Combination of two lipases more efficiently catalyzes methanolysis of soybean oil for biodiesel production in aqueous medium

Guan, Feifei; Pu, Peiyu; Wang, Guili; Yin, Tie; Peng, Qianqian; Huang, Jinjin; Guan, Guohua; Li, Ying

doi:10.1016/j.procbio.2010.06.021

Cited by 70 publications

(51 citation statements)

References 24 publications

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“…The amount of intermediates accumulated significantly decreases when a mono-and diacylglycerol lipase from A. oryzae is mixed with 1,3-regiospecific triacylglycerol lipase (Hama et al, 2009). Another combination of two lipases (Rhizomucor miehei lipase and Penicillium cyclopium lipase) was also attempted (Guan et al, 2010). Adachi et al (2011) developed an immobilized recombinant A. oryzae coexpressing triglyceride lipase and partial glyceride lipase that attains a methyl ester yield of 98% with low contents of residual glycerides.…”

Section: Combination Of Lipasesmentioning

confidence: 99%

Enzymatic biodiesel production: An overview of potential feedstocks and process development

Hama¹,

Kondo

2013

Bioresource Technology

187

101

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Section: Combination Of Lipasesmentioning

confidence: 99%

Enzymatic biodiesel production: An overview of potential feedstocks and process development

Hama¹,

Kondo

2013

Bioresource Technology

187

101

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“…8 The initial rates observed in the reactions with high acid content (AV = 50 and 90) were higher than those obtained with AV = 8.5 using Lipozyme RM IM and Novozym 435 (Figures 4a,b) and Figures 5a,b). Li et al 9 also observed that the initial reaction rate of methanolysis of waste oil (AV = 135) catalyzed by mix of Lipozyme TL IM (3%) with Novozym 435 (1%) was faster than that of the methanolysis of refined oil (AV = 3.5) due to the high FFAs content.…”

Section: Biodiesel Synthesis Using Mixtures Of Commercial Immobilizedmentioning

confidence: 73%

“…There are several reports in which mixtures of different types of lipases were used successfully to catalyze transesterification and interesterification reactions. 4,[6][7][8][9][10] About 75% of the biodiesel produced in Brazil is derived from soybean oil. However, in Brazil, there are other sources of vegetable oils, like Jatropha curcas, Acrocomia aculeate, and palm oils that are interesting for biodiesel production, as they have higher productivity compared with soybean oil.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Biodiesel Synthesis From Acid Oil Using a Lipase Mixture

Pedro¹,

Parreira²,

Correia³

et al. 2017

Quim. Nova

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The conventional biodiesel production process has some disadvantages. It is necessary to use refined vegetable oils with low free fatty acids (FFAs) content. An alternative route is to use low-cost acid oils in an enzymatic process. The use of lipases allows simultaneous esterification of FFAs and transesterification of triglycerides present in raw material forming alkyl esters. The aim of this work was to study the production of biodiesel using soybean oils with different acid contents (Acid Value of 8.5, 50, 90) and ethanol catalyzed by commercial immobilized lipases (Novozym 435, Lipozyme RM IM and Lipozyme TL IM). A significant decrease of acid value was observed mainly with Novozym 435 and Lipozyme RM IM. The use of a mixture of two immobilized lipases was also investigated to decrease catalyst cost and increase the amount of ester produced. The three commercial immobilized lipases were mixed in a dual system and tested for biodiesel synthesis from acid oil (AV of 8.5, 50 and 90). A positive synergistic effect occurred mainly for Lipozyme TL IM (1,3-specific lipase) and Novozym 435 (non-specific lipase) blend. The ester content doubled when this lipase mixture was used in ethanolysis of acid oil with AV of 90.

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“…Los aspectos comunes de estas investigaciones consisten en optimizar las condiciones de reacción (solvente, temperatura, pH, tipo de microorganismo que genera la enzima, etc) con el fin de establecer características adecuadas para una futura aplicación industrial. Sin embargo, los rendimientos y tiempos de reacción son aun desfavorables si se comparan con la transesterificación básica [34][35][36][37][38][39][40][41].…”

Section: Transesterificación Catalizada Por Lipasas Enzimáticasunclassified

El aceite de palma africana elae guineensis: Alternativa de recurso energético para la producción de biodiesel en Colombia y su impacto ambiental

2014

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El aceite de palma africana elae guineensis: Alternativa de recurso energético para la producción de biodiesel en Colombia... El aceite de palma africana RESUMENEn Colombia, la contaminación atmosférica generada durante la quema de combustibles fósiles, es uno de los principales problemas ambientales causante del deterioro de la calidad del aire, lo cual impacta negativamente al medio ambiente y la salud humana. Actualmente, la preocupación mundial por la protección del planeta, originó la necesidad de buscar alternativas energéticas en las fuentes renovables, para reducir las emisiones de gases efecto invernadero producidos durante la quema de los combustibles fósiles, que en parte son los causantes del calentamiento global. Los biocombustibles, definidos como combustibles de origen biológico obtenidos de manera renovable a partir de biomasa, son la alternativa propicia para cumplir con ese propósito. En Colombia, preocupados por la protección del medio ambiente, se ha promovido un incremento en la industria del biodiesel, biocombustible producido principalmente mediante la reacción de transesterificación básica del aceite extraído de la palma africana y condiciones adecuadas de agitación y temperatura. Este material producto de la revisión bibliográfica del estado del arte de la industria del biodiesel en Colombia, se presenta como una herramienta de consulta, en el cual se armonizan el tema de la química inmersa en el proceso de transesterificacion con factores de normatividad, política nacional e impacto ambiental.Palabras clave: Biocombustibles, Biodiesel, Biomasa, Transesterificación, Impacto ambiental, Palma Africana. ABSTRACTIn Colombia, the environmental contamination generated by the burning of fuels is the main cause for the deterioration of the air quality. This increase is the negative effects on the environment and the human health. Currently, the global concern to protect the planet, leeds to the need of finding energy alternatives obtained from renewable sources, which are less polluting and capable of reducing the emissions of greenhouse gases (produced during the process of fuel burning), which have an impact on global warming. Biofuels are defined as biological fuel obtained from biomass renewable way. They have become the favorable alternative for this purpose. In Colombia there has been an increase on the biodiesel industry (biofuel produced by basic transesterification reaction of the oil extracted from the African palm and appropriate conditions of stir and temperature) to protect the environment. This material has been produced as a consult tool, in which the chemical issue is harmonized from the transesterification reaction with factors related to national regulations and environmental impact.

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Combination of two lipases more efficiently catalyzes methanolysis of soybean oil for biodiesel production in aqueous medium

Cited by 70 publications

References 24 publications

Enzymatic biodiesel production: An overview of potential feedstocks and process development

Enzymatic biodiesel production: An overview of potential feedstocks and process development

Enzymatic Biodiesel Synthesis From Acid Oil Using a Lipase Mixture

El aceite de palma africana elae guineensis: Alternativa de recurso energético para la producción de biodiesel en Colombia y su impacto ambiental

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