Column chromatography and HPLC analysis of phenolic compounds in the fractions of Salvinia molesta mitchell

Abstract

Salvinia molesta, commonly known as giant Salvinia, is a floating fern belonging to the family of Salviniaceae. In this study the active fractions of the fern extract were separated using column chromatography and phenolic compounds present in the active fractions were determined by RP-HPLC. Ethyl acetate extract was found to possess significant pharmacological activity when compared to other extracts under study and therefore an attempt was made to fractionate ethyl acetate extract. The analysis was performed through two different mobile phases involving solvent A (acetonitrile) and solvent B (0.1% phosphoric acid in water) and solvent A (methanol) and Solvent B (4% acetic acid). HPLC analysis indicated the presence of phenolic compounds namely ascorbic acid, quercetin, gallic acid, resorcinol, catechol, vanillin and benzoic acid with specific retention times. The detected compounds possess antioxidant and antitumour activities. The results of the present study suggests the possibility to use S. molesta as a source for a plausible antioxidant agent which could be isolated and used as a lead candidate for the development of antioxidant drugs that help stop or limit damage caused by free radicals and to counteract oxidative stress leading to the prevention of a variety of chronic and degenerative diseases.

Economical Performances and Cholesterol Profiles due to the Incorporation of Salvinia molesta in the Diets of Kampong Chicken

“…The utilization of S. molesta in the ration of Kampong chicken increased body weight and FCR. These results are supported by the findings of Dwiloka et al (2015), Moozhiyil & Pallauf (1986), Gini & Jothi (2018), and Brouwer (2019) that argue that SML as an aquatic plant contains high fiber and tannin which will increase nutrient utilization and ultimately lead to a poor FCR, but with a better weight in duck performance. Even though the FCR is increased by the use of S. molesta, breast muscle and abdominal fat are also getting higher.…”

Antifungal efficacy of Thevetia peruviana leaf extract against Alternaria solani and characterization of novel inhibitory compounds by Gas Chromatography-Mass Spectrometry analysis

“…Antimicrobial activity of plants extract may be attributed instead of existence of active ingredients in prepared infusions. Extraction processes usually used to extract active ingredients are crude extraction and partially purified extraction process [ 17 , 39 , 40 ]. The principle of series of extraction process is to isolate the soluble plant metabolites, and left behind the insoluble cellular mass (residue).…”

Allergenic components, biocides, and analysis techniques of some essential oils used in food products

“…The study of the first column chromatography done was about allergenic constituents by Grob et al (Grob et al, 1981). There are some studies in the literature related to phenolic compound analysis in HPLC (Gini & Jeya Jothi, 2018;Lazarova et al, 2014;Skendi et al, 2017). Some of the alcohol derivatives' concentrations such as benzyl alcohol, geraniol, and cinnamyl alcohol were analyzed by HPLC in former studies and their concentrations were determined.…”