Metabolomics and network pharmacological analysis to explore the mechanism of ginseng treatment for spleen‐qi deficiency

Liu, Ming; Cai, Yongyu; Li, Fangtong; Wu, Yongxi; Zhang, Meiyu; Wang, Yizhu; Fan, Meiling; Li, Bo; Huang, Xin; Zheng, Fei; Yue, Hao

doi:10.1002/jssc.202200457

Cited by 7 publications

(6 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IL2 is necessary for T cell proliferation and other immune regulation [25]. EGFR and PTGS2 regulate multiple anti-inflammatory and immunomodulatory signaling pathways [45]. GO and KEGG enrichment analysis showed that the signaling pathways were mainly enriched in viral infection, bacterial infection, inflammatory pathway, immune pathway, and cancer, which may be related to the anti-inflammatory pathway of immune cells where TNF is located.…”

Section: Discussionmentioning

confidence: 99%

An integrated approach of high‐performance liquid chromatography–mass spectrometry‐based chemical profiling, network pharmacology, and molecular docking to reveal the potential mechanisms of Qishen Gubiao granules for treating coronavirus disease 2019

Yang

Zhang

et al. 2023

J of Separation Science

View full text Add to dashboard Cite

a traditional Chinese medicine preparation composed of nine herbs, have been widely used to prevent and treat coronavirus disease 2019 with good clinical efficacy. In the present study, an integrated strategy based on chemical profiling followed by network pharmacology and molecular docking was employed, to explore the active components and potential molecular mechanisms of Qishen Gubiao granules in the therapy of coronavirus disease 2019. Using the ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry technique, a total of 186 ingredients corresponding to eight structure types in Qishen Gubiao preparation were identified or structurally annotated with the elucidation of the fragmentation pathways in the typical compounds. The network pharmacology analysis screened 28 key compounds including quercetin, apigenin, scutellarein, luteolin and naringenin acting on 31 key targets, which possibly modulated signal pathways associated with immune and inflammatory responses in the treatment of coronavirus disease 2019. The molecular docking results observed that the top 5 core compounds

show abstract

Section: Discussionmentioning

confidence: 99%

An integrated approach of high‐performance liquid chromatography–mass spectrometry‐based chemical profiling, network pharmacology, and molecular docking to reveal the potential mechanisms of Qishen Gubiao granules for treating coronavirus disease 2019

Yang

Zhang

et al. 2023

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Liu et al. [8, 9] used GC‐MS to study the blood and urine of SQD rats and confirmed that the therapeutic effects of ginseng could be achieved by modulating multiple metabolic pathways. Therefore, ginseng and its active ingredients can improve the intestinal environment, regulate the structure of the flora, and improve metabolic diseases.…”

Section: Introductionmentioning

confidence: 99%

Urine and serum metabolomics study of wild ginseng in rats with spleen‐qi deficiency using rapid resolution liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

Zhang

et al. 2023

J of Separation Science

Self Cite

View full text Add to dashboard Cite

Patients with a spleen-qi deficiency often exhibit dysfunction in the metabolic system. Metabolites are considered the most direct reflection of individual physiological and pathological conditions and represent attractive candidates to provide deep insights into disease phenotypes. This study examines the potential therapeutic mechanism of wild ginseng on spleen-qi deficiency through the analysis of serum and urine metabolomics using rapid-resolution liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry. The reasons for the superiority of wild ginseng treatment over cultivated ginseng were also analyzed in depth. After wild ginseng intervention, anandamide, urobilinogen, aldosterone, and testosterone glucuronide were significantly reduced in serum. Meanwhile, argininosuccinic acid, L-cysteine, and seven other metabolites were significantly elevated in serum. Nine metabolites, including Lacetylcarnitine, and citrulline were elevated in the urine, and trimethylamine N-oxide, adrenic acid, and 10 other metabolites were reduced. Arginine biosynthesis, pantothenate and CoA biosynthesis, thiamin metabolism, taurine, and tryptophan metabolism pathways were mainly improved. Further analysis was conducted on the relationship between Lactobacillus and Akkermansia bacteria with metabolites, and it was found that they are mainly related to amino acid metabolites. This study provides strong theoretical support and direction for further explanation of the immune mechanism of wild ginseng and lays the foundation for future studies.

