Community emergence of CTX-M type extended-spectrum β-lactamases among urinary Escherichia coli from women

Ho, Pak‐Leung; Poon, Winnie W. N.; Loke, Shee-Loong; Leung, Marianne S.T.; Chow, Kin-Hung; Wong, Chi Fong; Yip, King-Sun; Lai, Eileen L.; Tsang, K. T.

doi:10.1093/jac/dkm144

Cited by 67 publications

(72 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although our study showed a high prevalence of the international clone ST131 (20,32) among phylogroup B2 bla CTX-M-15 -and bla CTX-M-14 -positive isolates, the genetic diversity of the 43 UTI isolates examined supported previous reports showing diverse populations of E. coli carrying bla CTX-M genes (12,15,29). The further molecular typing of the resistance plasmids from these isolates showed that the dissemination of bla CTX-M-14 and bla CTX-M-15 was associated mainly with IncI1 and IncF repB plasmids of widely different sizes, which confirmed that different IncF plasmids are the most common mediators of CTX-M enzymes (12,16).…”

Section: Discussionsupporting

confidence: 88%

Molecular Characterization and Antimicrobial Susceptibility Testing of Escherichia coli Isolates from Patients with Urinary Tract Infections in 20 Chinese Hospitals

Cao

Cavaco

et al. 2011

J Clin Microbiol

View full text Add to dashboard Cite

A total of 222 urinary Escherichia coli isolates from 20 tertiary hospitals in 15 different provinces and 4 municipalities in mainland China were characterized by antimicrobial susceptibility, phylogrouping, and the presence of plasmid-mediated quinolone resistance genes. A subset of 138 suspected extended-spectrum cephalosporinase (ESC) producers were examined for genes encoding cephalosporin resistance. Forty-three isolates harboring bla CTX-M-14 or bla CTX-M-15 were analyzed by pulsed-field gel electrophoresis (PFGE), and plasmids containing these genes were typed using PCR-based replicon typing (PBRT). Thirteen phylogroup B2 bla CTX-M-14 -and bla CTX-M-15 -positive isolates were analyzed by multilocus sequence typing (MLST). A frequent occurrence of resistance (>46%) was observed toward cephalosporins, gentamicin, and fluoroquinolones. Among the 222 isolates, 4 qnrS1, 4 qepA, and 16 aac(6)-Ib-cr genes were confirmed. Four major phylogroups (A, B1, B2, and D) and nontypeable isolates (NTs) were found among the isolates, with phylogroup D (54%) being the most common phylogroup. A total of 110 (80%) of the 138 screened isolates harbored bla CTX-M genes, with bla CTX-M-14 (71%) and bla CTX-M-15 (24%) being the most prevalent of these genes. Nine of the 13 CTX-M-15-or CTX-M-14-containing B2 isolates belonged to ST131. PFGE typing showed a high level of diversity, and plasmid analysis indicated a very large pool of different resistance plasmids mediating the spread of bla CTX-M genes in mainland China. An equally very high frequency of resistance and equally high levels of diversity in phylogroups, PFGE types, and plasmids were observed among community-and hospitalacquired E. coli isolates, indicating the presence of a large reservoir in the community and a long-term spread of cephalosporin resistance in China.

show abstract

Section: Discussionsupporting

confidence: 88%

Molecular Characterization and Antimicrobial Susceptibility Testing of Escherichia coli Isolates from Patients with Urinary Tract Infections in 20 Chinese Hospitals

Cao

Cavaco

et al. 2011

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…A total of 37 unrelated PFGE patterns were identified among the bla CTX-M -containing E. coli isolates, and 1 closely related or indistinguishable PFGE pattern was found in 4 of the CTX-M-positive isolates (Table 2). This high clonal diversity observed among our bla CTX-M -containing E. coli isolates is coincident with similar results obtained in other studies (Velasco et al, 2007;Hernández et al, 2005, Ho et al, 2007Briñas et al, 2005), indicating that the wide dissemination of the bla CTX-M genes among E. coli isolates is mainly due to the horizontal dissemination of resistance determinants and not to dissemination of resistant clones. Nevertheless, the clonal dissemination of CTX-M-15-producing E. coli isolates within the hospital and community has been reported, and very recently, the intercontinental emergence of E. coli clone O25:H4-ST131 producing CTX-M-15 has been reported (Nicolas-Chanoine et al, 2008).…”

Section: Jmm Correspondencesupporting

confidence: 92%

Characterization of extended-spectrum β-lactamases and integrons in Escherichia coli isolates in a Spanish hospital

Vinué

Lantero

Sáenz

et al. 2008

Journal of Medical Microbiology

View full text Add to dashboard Cite

“…Because all included patients were living in Phnom Penh, mediocre hygiene might have favored the diffusion of clones within an urban area, a phenomenon that had previously been observed in large-scale studies undertaken in the United Kingdom (12), Canada (36), Italy (37), and Brazil (15) but not in Hong Kong (13). In contrast to our results, clones identifi ed in those studies harbored the same β-lactamases.…”

Section: Discussioncontrasting

confidence: 81%

CTX-M β-Lactamases inEscherichia colifrom Community-acquired Urinary Tract Infections, Cambodia

Ruppé¹,

Hem²,

Lath³

et al. 2009

Emerg. Infect. Dis.

104

View full text Add to dashboard Cite

Despite the recent global spread of CTX-M β-lactamases in Escherichia coli isolates from community-acquired urinary tract infections (CA-UTIs), their dissemination has been little studied in developing countries. In a 2-year prospective study, we documented the prevalence of extended-spectrum β-lactamases (ESBLs) in E. coli that were responsible for CA-UTIs in Phnom-Penh, Cambodia. Ninety-three E. coli strains were included. We observed a high prevalence of resistance to amoxicillin (88.2% of strains), cotrimoxazole (75.3%), ciprofl oxacin (67.7%), gentamicin (42.5%), and third-generation cephalosporins (37.7%). A total of 34 strains carried ESBLs, all of which were CTX-M type. CTX-M carriage was associated with resistance to fl uoroquinolones and aminoglycosides. Using repetitive extragenic palindromic-PCR, we identifi ed 4 clusters containing 9, 8, 3, and 2 strains. The prevalence of CTX-M β-lactamases has reached a critical level in Cambodia, which highlights the need for study of their spread in developing countries.

show abstract

Community emergence of CTX-M type extended-spectrum β-lactamases among urinary Escherichia coli from women

Abstract: The study documented the community emergence of CTX-M as the predominant ESBL type among urinary isolates from women. The spread of CTX-M enzymes among isolates from young women is concerning and deserves close monitoring.

Cited by 67 publications

References 8 publications

Molecular Characterization and Antimicrobial Susceptibility Testing of Escherichia coli Isolates from Patients with Urinary Tract Infections in 20 Chinese Hospitals

Molecular Characterization and Antimicrobial Susceptibility Testing of Escherichia coli Isolates from Patients with Urinary Tract Infections in 20 Chinese Hospitals

Characterization of extended-spectrum β-lactamases and integrons in Escherichia coli isolates in a Spanish hospital

CTX-M β-Lactamases inEscherichia colifrom Community-acquired Urinary Tract Infections, Cambodia

Contact Info

Product

Resources

About