Combination of 2,4-D and stress improves indirect somatic embryogenesis in<i>Cattleya maxima</i>Lindl.

Cueva-Agila, Augusta; Guachizaca, I.; Cella, Rino

doi:10.1080/11263504.2013.797033

Cited by 13 publications

(5 citation statements)

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“…The maximum number of embryos was obtained on a medium containing (3.39 μM) 2,4-D and (6.80 μM) TDZ. However, in an earlier report of somatic embryogenesis in C. cristata and C. maxima [29], [35] a lesser number of embryoids were produced compared to our study. Thus, our protocol proved to be more effective for efficient embryogenesis in M. densiflora .…”

Section: Discussioncontrasting

confidence: 94%

“…Somatic embryos production followed by somatic development has been reported in several orchid plants using different explants, such as protocorms in Cymbidium [24], Phalaenopsis amabilis var. formosa [25], Rhynchostylis gigantea [26], Cymbidium bicolor [27], Phalaenopsis Richard Shaffer Santa Cruz [28], Cattleya maxima [29] and Phalaenopsis aphrodite [30].…”

Section: Discussionmentioning

confidence: 99%

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Direct somatic embryogenesis of Malaxis densiflora (A. Rich.) Kuntze

Mahendran

Bai

2016

Journal of Genetic Engineering and Biotechnology

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A protocol for induction of direct somatic embryogenesis and subsequent plant regeneration for the medicinally important and endangered plant of Malaxis densiflora has been developed for the first time. In the present study, in vitro seed derived protocorm explants were cultured on half strength Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), Picloram and Dicamba individually and in combination with cytokinins BAP, TDZ and Kn for its effectiveness to induce the differentiation of somatic embryos. The best response was observed in protocorms cultured half strength MS medium supplemented with 2,4-D at 3.39 μM and TDZ at 6.80 μM. Both epidermal and sub epidermal cells were involved in the formation of embryos. The proembryos developed into globular stage and subsequently developed into protocoms. Complete plantlets were formed after 60 days of culture. The plantlets were acclimatized in plastic pots containing sterilized vermiculite. The survival rate was 76%.

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Section: Discussioncontrasting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Direct somatic embryogenesis of Malaxis densiflora (A. Rich.) Kuntze

Mahendran

Bai

2016

Journal of Genetic Engineering and Biotechnology

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“…Usually, the combination of physical stress with high auxin concentration in the culture medium improves the embryogenic response. This effect was observed in Cattleya maxim where the effect in the SE induction was evaluated using a combination of salt (0.3 M NaCl) or osmotic stress (sorbitol 0.4 M), and the culture in a medium supplemented with 2,4-D (0.45 μM) significantly increases the percentage of protocorms with embryogenic calli (Cueva Agila et al, 2015). In some angiosperms such as Panax ginseng , the treatment of somatic embryos with abscisic acid (ABA) and polyethylene glycol (PEG) at a concentration of 20 μM and 3.75%, respectively, improve both the maturation and regeneration of somatic embryos compared to the untreated (Langhansová et al, 2004).…”

Section: Stress and Somatic Embryogenesismentioning

confidence: 88%

Signaling Overview of Plant Somatic Embryogenesis

et al. 2019

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Somatic embryogenesis (SE) is a means by which plants can regenerate bipolar structures from a somatic cell. During the process of cell differentiation, the explant responds to endogenous stimuli, which trigger the induction of a signaling response and, consequently, modify the gene program of the cell. SE is probably the most studied plant regeneration model, but to date it is the least understood due to the unclear mechanisms that occur at a cellular level. In this review, the authors seek to emphasize the importance of signaling on plant SE, highlighting the interactions between the different plant growth regulators (PGR), mainly auxins, cytokinins (CKs), ethylene and abscisic acid (ABA), during the induction of SE. The role of signaling is examined from the start of cell differentiation through the early steps on the embryogenic pathway, as well as its relation to a plant’s tolerance of different types of stress. Furthermore, the role of genes encoded to transcription factors (TFs) during the embryogenic process such as the LEAFY COTYLEDON (LEC), WUSCHEL (WUS), BABY BOOM (BBM) and CLAVATA (CLV) genes, Arabinogalactan-proteins (AGPs), APETALA 2 (AP2) and epigenetic factors is discussed.

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“…Therefore, the sterilization process at high temperatures can cause the degradation of IAA levels. Several previous studies have also indicated that the administration of endogenous auxin, such as 2,4-D can modify the IAA balance by either decreasing or increasing IAA levels (Agila et al, 2015;De Castro Marcato et al, 2017;Xu et al, 2022). The decrease in IAA levels is assumed to be related to the ability of 2,4-D to stimulate the conjugation of endogenous IAA with aspartate, as well as a decrease in the YUC gene, which is significant in determining IAA levels (Islam, 2015;Takato et al, 2017).…”

Section: Percentage Of Callus Formationmentioning

confidence: 95%

Callus formation of Coffea canephora induced with 2,4 dichlorophenoxyacetic acid and tomato extract supplements

Fahira,

Latunra,

Johannes

2023

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Robusta coffee (Coffea canephora) is Indonesia's essential leading trade commodity. Robusta coffee is preferred for its bitter taste. However, robusta coffee productivity decreased due to bad agriculture practice. Tissue culture is an alternative to cultivation with various advantages. The plant growth regulators (PGRs) influence tissue culture’s success. Due to its stability, 2,4 dichlorophenoxyacetic acid has become a common synthetic PGRs. PGRs can be obtained from natural ingredients, such as tomatoes. This study aimed to analyze the effect of 2,4-dichlorophenoxyacetic acid and tomato extract addition and its appropriate concentration for callus induction of robusta coffee in vitro. This research was conducted at the Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University. This study used a Complete Randomized Design with two factors. The first factor was the concentration of tomato extract (0%; 7.5%; 10%; and 12.5%). Meanwhile, the second factor was the concentration of 2,4 dichlorophenoxyacetic acid (0 ppm; 1 ppm; 2 ppm; and 3 ppm). Observation parameters include the percentage of callus formation, callus growing time, callus fresh weight, callus color, and callus texture. The quantitative data were analyzed by the Kruskal-Wallis test followed by the Mann-Whitney Test to compare the effect of each treatment. The results showed that among other treatment combinations, treatment with the addition of 2 ppm 2,4-D and 10% tomato extract (T2D2) and treatment with the addition of 3 ppm 2,4-D and 10% tomato extract (T2D3) had the best effect in inducing the callus of robusta coffee.

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Combination of 2,4-D and stress improves indirect somatic embryogenesis inCattleya maximaLindl.

Cited by 13 publications

References 40 publications

Direct somatic embryogenesis of Malaxis densiflora (A. Rich.) Kuntze

Direct somatic embryogenesis of Malaxis densiflora (A. Rich.) Kuntze

Signaling Overview of Plant Somatic Embryogenesis

Callus formation of Coffea canephora induced with 2,4 dichlorophenoxyacetic acid and tomato extract supplements

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