Colorimetric determination of urinary oxalate recovered as calcium oxalate

Baadenhuijsen, H.; Jansen, A.P.

doi:10.1016/0009-8981(75)90243-0

Cited by 28 publications

(2 citation statements)

References 14 publications

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“…The determination of oxalates in biological materials is usually performed by indirect colorimetry and by precipitation of calcium oxalate followed by weighing or titration. A huge number of articles related to the determination of calcium oxalate are freely available, for example, its determination in urine [7] and blood plasma [8]. In addition, oxalic acid was quantified by capillary gas chromatography in plant tissues.…”

Section: Introductionmentioning

confidence: 99%

Kinetic Photometric Determination of Oxalate by Its Activation Effect on Catalytic Oxidation of Iodide and Formation of Iodine-Starch Complex

Danilina,

Khaybullina

2021

Chem

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A method for the kinetic photometric determination of oxalate ion in aqueous solutions, in-cluding extracts from foodstuffs, has been suggested. The initial rate method has been used: the straight parts of kinetic curves (approximately 5 min) are processed by the least square method to get the slope coefficients used as analytical signals. The procedure is based upon the oxalate ion action as an activator in the iron(II)-catalyzed reaction of iodide oxidation by potassium bromate. Unlike the previously used light absorbance in the UV region, the suggested procedure controls the rate of the iodine-starch complex formation at 590 nm. The greatest initial rate for the reaction is at pH 5.05 of the acetate buffer solution and the optimal concentration of soluble starch 0.30 mg/mL. The calibration graph is linear in the oxalate concentration range (0.1–10) mg/mL. In this interval precision error varies between 0.67 % and 4.71 %, accuracy error is within (0.22–9.30) %. The procedure has been applied to food analysis by the standard addition method. Aqueous extract has been obtained from 2.5 g of finely chopped sample of raw beet, stirred with 100 mL of water for 1 h, centrifuged, filtered, and quantitatively transferred to 250-mL volumetric flask. Determination of oxalate in 1-mL aliquot has required the same volume of colored beet extract for a reference solution, but otherwise the procedure stands valid. The oxalate content has been found to equal (379 ± 2) mg/g of the raw beet (P = 0,95; n = 4), with 0.6 % precision error and 3.0 % accuracy error.

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Section: Introductionmentioning

confidence: 99%

Kinetic Photometric Determination of Oxalate by Its Activation Effect on Catalytic Oxidation of Iodide and Formation of Iodine-Starch Complex

Danilina,

Khaybullina

2021

Chem

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“…Hence gas chromatographic [8][9][10], high performance liquid chromatographic [11][12][13], ion pair chromatographic [14][15] methods have been developed for analysis of oxalate from biological fluids. The various other enzymatic [16][17][18][19][20][21], colorimetric [22,23] and Isotachophoresis [24,25] methods have been developed for analysis of oxalate from biological fluids and in the presence of oxalate concentration altering bio-molecules like ascorbic acid. Among all these methods, oxalate oxidase (OO) method was most widely used as it was developed in the form of a colorimetric kit which will give accurate, robust and uniform result when you analyze the same at any centre over the entire globe.…”

Section: Introductionmentioning

confidence: 99%

HPLC Analysis of Human Urine for Oxalate Content

Suryavanshi

Jadhav

Gune

et al. 2016

Int J Pharm Pharm Sci

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Objective: In the present communication, development and validation of reverse phase-high performance liquid chromatography method have been carried out for estimation of oxalate content in the urine of human volunteers with recurrent kidney stone disease and healthy status. Methods:The analysis of oxalic acid has been carried out on KYA TECH HiQ Sil C18HS column using a mobile phase of methanol: 0.001 N acetic acid in water (50:50, v/v) with a flow rate of 1 ml/min and detection wavelength, 237 nm.Results: Analysis of oxalate content was carried out using single point calibration method with retention at 2.705 min with good resolution parameters. Urine sample collected from kidney stone patients and healthy volunteers over the period of 24 h were analyzed and it has been found that concentration of oxalate in healthy volunteers is less than 12 µg/ml whereas that in case of kidney stone patients is in the range of 39-151 µg/ml and this data can be utilized for further interpretations about oxalate content in healthy and kidney stone diseased volunteers. This method was validated as per united states food and drug administration (USFDA) guidelines by the study of accuracy, precision, linearity, range, selectivity, the lower limit of quantitation, extraction recovery studies and stability studies for determining oxalate content in the urine of human volunteers. As relative standard deviations of oxalate content estimated are less than 5 percent, the method can be claimed accurate, precise, sensitive and selective for determining oxalate content in the urine of human volunteers. Conclusion:The results are satisfactory, proving the effectiveness of the method for analysis of oxalate content from other biological fluids with few optimizations.

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Assay of Oxalate and Glycollate in Urine

Kasidas¹

1988

Oxalate Metabolism in Relation to Urinary Stone

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Colorimetric determination of urinary oxalate recovered as calcium oxalate

Cited by 28 publications

References 14 publications

Kinetic Photometric Determination of Oxalate by Its Activation Effect on Catalytic Oxidation of Iodide and Formation of Iodine-Starch Complex

Kinetic Photometric Determination of Oxalate by Its Activation Effect on Catalytic Oxidation of Iodide and Formation of Iodine-Starch Complex

HPLC Analysis of Human Urine for Oxalate Content

Assay of Oxalate and Glycollate in Urine

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