Combined System for the Simultaneous Optical and Electrochemical Monitoring of Intra- and Extracellular NO Produced by Glioblastoma Cells

Pereira-Rodrigues, Nazaré; Zurgil, Naomi; Chang, Shan-Chwen; Henderson,; Bedioui, Féthi; Cj, McNeil; Deutsch, Mordechai

doi:10.1021/ac0483163

Cited by 24 publications

(15 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Methods for electrochemical detection of NO have recently become available (Wink et al, 1995;Christodoulou et al, 1996;Allen et al, 2000;Brunet et al, 2003;Zhang, 2004;Nunemaker et al, 2007), enabling measurements of NO on the second and sub-second time scales in local regions of neural circuitry in vivo (Buerk et al, 1996;Buerk et al, 2003a;Buerk et al, 2003b), in brain slices in vitro (Leonard et al, 2001;Ledo et al, 2002;Ferreira et al, 2005) and in neuronal cell cultures (Oni et al, 2004;Xu et al, 2004;Pereira-Rodriques et al, 2005). Here, we show for the first time that NO-selective microelectrodes can be used to measure NO production in the living mouse olfactory bulb.…”

Section: Introductionmentioning

confidence: 82%

Tonic and stimulus-evoked nitric oxide production in the mouse olfactory bulb

Lowe

Buerk

et al. 2008

Neuroscience

View full text Add to dashboard Cite

Nitric oxide (NO) has been long assumed to play a key role in mammalian olfaction. This was based largely on circumstantial evidence, i.e. prominent staining for nitric oxide synthase (NOS) and cyclic GMP or soluble guanylyl cyclase, an effector enzyme activated by NO, in local interneurons of the olfactory bulb. Here we employ innovative custom-fabricated NO micro-sensors to obtain the first direct, time-resolved measurements of NO signaling in the olfactory bulb. In 400 μm thick mouse olfactory bulb slices, we detected a steady average basal level of 87 nM NO in the extracellular space of mitral or granule cell layers. This NO 'tone' was sensitive to NOS substrate manipulation (200 μM L-arginine, 2 mM L-NAME) and Mg 2+ modulation of NMDA receptor conductance. Electrical stimulation of olfactory nerve fibers evoked transient (peak at 10 s) increments in NO levels 90 -100 nM above baseline. In the anesthetized mouse, NO micro-sensors inserted into the granule cell layer detected NO transients averaging 55 nM in amplitude and peaking at 3.4 sec after onset of a 5 sec odorant stimulation. These findings suggest dual roles for NO signaling in the olfactory bulbtonic inhibitory control of principal neurons, and regulation of circuit dynamics during odor information processing.

show abstract

Section: Introductionmentioning

confidence: 82%

Tonic and stimulus-evoked nitric oxide production in the mouse olfactory bulb

Lowe

Buerk

et al. 2008

Neuroscience

View full text Add to dashboard Cite

show abstract

“…The first is monitoring reactive oxygen and reactive nitrogen species dynamics (i.e., oxidative stress events (75)). The second is measuring neurotransmitter release, in particular, the release of the gaseous neuromodulators nitric oxide and carbon monoxide (76,77). The third application involves using a dMOCS in a whole-cell screening assay for monitoring oxygen dynamics.…”

Section: Discussionmentioning

confidence: 99%

Combined Imaging and Chemical Sensing with a Disposable Microscope Objective Chemical Sensor

Hartzell¹,

Danowski²,

Pantano³

2007

Applied Spectroscopy Reviews

View full text Add to dashboard Cite

This manuscript provides a concise review of the combined imaging and chemical sensing (CICS) technique performed with imaging fiber chemical sensors (IFCSs) and represents the first demonstration of CICS using a disposable microscope objective chemical sensor (dMOCS). The dMOCS assembly comprises two pieces that clamp around an imaging system's objective and a third adjoining piece that positions a sensing layer onto a sample's surface and within the objective's field of view. The prototype layer was oxygen-sensitive and was fabricated using a luminescent ruthenium metal complex and a gas permeable polymeric membrane. O 2 -sensitive dMOCSs were characterized with respect to their imaging capabilities, sensitivity, and temporal response, and the feasibility of performing a high-content screening assay was demonstrated with preliminary measurements of oxygen dynamics from living cells. While IFCSs are a requisite for analyzing remote samples that cannot be brought to a microscope stage, for those samples that can, CICS with a dMOCS possesses advantages with respect to spatial resolution, ease of fabrication, cost, and viewing area.

