Common Characteristics of Primary Cell Cultures Originated from Invasive Urothelial Carcinoma

A Cytologie, Ultrastructural and Immunocytochemical Comparison of Tumor Cells and Cell Cultures Originating in Invasive Bladder Carcinoma

“…The ultrastructural features of normal and malignant urothelial cells were the same as those of cultivated cells originating in these tissues, described in earlier reports [2][3][4]. …”

“…GSG sacs with membrane extensions, mem brane-bound vacuoles full of fibrillar material (green glos sy-patchy color by Papanicolaou) observed in cell cultures are also present in cell imprints from invasive bladder car cinoma. The observation that membrane extensions of the GSG sacs invade the cytoplasm of other cells as well as the entrance and exit of GSG through the cell membrane has indicated enzyme association with the GSG [2,3]. The fact that positive immunoreactivity for archymotrypsin is observed perinuclearly where the membranebound PAS-positive fibrillar material is ultrastructurally located, indicates that the fibrillar GSG material is associ ated with enzymes.…”

“…Of these recurrences, 20% will be deeply invasive or metastatic [1], An identification method of those low-Such cell cultures exhibited tumor nodules consisting of naked nuclei in a glycosaminoglycan (GSG) mass, intra cellular perinuclear and accumulated GSGs in sacs as well as membrane extensions [2,3]. These features were ob served in human embryonic cell cultures and were absent in normal urothelial cell cultures [4,5], This study aims to identify the observed cytologic fea tures of malignant bladder-cultivated cells in cell imprints and urine-exfoliated cells from patients with invasive bladder carcinoma.…”

Growth Characteristics of Cultured Human Normal Bladder Epithelial Cells: A Comparison with Urothelial Carcinoma Cell Cultures

“…In addition, established malignant cell lines are not considered to be representative of the orig inal tumor, since serial cell passage causes cellular changes and adaptation to in vitro conditions. The mor phologic characteristics of primary bladder carcinoma cell cultures have recently been reported [2,3], Tumor cell nodules, intracytoplasmic sac glycosaminoglycans and membrane extensions have been found to be com-…”