Combinational effects of hexane insoluble fraction of Ficus septica Burm. F. and doxorubicin chemotherapy on T47D breast cancer cells

Abstract

Objective: To evaluate the effects of n-hexane insoluble fraction (HIF) of Ficus septica leaves in combination with doxorubicin on cytotoxicity, cell cycle and apoptosis induction of breast cancer T47D cell lines. Methods: The in vitro drugs-stimulated cytotoxic effects were determined using MTT assay. Analysis of cell cycle distribution was performed using flowcytometer and the data was analyzed using ModFit LT 3.0 program. Apoptosis assay was carried out by double staining method using ethydium bromide-acridin orange. The expression of cleaved-poly (ADPribose) polymerase (PARP) on T47D cell lines was identified using immunocytochemistry. Results: The combination exhibited higher inhibitory effect on cell growth than the single treatment of doxorubicin in T47D cells. In addition, combination of doxorubicin and HIF increased the incidence of cells undergoing apoptosis. HIF could improve doxorubicin cytotoxic effect by changing the accumulation of cell cycle phase from G 2 /M to G 1 phase. The combination also exhibited upregulation of cleaved-PARP in T47D cells. Conclusions: Based on this results, HIF is potential to be developed as co-chemotherapeutic agent for breast cancer by inducing apoptosis and cell cycle arrest. However, the molecular mechanism need to be explored further.

Tylophorine Abrogates G2/M Arrest Induced by Doxorubicine and Promotes Increased Apoptosis in T47D Breast Cancer Cells

“…This indicated that there is a synergistic effect of tylophorine and doxorubicin when these compounds are combined. In the previous study, combination of doxorubicin of Ficus septica alkaloid fraction which might contain tylophorine enhanced cytotoxic effects of doxorubicin synergistically through inducing apoptosis by increasing the expression of cleaved Poly (ADP-ribose) polymerase (cPARP) (Nugroho, 2012).…”

Intramedullary dermoid cyst with relatively atypical symptoms: a case report and review of the literature

“…The case series of epidermoid and dermoid tumors of the spinal cord presented by Bradford evidenced most of the cases with urinary symptoms, either retention or incontinence [11], however, the patient in our case denied any of these symptoms but rather presented with bowel disturbance. Gercek et al also reported the case of a patient with a spinal dermoid cyst with a dissynergic bladder [14]. …”

Cell Cycle Arrest Activity of Litsea Cubeba Lour: Heartwood and Fruit Extracts Against T47d Breast Cancer Cells

“…After incubation, the cells were shaken, and absorbance was measured using microplate reader at λ 595 nm. The data which were absorbed from each well were converted to percentage of viable cells [8,[10][11][12][13][14]. Research Article then incubated for 24 hrs.…”

“…The samples were analyzed using FACScan flow cytometer. Based on DNA content, percentage of cells in each of stage in cell cycle (G1, S, and G2/M) were calculated using ModFit Lt. 3.0.s [12,13,16].…”