show abstract

“…Finally, o-phenylenediamine (o-PD) thin film was electrochemically deposited by controlled potential electrolysis at 0.9 V vs. Ag/AgCl in 6.5 mM o-PD in PBS during 20 min. This outermost layer was aimed at enhancing the selectivity of the electrode against possible interfering molecules [14,15]. The thickness of the total coatings of the disc section of the sensor by the NO sensitive layers may be estimated to be less than 1 mm (depending on how the Nafion sticks onto the disc).…”

Section: Methodsmentioning

confidence: 99%

In Vivo Electrochemical Detection of Nitroglycerin‐Derived Nitric Oxide in Tumor‐Bearing Mice

Griveau¹,

Séguin²,

Scherman³

et al. 2009

Electroanalysis

Self Cite

View full text Add to dashboard Cite

The ability of an inserted electrode to detect in vivo NO in tumor was achieved by using a chemically modified Pt/Ir electrode as NO-sensor and nitroglycerin NTG as NO donor. Enzymatically induced release of NO from NTG was tracked in the tumor, upon intra peritoneal injection of NTG. This approach allowed in vivo monitoring of the bioavailability of NO and could be applied to the in vivo study of candidate anticancer drugs acting on the NO pathways.Keywords: Tumor, In vivo detection, Nitric oxide, Nitroglycerin, NO-sensor, Electrochemical detection DOI: 10.1002/elan.200804300 Tumor vasculature plays an essential role in tumor survival and development, leading to the concepts of antiangiogenic and antivascular therapy [1 -3]. While the antiangiogenic strategy targets the formation of tumor new vessels, the antivascular drugs aim at targeting the existing tumoral vasculature and at causing tumor endothelial cells death [4]. One of the principal actors in the antivascular approach appears to involve the action of nitric oxide (NO) [2 -6]. Thus, it is believed that this induced NO-release could be considered, at least partially, as an in vivo marker of the anticancer activity of this class of agents. Interest in elucidating the mechanism of action of several classes of anticancer agents and also in exploring the pathways of the induced-NO release provides an impetus for conceiving a well-designed approach to locally detect NO in tumors, in vivo. Measuring NO from liquid samples is now well documented [7] and the electrochemical detection systems are recognized as being the only reliable analytical method to determine NO local concentration in real time, without disturbing its metabolism and its associated regulatory pathways [8,9].We recently extended the use of the electroanalytical concept and adapt it to a very special and complex biological tissue such as tumors [10]. We have demonstrated that miniaturized electrochemical sensor can be used in vivo for probing electroactive species in tumor-bearing mice. This was first achieved by performing for the first time in vivo cyclic voltammetry of K 4 Fe(CN) 6 . In addition, the use of two NO donors allowed, for the first time, to demonstrate the feasibility of the electrochemical detection of directly injected exogenous NO in live tumor-bearing mice.Nitroglycerin (NTG) is one of the oldest NO donors reported in the literature. It is enzymatically metabolized in living systems and the produced NTG-derived NO diffuses into the surrounding tissues [11,12]. Thus, the change in basal level of NO through induced enzymatically decomposed NTG constitutes a pertinent approach to explore our electroanalytical methodology to footpath intra tumoral NO. This led us to explore the monitoring of changes in the intra tumoral NO levels upon intra-peritoneal injection of NTG. Thus, in this work we report for the first time on the local in vivo tracking of changes in level of NTG-derived NO in tumor bearing mice in order to path the way for future studies of the evaluation of ...

show abstract

Combined System for the Simultaneous Optical and Electrochemical Monitoring of Intra- and Extracellular NO Produced by Glioblastoma Cells

Cited by 24 publications

References 22 publications

Tonic and stimulus-evoked nitric oxide production in the mouse olfactory bulb

Tonic and stimulus-evoked nitric oxide production in the mouse olfactory bulb

Combined Imaging and Chemical Sensing with a Disposable Microscope Objective Chemical Sensor

In Vivo Electrochemical Detection of Nitroglycerin‐Derived Nitric Oxide in Tumor‐Bearing Mice

Contact Info

Product

Resources

About