Background: Evaluation of dengue prevention and control program is important to describe the current situation, results and identying several drawbacks. This study aims to evaluatue dengue prevention and control program based on input, process and output Materials and Methods: This is qualitative study, located in Banyumas Regency, Central Java Indonesia. 38 of informants, consist of Banyumas Regency health officer, staff of primary health care, voluntary cadre and community were interviewed. To increase validity of this study, triangulation of source and method was carried out. Program evaluation consist of input (human resources, fund, method, regulation, and materials), process (plan, organization, implementation and evaluation), output (completeness of report). Result:. Results of this study indicated that the input component of dengue prevention and control program (funds, methods, and time) were relatively sufficient. While program implementation such as fogging focus, larvacide, mosquito breeding site eradication and regular larvae checking were still not optimal Conclusion:. This study suggested the need of supervision for program implementers to monitor the performance of Jumantik in conducting regular larvae checking. Result of this study also provide evaluation and feed back in each aspect of input, process and output, therefore improvemet in each aspect could be done effectively.