccharide assay, respectively.…”

Section: Determination Of Cell Wall Componentsmentioning

confidence: 99%