Combination of 15 lipid metabolites and motilin to diagnose spleen-deficiency FD

Zhang, Jiaqi; Shi, Xiaoshuang; Xie, Jingyi; Zhang, Min; Ma, Jinxin; Wang, Fengyun; Tang, Xudong

doi:10.1186/s13020-019-0238-9

Cited by 11 publications

(7 citation statements)

References 34 publications

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“…The symptom of abdominal distention suggests that the Qi of the spleen and stomach are stagnant and fail to transport food, consequently manifesting a feeling of fullness or distention in the abdomen. Currently, the widely used assays for the spleen and stomach Qi stagnation involve gastric emptying and small bowel transit (J. Q. Zhang et al, ). Notably, in this study, 6‐week stressed rats showed an evident symptom of abdominal distention with significant inhibition of gastric emptying and small bowel transit.…”

Section: Discussionmentioning

confidence: 99%

A combination of depression and liver Qi stagnation and spleen deficiency syndrome using a rat model

Qiu

et al. 2020

The Anatomical Record

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A syndrome (Zheng in Chinese) plays a critical role in disease identification, diagnosis, and treatment in traditional Chinese medicine (TCM). Clinically, the liver Qi stagnation and spleen deficiency syndrome (LQSSDS) is one of the most common syndrome patterns. Over the past few decades, several animal models have been developed to understand the potential mechanisms of LQSSDS, but until now, simulation of the syndrome is still unclear. Recently, several studies have confirmed that an animal model combining a disease and a syndrome is appropriate for simulating TCM syndromes. Overlapping previous studies have reported that depression is highly associated with LQSSDS; hence, we attempted to develop a rat model combining depression and LQSSDS. We exposed the rats to different durations of chronic unpredictable mild stress (CUMS). Subsequently, the evaluation indicators at macrolevel consisted of behavioral tests including open field test, sucrose preference test, and forced swim test, food intake, body weight, white adipose tissue, fecal water content, visceral hypersensitivity, and small bowel transit, and the evaluation indicators at microlevel included changes of hypothalamic–pituitary–adrenal axis. Serum D‐xylose absorption was used to comprehensively confirm and assess whether the model was successful during the CUMS‐induced process. The results showed that rats exposed to 6‐week CUMS procedure exhibited significantly similar traits to the phenotypes of LQSSDS and depression. This study provided a new rat model for the LQSSDS and could potentially lead to a better understanding of the pathophysiology of LQSSDS and the development of new drugs for this syndrome.

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Section: Discussionmentioning

confidence: 99%

A combination of depression and liver Qi stagnation and spleen deficiency syndrome using a rat model

Qiu

et al. 2020

The Anatomical Record

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“…With the development of modern research in TCM, metabolomics is increasingly being applied to the study of syndrome differentiation in TCM, such as spleen-deficiency and qi-stagnation. 10 , 18 However, there have been no reports on the mechanism research or related metabolomics studies regarding the pathogenesis of gallbladder heat and spleen cold syndrome, which is one of the most common clinical syndromes. Furthermore, Chaihu Guizhi Ganjiang Decoction, as a classic formula for treating digestive system disorders, also lacks any relevant metabolomics reports regarding its therapeutic effects on gastric diseases.…”

Section: Discussionmentioning

confidence: 99%

The Efficacy of Chaihu-Guizhi-Ganjiang Decoction on Chronic Non-Atrophic Gastritis with Gallbladder Heat and Spleen Cold Syndrome and Its Metabolomic Analysis: An Observational Controlled Before-After Clinical Trial