show abstract

“…Gas chromatography–mass spectrometry (GC/MS) metabolomics was adopted to study the metabolic profile changes in the urine of rats with spleen‐qi deficiency after ginseng treatment. Potential biomarkers were found to have participated in glycerolipid, taurine and hypotaurine, and β‐alanine metabolic pathways 20 …”

Section: Introductionmentioning

confidence: 99%

“…Potential biomarkers were found to have participated in glycerolipid, taurine and hypotaurine, and β-alanine metabolic pathways. 20 In the study reported here, we sequenced the microbial DNA extracted from the feces of experimental animals by 16s rRNA highthroughput sequencing technology (Illumina platform). The changes of SCFAs in intestinal contents were detected by GC/MS techniques.…”

mentioning

confidence: 99%

Effects of ginseng on short‐chain fatty acids and intestinal microbiota in rats with spleen‐qi deficiency based on gas chromatography–mass spectrometry and 16s rRNA technology

Zhang,

Pi,

et al. 2023

Rapid Comm Mass Spectrometry

Self Cite

View full text Add to dashboard Cite

RationaleSpleen‐qi deficiency syndrome, a common weakness syndrome in traditional Chinese medicine, results from insufficient spleen‐qi levels. For centuries, ginseng has been relied upon as a traditional Chinese medicine to treat spleen‐qi deficiency syndrome. Until now, the mechanism feature of ginseng in treating temper deficiency through intestinal bacteria and short‐chain fatty acid (SCFA) metabolites has not been fully elucidated.MethodsThis study established a rat model of spleen‐qi deficiency via multi‐factor compound modeling that involved fatigue injury and a controlled diet. The content of SCFAs between different treatment groups was determined by gas chromatography–mass spectrometry. And the 16s rRNA sequencing technology was applied to reveal the effects of ginseng on the intestinal microecological environment of the rats.ResultsIt was found that the ginseng treatment group exhibited the most remarkable regulatory effect on propionic acid, surpassing all other administration groups. Ginseng increased the relative abundance of beneficial bacteria and decreased that of harmful bacteria at the genus level in rats with spleen‐qi deficiency syndrome. And propionic acid is significantly positively correlated with Lactobacillus level and significantly negatively correlated with uncultured_bacterium_f_Muribaculaceae (p < 0.05). n‐Butyric acid is negatively correlated with the Faecalibaculum level (p < 0.01). n‐Valeric acid is significantly negatively correlated with the Romboutsia level (p < 0.01).ConclusionThe mechanism of ginseng treatment for spleen‐qi deficiency is elucidated from the perspective of gut microbiota and its metabolite SCFAs. It provides a new way for further development and utilization of ginseng and a theoretical basis.

show abstract

Metabolomics and network pharmacological analysis to explore the mechanism of ginseng treatment for spleen‐qi deficiency

Cited by 7 publications

References 49 publications

An integrated approach of high‐performance liquid chromatography–mass spectrometry‐based chemical profiling, network pharmacology, and molecular docking to reveal the potential mechanisms of Qishen Gubiao granules for treating coronavirus disease 2019

An integrated approach of high‐performance liquid chromatography–mass spectrometry‐based chemical profiling, network pharmacology, and molecular docking to reveal the potential mechanisms of Qishen Gubiao granules for treating coronavirus disease 2019

Urine and serum metabolomics study of wild ginseng in rats with spleen‐qi deficiency using rapid resolution liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

Effects of ginseng on short‐chain fatty acids and intestinal microbiota in rats with spleen‐qi deficiency based on gas chromatography–mass spectrometry and 16s rRNA technology

Contact Info

Product

Resources

About