Colorimetric Determination of Sulfide in Microsamples

Sugahara, Shogo; Suzuki, Mai; Kamiya, Hiroshi; Yamamuro, Masumi; Semura, Hitoshi; Senga, Yukiko; Egawa, Michiko; Seike, Yasushi

doi:10.2116/analsci.32.1129

Cited by 28 publications

(16 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the promiscuous chemical properties make it difficult for accurate and reliable measurement of hydrogen sulfide in biological matrix15. Various methods have been established for endogenous hydrogen sulfide measurement, including using colorimetry16, gas chromatography17, electrodes selective for sulfide (ion-selective electrodes, ISEs)18, polarographic sensors19, fluorescent probes20, and high performance liquid chromatography (HPLC coupled with ultraviolet, fluorescence or electrochemical detection)2122. However, these methods have obvious limitation, such as complex preparation processes, low specificity and sensitivity, and time-consuming procedures15.…”

mentioning

confidence: 99%

New method for quantification of gasotransmitter hydrogen sulfide in biological matrices by LC-MS/MS

Tan

Jin

Sun

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Hydrogen sulfide exists widely in mammalian tissues and plays a vital role in physiological and pathophysiological processes. However, striking differences with orders of magnitude were observed for the detected hydrogen sulfide concentrations in biological matrices among different measurements in literature, which lead to the uncertainty for examination the biological relevance of hydrogen sulfide. Here, we developed and validated a liquid chromatography- mass spectrometry (LC-MS/MS) method for the determination of hydrogen sulfide in various biological matrices by determination of a derivative of hydrogen sulfide and monobromobimane named sulfide dibimane (SDB). 36S-labeled SDB was synthesized and validated for using as an internal standard. This method has been successfully used to measure hydrogen sulfide levels in a broad range of biological matrices, such as blood, plasma, tissues, cells, and enzymes, across different species. Moreover, a novel mode that hydrogen sulfide could loosely and non-covalently bind to human serum protein (HSA) and hemoglobin (HB) was revealed by using the developed method.

show abstract

mentioning

confidence: 99%

New method for quantification of gasotransmitter hydrogen sulfide in biological matrices by LC-MS/MS

Tan

Jin

Sun

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Stomatal movement is an important process in plants and influenced by external factors and H 2 S signaling has a key target in connection with guard cells (Jin and Pei ). Stomatal movement was also reported to ameliorate the effects of cadmium, aluminum, boron and chromium toxicity, temperature, water stress, osmotic stress, hypoxia and other stresses (Qian et al , Sugahara et al , Tan et al ). In response to environmental stresses, H 2 S plays an essential role as it was also found to act as important cellular signal and has a protective effect against heavy metal stress (Faro et al ).…”

Section: Contribution Of H2s and No In The Regulation Of Plant Develomentioning

confidence: 99%

Revealing on hydrogen sulfide and nitric oxide signals co‐ordination for plant growth under stress conditions

Singh

Kumar

Kapoor

et al. 2019

Physiologia Plantarum

View full text Add to dashboard Cite

In the recent times, plants are facing certain types of environmental stresses, which give rise to formation of reactive oxygen species (ROS) such as hydroxyl radicals, hydrogen peroxides, superoxide anions and so on. These are required by the plants at low concentrations for signal transduction and at high concentrations, they repress plant root growth. Apart from the ROS activities, hydrogen sulfide (H2S) and nitric oxide (NO) have major contributions in regulating growth and developmental processes in plants, as they also play key roles as signaling molecules and act as chief plant immune defense mechanisms against various biotic as well as abiotic stresses. H2S and NO are the two pivotal gaseous messengers involved in growth, germination and improved tolerance in plants under stressed and non‐stress conditions. H2S and NO mediate cell signaling in plants as a response to several abiotic stresses like temperature, heavy metal exposure, water and salinity. They alter gene expression levels to induce the synthesis of antioxidant enzymes, osmolytes and also trigger their interactions with each other. However, research has been limited to only cross adaptations and signal transductions. Understanding the change and mechanism of H2S and NO mediated cell signaling will broaden our knowledge on the various biochemical changes that occur in plant cells related to different stresses. A clear understanding of these molecules in various environmental stresses would help to confer biotechnological applications to protect plants against abiotic stresses and to improve crop productivity.

show abstract

“…It is important to investigate the levels of H 2 S in blood and tissue for its physiological functions. There are several analytical methods to detect H 2 S concentration, such as colorimetry [ 10 ], fluorescent probes [ 11 ], liquid chromatography-mass spectrometry [ 12 ], spectrophotometric analysis [ 13 ], silver sulfide or polarographic sensor [ 14 , 15 ], and headspace gas determination [ 16 ]. Different analysis technologies got the different H 2 S concentrations.…”

Section: Physical Properties Biosynthesis and Reactivity Of H mentioning

confidence: 99%

An Update on Hydrogen Sulfide and Nitric Oxide Interactions in the Cardiovascular System

Zhu

2018

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Hydrogen sulfide (H2S) and nitric oxide (NO) are now recognized as important regulators in the cardiovascular system, although they were historically considered as toxic gases. As gaseous transmitters, H2S and NO share a wide range of physical properties and physiological functions: they penetrate into the membrane freely; they are endogenously produced by special enzymes, they stimulate endothelial cell angiogenesis, they regulate vascular tone, they protect against heart injury, and they regulate target protein activity via posttranslational modification. Growing evidence has determined that these two gases are not independent regulators but have substantial overlapping pathophysiological functions and signaling transduction pathways. H2S and NO not only affect each other's biosynthesis but also produce novel species through chemical interaction. They play a regulatory role in the cardiovascular system involving similar signaling mechanisms or molecular targets. However, the natural precise mechanism of the interactions between H2S and NO remains unclear. In this review, we discuss the current understanding of individual and interactive regulatory functions of H2S and NO in biosynthesis, angiogenesis, vascular one, cardioprotection, and posttranslational modification, indicating the importance of their cross-talk in the cardiovascular system.

show abstract

Colorimetric Determination of Sulfide in Microsamples

Cited by 28 publications

References 10 publications

New method for quantification of gasotransmitter hydrogen sulfide in biological matrices by LC-MS/MS

New method for quantification of gasotransmitter hydrogen sulfide in biological matrices by LC-MS/MS

Revealing on hydrogen sulfide and nitric oxide signals co‐ordination for plant growth under stress conditions

An Update on Hydrogen Sulfide and Nitric Oxide Interactions in the Cardiovascular System

Contact Info

Product

Resources

About