Compact Image Slicing Spectrometer (ISS) for hyperspectral fluorescence microscopy

Abstract

An image slicing spectrometer (ISS) for microscopy applications is presented. Its principle is based on the redirecting of image zones by specially organized thin mirrors within a custom fabricated component termed an image slicer. The demonstrated prototype can simultaneously acquire a 140nm spectral range within its 2D field of view from a single image. The spectral resolution of the system is 5.6nm. The FOV and spatial resolution of the ISS depend on the selected microscope objective and for the results presented is 45×45μm 2 and 0.45μm respectively. This proof-of-concept system can be easily improved in the future for higher (both spectral and spatial) resolution imaging. The system requires no scanning and minimal post data processing. In addition, the reflective nature of the image slicer and use of prisms for spectral dispersion make the system light efficient. Both of the above features are highly valuable for real time fluorescent-spectral imaging in biological and diagnostic applications.

Snapshot 3D optical coherence tomography system using image mapping spectrometry

“…can map 3D information (x,y,λ ) onto a 2D detector for acquisition in a single camera integration [25]. IMS provides a simple and direct approach for hyperspectral imaging, enabled by advances in large format detectors and the development of a component termed image mapper which consists of multiple facets of different 2D tilt angles.…”

“…We previously developed a snapshot hyperspectral imaging platform based on principles of imaging mapping/slicing spectrometery (IMS) [25]. Here, we report on the development and use of IMS to acquire a full 3D OCT volume in a single snapshot image capture.…”

High Throughput AOTF Hyperspectral Imager for Randomly Polarized Light

“…For applications with randomly polarized signals (e.g., fluorescence and diffuse reflectance), half of the signal maybe lost outright. Consequently, the throughputs of AOTF and LCTF are typically in the 40% [9] and 10% [25] range, respectively. This throughput is significantly lower than the 60-90% typically found in thin film band-pass filters [26], consequently limiting their use in low light applications [27].…”

A multi-object spectral imaging instrument

“…There exists a number of snapshot techniques for spectral imaging [9][10][11][12]. They have applications in astronomy and microscopy [13] and also in opthalmology [14,15].…”