Column-switching technique for the sensitive determination of ertapenem in human cerebrospinal fluid using liquid chromatography and ultraviolet absorbance detection

Assay of ertapenem in human serum by high-performance liquid chromatography

“…Chromatographic methods have been described for the assay of ertapenem and, although these appear to be slightly more sensitive [10] than the method described here, they are more complex. The previously described methods use column switching that requires an advanced system of two pumps and different mobile phases [9,10], which may not be available in many laboratories. In this study, we have been able to develop a simple, straightforward method to assay ertapenem in human serum that is able to detect ertapenem at clinically relevant concentrations.…”

“…Although high-performance liquid chromatography (HPLC) methods have been described for ertapenem, these are complex and involve column switching and thus this type of assay may not suitable for use in routine clinical microbiology laboratories [9,10]. In this study we report a rapid, straightforward HPLC method for assay of ertapenem in human serum.…”

A new simple HPLC assay for the quantification of ertapenem in human plasma, lung tissue, and broncho-alveolar lavage fluid

“…In a general modification of the methods described before [3][4][5][6][7][8] an HPLC column of 3.0 mm I.D. with an appropriate lining (0.13 mm I.D.)…”

“…The capabilities of ertapenem regarding the penetration into tissues or lumina, and the permeation of cell membranes into the inner compartment are expected to differ markedly from imipenem or meropenem and need to be addressed in separate studies which require a broad array of analytical methods. However, only few HPLC/UV methods for the determination of ertapenem in biological matrices are published [3][4][5][6][7][8]. All are based on standard 4.6 mm I.D.…”

Sensitive column-switching high-performance liquid chromatography method for determination of propiverine in human plasma

“…More sensitive and specific methods are therefore required for the analysis of PPV in plasma. Recently, a number of papers have been published on the subject of HPLC column-switching [9][10][11][12][13]. Some of these papers showed that HPLC column-switching method can increase the sensitivity and specificity [9,10].…”