Column-chromatographic extraction and separation of polyphenols, caffeine and theanine from green tea

Wang, Li; LiHong, Gong; Chen, Chang-Jian; Hanbing, Han; Li, Hai-Hang

doi:10.1016/j.foodchem.2011.09.129

Cited by 76 publications

(46 citation statements)

References 10 publications

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“…The associated results are reported in Figure 2B (detailed at Table S5 in Supporting Information), using K 3 PO 4 at 20.0 wt% (the best condition optimised above). The increase in the 2-propanol concentration is responsible for the increase of In order to conclude about the performance of our partition systems, the values of R T achieved in this work needs to be analysed and compared with those found in literature [3,5,7]. Despite the fact that, the values of R T increase due to the increase of the volume of the bottom phase, it is possible to compare our results with some of the recovery results found in literature.…”

Section: Caffeine Partition Into the Alcohol-rich Phasementioning

confidence: 69%

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Selective partition of caffeine from coffee bean and guaraná seed extracts using alcohol–salt aqueous two-phase systems

Santos

Reis

Silva

et al. 2016

Separation Science and Technology

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This work proposes the application of flexible alcoholic aqueous two-phase systems (ATPS) to manipulate the partition of caffeine. An optimization study was performed the caffeine partitioning towards top phase (60.0 wt% 2-propanol + 20.0 wt% K 3 PO 4 at 303 K -K caf = 3.4 and R T =89.1%) and bottom phase (60.0 wt% methanol + 17.5 wt% K 2 HPO 4 /KH 2 PO 4 at 278 K -K caf = 0.06 and R B = 81.1%). These processes were evaluated by applying them to real biomass as guaraná seeds (purification factor -FP = 6.59 fold) and coffee beans (PF = 3.24 fold) for the 2-propanol and K 2 HPO 4 /KH 2 PO 4 system.

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Section: Caffeine Partition Into the Alcohol-rich Phasementioning

confidence: 69%

“…Other unconventional approaches propose the use of high temperature and ultrasounds [6], column chromatography [7], microwave-enhanced vacuum ice water extraction [8], and aqueous solution of ionic liquids [9]. However, to enhance the purity level of caffeine, a purification step must be applied in most cases to the crude extract.…”

Section: Introductionmentioning

confidence: 99%

Selective partition of caffeine from coffee bean and guaraná seed extracts using alcohol–salt aqueous two-phase systems

Santos

Reis

Silva

et al. 2016

Separation Science and Technology

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“…To reduce the production cost on the basis of the separation effect, some key parameters were optimized in previous work. For instance, two height‐to‐diameter (H/D) ratios (5:1 and 10:1) of the column were tested for the extraction of substances, and a similar optimization experiment has been reported . Therefore, three H/D ratios of the column (4:1, 5.5:1 and 7:1) were employed in this experiment.…”

Section: Resultsmentioning

confidence: 99%

Separation of α‐ketoglutaric acid and pyruvic acid from the culture broth of Yarrowia lipolytica WSH‐Z06 by chromatographic methods

Peng

Zeng

et al. 2018

Biotechnology Progress

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Both α‐ketoglutaric acid (KGA) and pyruvic acid (PYR) are important keto acids. Efficient co‐production of KGA and PYR has been achieved in our previous work, and could significantly decrease the cost of fermentation production. KGA and PYR have similar physical and chemical properties. Hence, finding a way to separate the two keto acids efficiently has become a key challenge. In this study, different chromatographic methods have been investigated, including ion‐exchange chromatography, aluminum oxide chromatography and silica gel chromatography. The results show that the two keto acids can be well separated with silica gel chromatography, whereas ion‐exchange chromatography and aluminum oxide chromatography could not separate them. Using the pretreated fermentation broth of Yarrowia lipolytica WSH‐Z06, the purity and yield of KGA/PYR reached 98.7%/99.1% and 86.7%/70.9%, respectively, with an optimized silica gel chromatography‐based procedure. This study provides an efficient method for separating KGA and PYR from fermentation broth, which might be applied on an industrial scale and significantly decrease the cost of biotechnological production of keto acids. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:1370–1379, 2018

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“…The CCE was performed in glass chromatographic columns as described previously (Wang et al ., ). For non‐cyclic CCE, plant material (3 g unless for scaled‐up experiments) was loaded into a column with a minimum volume (MV) of solvent (60% or 70% ethanol) through the wet column preparation method in column chromatography.…”

Section: Methodsmentioning

confidence: 97%

New and Highly Efficient Column Chromatographic Extraction and Simple Purification of Camptothecin from Camptotheca acuminata and Nothapodytes pittosporoides

Xuehua

et al. 2013

Phytochemical Analysis

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Column-chromatographic extraction and separation of polyphenols, caffeine and theanine from green tea

Cited by 76 publications

References 10 publications

Selective partition of caffeine from coffee bean and guaraná seed extracts using alcohol–salt aqueous two-phase systems

Selective partition of caffeine from coffee bean and guaraná seed extracts using alcohol–salt aqueous two-phase systems

Separation of α‐ketoglutaric acid and pyruvic acid from the culture broth of Yarrowia lipolytica WSH‐Z06 by chromatographic methods

New and Highly Efficient Column Chromatographic Extraction and Simple Purification of Camptothecin from Camptotheca acuminata and Nothapodytes pittosporoides

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