Combined Biochemical and Serological Typing of Clinical Isolates of Klebsiella

Rennie, R. P.; Duncan, I. B. R.

doi:10.1128/aem.28.4.534-539.1974

Cited by 38 publications

(29 citation statements)

References 11 publications

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“…However, only a total of 39 serotypes occurred in common among K. planticola, K. oxytoca, and K. pneumoniae. Although there were reports that serotypes 2 and 21 strains occurred at frequencies significantly higher than did all other serotypes in the bacteremic isolates (4, 5), the majority of prior reports showed that no one serotype or serotypes were found to predominate in Klebsiella strains from various clinical sources (15,24,26). The results of the present study were in accord with this conclusion.…”

Section: K Serotype Distribution Among 439 Clinical Isolates Of Four supporting

confidence: 86%

Identification of Species and Capsular Types of Klebsiella Clinical Isolates, with Special Reference to Klebsiella planticola

Mori

Ohta

Agata

et al. 1989

Microbiology and Immunology

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In the 77 reference strains for Klebsiella K types, there are 17 strains (22.1%) of Klebsiella planticola, 6 strains (7.8%) of Klebsiella oxytoca, 1 strain (1.3%) of Klebsiella terrigena, and 53 strains (68.8%) of Klebsiella pneumoniae. The species K. planticola, which was originally isolated from botanical and aquatic environments and hence thus named, was also identified at high incidence (81 strains, 18.5%) among the 439 recent clinical isolates of Klebsiella species. Among these K. planticola strains of hospital origin, 52 (64%) were isolated from sputum, 17 (21%) from urine, and the remaining 12 (15%) from other sources. The capsular types of these isolates were determined by the gel precipitation reaction. Seventy of 81 K. planticola isolates (86.4%) were typable by antisera to Klebsiella reference strains for K types and the K types of the clinical isolates distributed to 35 kinds of K types. The proportion of typable strains among clinical isolates of K. planticola was very similar to those in K. pneumoniae (87.5%) and K. oxytoca (86.0%).

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Section: K Serotype Distribution Among 439 Clinical Isolates Of Four supporting

confidence: 86%

Identification of Species and Capsular Types of Klebsiella Clinical Isolates, with Special Reference to Klebsiella planticola

Mori

Ohta

Agata

et al. 1989

Microbiology and Immunology

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“…The end point was the reciprocal of the greatest dilution at which a positive reaction could still be clearly detected. Cross-reactions between several capsular types were eliminated by the titration, because the titer of a cross-reaction is lower than the homologous reaction (24,25).…”

Section: Methodsmentioning

confidence: 99%

Serum antibodies to klebsiella capsular polysaccharides in ankylosing spondylitis

Sahly

Podschun

Sass

et al. 1994

Arthritis & Rheumatism

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Objective. To measure antibodies to Klebsiella capsular polysaccharides in the sera of HLA-B27 positive patients with ankylosing spondylitis (AS), compared with HLA-B27 positive and HLA-B27 negative healthy control subjects.Methods. Antibodies were detected by means of an enzyme-linked immunosorbent assay specific for each of the 77 known Klebsiella serotypes.Results. Significantly elevated frequencies and titers of antibodies to capsular polysaccharides K26, K36, and K50 were detected in sera from AS patients, compared with controls.Conclusion. These results suggest the predominance of Klebsiella serotypes K26, K36, and K50 in patients with AS.

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“…Biotyping based on an extended panel of biochemical and culture tests is certainly the most practicable method of typing for smaller laboratories that are epidemiologically not optimally equipped. Biotyping can be carried out by using macrotube tests alone (100,202) or by combining a commercially available miniaturized system such as the API 20E system with additional macrotube tests (185,217). However, because of the large number of reactions to be tested and the often long cultivation times-up to 90 days for demonstration of gelatinase (228)-biotyping of Klebsiella spp.…”

Section: Biotypingmentioning

confidence: 99%

Klebsiellaspp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors

1998

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SUMMARY Bacteria belonging to the genus Klebsiella frequently cause human nosocomial infections. In particular, the medically most important Klebsiella species, Klebsiella pneumoniae, accounts for a significant proportion of hospital-acquired urinary tract infections, pneumonia, septicemias, and soft tissue infections. The principal pathogenic reservoirs for transmission of Klebsiella are the gastrointestinal tract and the hands of hospital personnel. Because of their ability to spread rapidly in the hospital environment, these bacteria tend to cause nosocomial outbreaks. Hospital outbreaks of multidrug-resistant Klebsiella spp., especially those in neonatal wards, are often caused by new types of strains, the so-called extended-spectrum-β-lactamase (ESBL) producers. The incidence of ESBL-producing strains among clinical Klebsiella isolates has been steadily increasing over the past years. The resulting limitations on the therapeutic options demand new measures for the management of Klebsiella hospital infections. While the different typing methods are useful epidemiological tools for infection control, recent findings about Klebsiella virulence factors have provided new insights into the pathogenic strategies of these bacteria. Klebsiella pathogenicity factors such as capsules or lipopolysaccharides are presently considered to be promising candidates for vaccination efforts that may serve as immunological infection control measures.

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Combined Biochemical and Serological Typing of Clinical Isolates of Klebsiella

Cited by 38 publications

References 11 publications

Identification of Species and Capsular Types of Klebsiella Clinical Isolates, with Special Reference to Klebsiella planticola

Identification of Species and Capsular Types of Klebsiella Clinical Isolates, with Special Reference to Klebsiella planticola

Serum antibodies to klebsiella capsular polysaccharides in ankylosing spondylitis

Klebsiellaspp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors

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