Combined liquid chromatography mass spectrometry. Part I. Coupling by means of a moving belt interface

Arpino, Patrick

doi:10.1002/mas.1280080103

Cited by 57 publications

(7 citation statements)

References 86 publications

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“…The measurement of redox ratios is gaining significant interest for different diseases with disrupted metabolism, including cancer [5][6][7][8][9][10][11], Alzheimer disease [12] and heart disease [13,14]. Mass spectrometry has been used to characterize FAD/NADH [15], but this measurement requires sample destruction. Optical imaging provides a method to monitor and characterize redox ratios in a noninvasive manner, but optical penetration is a major limitation.…”

Section: Introductionmentioning

confidence: 99%

Development of an optical fiber‐based redox monitoring system for tissue metabolism

Zhang

Sorvina

Morrison

et al. 2022

Journal of Biophotonics

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An optical redox ratio can potentially be used to report on the dynamics of cell and tissue metabolism and define altered metabolic conditions for different pathologies. While there are methods to measure the optical redox ratio, they are not particularly suited to real‐time in situ or in vivo analysis. Here, we have developed a fiber‐optic system to measure redox ratios in cells and tissues and two mathematical models to enable real‐time, in vivo redox measurements. The optical redox ratios in tissue explants are correlated directly with endogenous NADH/FAD fluorescence emissions. We apply the mathematical models to the two‐photon microscopy data and show consistent results. We also used our fiber‐optic system to measure redox in different tissues and show consistent results between the two models, hence demonstrating proof‐of‐principle. This innovative redox monitoring system will have practical applications for defining different metabolic disease states.

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Section: Introductionmentioning

confidence: 99%

Development of an optical fiber‐based redox monitoring system for tissue metabolism

Zhang

Sorvina

Morrison

et al. 2022

Journal of Biophotonics

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“…the end of the column to the ion source, and the mobile phase is removed during this transport (Games et al, 1981). Excellent reviews on the moving belt technique can be found in the papers of Arpino and of Abian (Arpino, 1989;Abian, 1999). Nowadays, the belt interface principle has largely been abandoned in favor of more universal interface types.…”

Section: Lc/ms In Forensic Toxicologymentioning

confidence: 99%

Liquid chromatography?mass spectrometry in forensic toxicology

Bocxlaer¹,

Clauwaert²,

Lambert³

et al. 2000

Mass Spectrom. Rev.

115

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Liquid chromatography—mass spectrometry has evolved from a topic of mainly research interest into a routinely usable tool in various application fields. With the advent of new ionization approaches, especially atmospheric pressure, the technique has established itself firmly in many areas of research. Although many applications prove that LC—MS is a valuable complementary analytical tool to GC—MS and has the potential to largely extend the application field of mass spectrometry to hitherto “MS‐phobic” molecules, we must recognize that the use of LC—MS in forensic toxicology remains relatively rare. This rarity is all the more surprising because forensic toxicologists find themselves often confronted with the daunting task of actually searching for evidence materials on a scientific basis without any indication of the direction in which to search. Through the years, mass spectrometry, mainly in the GC—MS form, has gained a leading role in the way such quandaries are tackled. The advent of robust, bioanalytically compatible combinations of liquid chromatographic separation with mass spectrometric detection really opens new perspectives in terms of mass spectrometric identification of difficult molecules (e.g., polar metabolites) or biopolymers with toxicological relevance, high throughput, and versatility. Of course, analytical toxicologists are generally mass spectrometry users rather than mass spectrometrists, and this difference certainly explains the slow start of LC—MS in this field. Nevertheless, some valuable applications have been published, and it seems that the introduction of the more universal atmospheric pressure ionization interfaces really has boosted interests. This review presents an overview of what has been realized in forensic toxicological LC—MS. After a short introduction into LC—MS interfacing operational characteristics (or limitations), it covers applications that range from illicit drugs to often abused prescription medicines and some natural poisons. As such, we hope it can act as an appetizer to those involved in forensic toxicology but still hesitating to invest in LC—MS. © 2000 John Wiley & Sons, Inc., Mass Spec Rev 19: 165–214, 2000

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“…Furthermore, the manner of disposition of the solvent (e.g. by drip- ping, a slide contact or a nebulizer), the cleaning of the belt and the deactivation of the belt surface are important (Arpino 1989). Schmidt et al (1982) reported a rotating disk as the interface between the HPLC column and a plasma desorption mass spectrometer, and several drugs, e.g.…”

Section: Important Parameters Of Lc-ms Interfacesmentioning

confidence: 99%

Liquid chromatography coupled with mass spectrometry in the analysis of alkaloids

Verpoorte

Niessen

1994

Phytochemical Analysis

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Combined liquid chromatography mass spectrometry. Part I. Coupling by means of a moving belt interface

Cited by 57 publications

References 86 publications

Development of an optical fiber‐based redox monitoring system for tissue metabolism

Development of an optical fiber‐based redox monitoring system for tissue metabolism

Liquid chromatography?mass spectrometry in forensic toxicology

Liquid chromatography coupled with mass spectrometry in the analysis of alkaloids

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