New and Highly Efficient Column Chromatographic Extraction and Simple Purification of Camptothecin from Camptotheca acuminata and Nothapodytes pittosporoides

Abstract

The new procedures are simple and highly efficient, and have multiple advantages for quantitative analysis and large production of camptothecin from plants.

Chemical constituents of Nothapodytes pittosporoides (Icacinaceae)

“…CIDs were also reported from N. foetida (Govindachari and Viswanathan, 1972) and N. pittosporoides (Lv et al, 2010;Zeng et al, 2013;Bai and Song, 2014). Interestingly, CIDs have never been reported from any other species of Nothapodytes.…”

Alkaloid purification using rosin‐based polymer‐bonded silica stationary phase in HPLC

“…A total of 50 g dried whole camptotheca fruit and evodiae were powered and extracted using 300 mL of methanol three times in Soxhlet apparatus. Green tea was added to 300 mL of water and the resulting solution was boiled for 10 min .…”

Simultaneous extraction of nicotine and solanesol from waste tobacco materials by the column chromatographic extraction method and their separation and purification

“…The CCE method includes two steps, dissolving and eluting the target compounds from the plant material in chromatographic columns with minimum volume solvent at room temperature [21][22][23]. As shown in Fig.…”

“…CCE was performed in glass chromatographic columns [21][22][23]. For non-cyclic CCE, 8 g tobacco material was loaded into a column with one MV solvent at a bed height to diameter ratio of 10:1 or 5:1, through the wet column preparation method.…”

“…We have reported a highly efficient column-chromatographic extraction (CCE) method for the extraction of natural compounds from biological materials [21][22][23]. In this work, we report a unique procedure for simultaneously extracting nicotine and solanesol and hydrolyzing bound forms or ester forms of nicotine and solanesol using a minimum volume of the optimal solvent, followed by automatic separation and simple purification to economically produce highly-purified nicotine and solanesol using the waste tobacco materials.…”