“…Hydrogen peroxide (H 2 O 2 ), as one of the reactive oxygen species, is generated from oxygen metabolism in cells and closely associated with signal transduction and cell growth. , Mounting evidence indicates that H 2 O 2 can be recognized as one of the biomolecule biomarkers in live cells for many diseases, as overproduction of H 2 O 2 could cause damage to nucleic acids, proteins, brain, and tissues, and then lead to a series of diseases, such as Parkinson’s disease and Alzheimer disease. − Therefore, it is essential to detect H 2 O 2 quantitatively in cells for full understanding of its biological effects in cellular physiology. Currently, numerous sensors based on different analytical techniques, such as colorimetry, , electrochemistry, , fluorescence, , and chemiluminescence, have been developed to detect H 2 O 2 in the cellular environment. However, cells were usually in solution due to the weak adhesion and poor biocompatibility of support materials for cells’ immobilization in most of these studies, resulting in a long diffusion distance to reaction sites for the biomolecule, which could further affect the detection sensitivity.…”