Wen,

Liu,

Pang

et al. 2024

DDDT

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Purpose The aim of this study was to verify the effectiveness and explore the mechanism of Chaihu-Guizhi-Ganjiang decoction (CGGD) in the treatment of chronic non-atrophic gastritis (CNAG) with gallbladder heat and spleen cold syndrome (GHSC) by metabolomics based on UHPLC-Q-TOF/MS. Patients and Methods An observational controlled before-after study was conducted to verify the effectiveness of CGGD in the treatment of CNAG with GHSC from January to June 2023, enrolling 27 patients, who took CGGD for 28 days. 30 healthy volunteers were enrolled as the controls. The efficacy was evaluated by comparing the traditional Chinese medicine (TCM) syndrome and CNAG scores, and clinical parameters before and after treatment. The plasma levels of hormones related to gastrointestinal function were collected by ELISA. The mechanisms of CGGD in the treatment of CNAG with GHSC were explored using a metabolomic approach based on UHPLC-Q-TOF/MS. Results Patients treated with CGGD experienced a statistically significant improvement in TCM syndrome and CNAG scores (p < 0.01). CGGD treatment evoked the concentration alteration of 15 biomarkers, which were enriched in the glycerophospholipid metabolism, and branched-chain amino acids biosynthesis pathways. Moreover, CGGD treatment attenuated the abnormalities of the gastrointestinal hormone levels and significantly increased the pepsinogen level. Conclusion It was the first time that this clinical trial presented detailed data on the clinical parameters that demonstrated the effectiveness of CGGD in the treatment of CNAG with GHSC patients. This study also provided supportive evidence that CNAG with GHSC patients were associated with disturbed branched-chain amino acid metabolism and glycerophospholipid levels, suggesting that CNAG treatment based on TCM syndrome scores was reasonable and also provided a potential pharmacological mechanism of action of CGGD.

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“…A comparison of metabolic pathways showed that ginseng could mainly correct three metabolic pathways, such as glyceride metabolism, taurine and hypotaurine metabolism, and beta‐alanine metabolism in rats with spleen‐qi deficiency. Glyceride metabolism is a common metabolic pathway which is related to the synthesis of glycerides and energy metabolism [27]. The contents of glycerol and dihydroxyacetone in the urine of spleen‐qi‐deficient rats were lower than that of normal rats.…”

Section: Discussionmentioning

confidence: 99%

Metabolomics and network pharmacological analysis to explore the mechanism of ginseng treatment for spleen‐qi deficiency

Liu

Cai

et al. 2022

J of Separation Science

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Ginseng is the main Chinese herbal medicine for tonifying Qi and invigorating the spleen. It has been used to treat spleen‐qi deficiency with good protective effects for thousands of years, however, its biological mechanism has not been fully elucidated. This study aims to explore the mechanism of ginseng in the treatment of spleen‐qi deficiency by using a comprehensive method combining metabolomics and network pharmacological analysis. Gas chromatography‐mass spectroscopy was applied for investigating the changes in urine metabolites in spleen‐qi deficiency rats and after treatment with ginseng. Metabolomics and network pharmacology analysis were applied to screen potential biomarkers and therapeutic targets of ginseng in the treatment of spleen‐qi deficiency, respectively. Molecular docking was employed to further evaluate the docking mode of potential biomarkers and therapeutic target proteins. The results of metabolomics showed that the therapeutic effects of ginseng are mainly related to its regulation of three metabolic pathways. The molecular structure of potential biomarkers and common proteins was further analyzed by molecular docking to verify its effectiveness. Ginseng has good pharmacological effects by controlling key targets of related metabolic pathways, signal pathways, and potential biomarkers.

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Combination of 15 lipid metabolites and motilin to diagnose spleen-deficiency FD

Cited by 11 publications

References 34 publications

A combination of depression and liver Qi stagnation and spleen deficiency syndrome using a rat model

A combination of depression and liver Qi stagnation and spleen deficiency syndrome using a rat model

The Efficacy of Chaihu-Guizhi-Ganjiang Decoction on Chronic Non-Atrophic Gastritis with Gallbladder Heat and Spleen Cold Syndrome and Its Metabolomic Analysis: An Observational Controlled Before-After Clinical Trial

Metabolomics and network pharmacological analysis to explore the mechanism of ginseng treatment for spleen‐qi deficiency

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