Combining Trichoderma hamatum THSW13 and Pseudomonas aeruginosa BJ10–86: a synergistic chili pepper seed treatment for Phytophthora capsici infested soil

Abstract

Phytophthora capsici infection of chili pepper seedlings can cause substantial losses due to damping-off and collar rot diseases. Chemical control is no longer effective due to reported resistance development, on top of the related environmental concerns and the consumer demands for reduced use of fungicides. Biological control is a sustainable option, with several agents having been reported to be effective against this pathogen. This research focused on optimizing the application of strain THSW13 of Trichoderma hamatum and a bacterial isolate BJ10-86 with the objectives of improving chili pepper seed germination, reduce damping-off disease incidence, and improve the growth of the seedlings. Bacterial isolate BJ10-86 was subjected to molecular identification and found to be Pseudomonas aeruginosa. Chili pepper seeds treated with the biocontrol agents, individually or in combination, were seeded into commercial nursery media that had been pre-inoculated with P. capsici zoospores. Over a period of 35 days the chili pepper seed treatments significantly (P = 0.008) reduced the disease incidence of seedlings damping-off. Combined application of T. hamatum and P. aeruginosa was the best biocontrol treatment with an area under disease curve of only 36.61 units compared to 92.87 units for the control treatment. Similar results were observed in vitro where T. hamatum and P. aeruginosa synergistically inhibited P. capsici growth by 73.2 %. The inhibition activity of this treatment was similar to mefenoxam treatment, which implies that it is an effective and sustainable alternative for chili pepper seed treatment. The biocontrol seed treatment had no effect on seed germination and seedling growth.

Discovery of γ-Lactam Alkaloid Derivatives as Potential Fungicidal Agents Targeting Steroid Biosynthesis

“…Then, the cultures were flooded with double-distilled water and incubated under continuous light for 10 days to induce zoosporangia. To encourage the release of zoospores, they were transferred into a refrigerator at 4 °C for 30 min and then at room temperature for 30 min. , Then, the concentration of the zoospores was adjusted to 2 × 10 4 zoospore·mL –1 .…”

Antagonistic Potential of Native Trichoderma spp. against Phytophthora cinnamomi in the Control of Holm Oak Decline in Dehesas Ecosystems

“…Among the several Trichoderma spp. isolated in the soil rhizosphere of the selected trees and presenting high or very high MGI rates, we found some species already described as antagonistic organisms of P. cinnamomi, or other pathogenic Phytophthora spp., such as T. harzianum [24], T. hamatum [21,22], or T. virens [63]. However, others have not yet been described as potential biocontrol agents and presented very high rates of MGI including T. viridarium, T. gamsii, T. olivascens, T. linzhiense, or T. hirsutum.…”

“…To date, much research has confirmed the role of Trichoderma spp. In controlling or even reducing Phytophthorarelated diseases [21][22][23][24][25][26].…”

Pengendalian Penyakit dan Pemacuan Pertumbuhan Tanaman Tomat dengan Perlakuan Plant Growth Promoting Rhizobacteria dan Trichoderma hamatum THSW13

“…Salah satu aspek yang penting dalam pemanfaatan agens hayati adalah teknik aplikasi. Chemeltorit et al (2017) melaporkan adanya pengaruh sinergistik aplikasi T. hamatum THSW13 dalam kombinasinya dengan Pseudomonas aeruginosa terhadap penekanan kejadian damping off dan peningkatan pertumbuhan bibit cabai dibanding perlakuan tunggal masing-masing agens hayati tersebut. Informasi tentang teknik aplikasi agens hayati menjadi penting, selain untuk meningkatkan pengaruhnya terhadap tanaman, juga untuk optimasi biaya yang dikeluarkan.…”