Combined plasma rich in growth factors and adipose-derived mesenchymal stem cells promotes the cutaneous wound healing in rabbits

Abstract

BackgroundThe use of Plasma Rich in Growth Factors (PRGF) and Adipose Derived Mesenchymal Stem Cells (ASCs) are today extensively studied in the field of regenerative medicine. In recent years, human and veterinary medicine prefer to avoid using traumatic techniques and choose low or non-invasive procedures. The objective of this study was to evaluate the efficacy of PRGF, ASCs and the combination of both in wound healing of full-thickness skin defects in rabbits. With this purpose, a total of 144 rabbits were used for this study. The animals were divided in three study groups of 48 rabbits each depending on the administered treatment: PRGF, ASCs, and PGRF+ASCs. Two wounds of 8 mm of diameter and separated from each other by 20 mm were created on the back of each rabbit: the first was treated with saline solution, and the second with the treatment assigned for each group. Macroscopic and microscopic evolution of wounds was assessed at 1, 2, 3, 5, 7 and 10 days post-surgery. With this aim, 8 animals from each treatment group and at each study time were euthanized to collect wounds for histopathological study.ResultsWounds treated with PRGF, ASCs and PRGF+ASCs showed significant higher wound healing and epithelialization rates, more natural aesthetic appearance, significant lower inflammatory response, significant higher collagen deposition and angiogenesis compared with control wounds. The combined treatment PRGF+ASCs showed a significant faster cutaneous wound healing process.ConclusionsThe combined treatment PRGF+ASCs showed the best results, suggesting this is the best choice to enhance wound healing and improve aesthetic results in acute wounds.

Histological and ultrastructural evaluation of the effect of Platelet Rich Plasma (PRP) on wound healing in the tongue of normal and Streptozotocin-induced diabetic albino rats

“…This also agreed with our observations. Furthermore, MT staining in the study by (Chicharro et al, 2018) [8] came in agreement with our results as it revealed a significant increase in blood vessel formation in PRGF-treated wounds at all studied days compared to control groups. PRGF enhanced collagen deposition at days 1, 2 and 5 compared to control groups.…”

Injectable Gel Form of a Decellularized Bladder Induces Adipose-Derived Stem Cell Differentiation into Smooth Muscle Cells In Vitro

“…The human samples were anonymized, and the experimental procedure was previously evaluated and accepted by the Regional Ethics Committee for Clinical Research with Medicines and Health Products following the Code of Practice 2014/01. Rabbit adipose tissue was collected as a surplus tissue from one single individual, a female New Zealand rabbit (8 months old), shortly before sacrifice in the previous study [ 52 ]. Both HADSC (human ADSC) and RADSC (Rabbit ADSC) were isolated, expanded and characterized as previously described [ 53 , 54 ].…”

Histological characteristics of Platelet-Rich Fibrin clots obtained under various modes of blood centrifugation

“…Плазму, збагачену тромбоцитами, виготовляють шляхом відбору зразка крові з різними антикоагулянтами та наступним одночи двоступеневим центрифугуванням, у результаті чого отримують очищену плазму, збагачену тромбоцитами, або плазму, збагачену лейкоцитами та тромбоцитами. Проте присутність антикоагулянтів і необхідність додавання різних речовин як активаторів тромбоцитів можуть викликати негативні реакції організму як неаутологічні продукти (Franklin et al, 2017;Chicharro et al, 2018;Etulain, 2018;Maia et al, 2019). З огляду на це, до переваг PRF зараховують нескладну та швидку техніку його одержання, відсутність необхідності додавання сторонніх речовин, біохімічних обробок, імунних реакцій, передачі інфекційних захворювань, а також поступовий екстрацелю-лярний вихід із тромбоцитів факторів росту протягом семи діб (Kiran et al, 2011;Ayswaria et al, 2018).…”