Combined effects of laser-ICG photothermotherapy and doxorubicin chemotherapy on ovarian cancer cells

Cancer Therapy Combining Modalities of Hyperthermia and Chemotherapy: in vitro Cellular Response after Rapid Heat Accumulation in the Cancer Cell

“…However, in our preliminary study, we observed different hyperthermia chemotherapy interaction with two types of hyperthermia delivered at similar thermal dose but at different heating rate [100]. The rapid rate photothermal treatment showed synergy to chemotherapy whereas a sub-additive effect was found using the slow rate incubator hyperthermia.…”

Multifunctional Nanoparticles in Cancer: in vitro Characterization, in vivo Distribution

“…IR820-PGMD NP and DOX-IR820-PGMD NP cytotoxicity in cancer cells were first studied and compared to free IR820 or free DOX plus free IR820 in MDR cancer cell lines (Dx5) and non-MDR cancer cell lines (MES-SA). Our previous studies showed that a higher cancer cell killing effect can be obtained if HT is applied with chemotherapy than by chemotherapy alone (55). However, in that study drug-loaded NPs were not used to deliver the HT.…”

“…One of the most important NIR dyes is indocyanine green (ICG), since it is FDA-approved for some medical applications and has been used extensively clinically with a very good toxicity profile. In the past few decades, it has also been studied for its potential use in photodynamic therapy, photothermal therapy and optical imaging (54)(55)(56).…”

“…For this purpose, a laser heating system was designed (55). The system includes a laser module (RLDH808-1200-5, Roithner Laserthchnik, Austria), a laser holder, a heated stage insert (WPI Heated Stage Insert, World Precision Instruments Inc, Sarasota) and a mobile stage with an extension arm.…”

“…Our previous paper and other groups' reports demonstrated that the rate of photothermal treatment might also be affecting HT outcome, since the cells are not able to initiate the protective mechanism by inducing heat shock protein expression which would help to reduce DNA damage (55,149). Although the laser/Dye/NP HT produced approximately 9 times less thermal dose than incubator HT, it still resulted in significantly higher cytotoxicity than incubator HT, thus confirming the importance of heating rate.…”