In the present study (N = 587), we collected data from the general population of Poland-a highly homogeneous country-to examine how religiosity and spirituality were related to prejudice toward the threatening out-groups of (a) refugees and (b) sexual minorities. The effects of religiosity and spirituality on prejudice were juxtaposed with the effects of national or communal narcissism. We found that religiosity was related to national narcissism and spirituality, yet the effects of religiosity on prejudice were independent of the effects of national narcissism. Despite refugees and sexual minorities representing different social groups, the relationships between religiosity, spirituality, and national narcissism with prejudice toward both these groups were similar. Our study suggests that psychological mechanisms involved in the link between religiosity, spirituality, and prejudice are distinct. One path is based on perceiving threats toward tradition and social norms (i.e., religiosity and national narcissism), while the second path is based on lower competitiveness (i.e., spirituality). Therefore, our study allowed us to explain how religiosity could be related to greater prejudice toward refugees and sexual minorities, revealing more similarities to national narcissism than spirituality.