Comparative analy-sis of glucose metabolism in strains of Vibrio cholerae, biovar El Tor

Zadnova, S. P.; Cheldyshova, N. B.; Крицкий, А. А.; Адамов, А. К.; Devdariani, Z. L.; Кутырев, Владимир

doi:10.18821/0208-0613-2017-35-2-64-69

Mol. genet. mikrobiol. virusol.

2017

DOI: 10.18821/0208-0613-2017-35-2-64-69

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Comparative analy-sis of glucose metabolism in strains of Vibrio cholerae, biovar El Tor

S. P. Zadnova¹,

N. B. Cheldyshova²,

А. А. Крицкий³

et al.

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Cited by 4 publications

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“…The molecular mechanism of this difference in glucose metabolism has been deduced by characterizing the function of 3 genes-VC1589 (alsD, acetolactate decarboxylase), VC1590 (alsS, acetolactate synthase), and VC1591 (alsO, oxidoreductase)-that regulte the neutral fermentation that produces 2,3-butanediol in El Tor biotype strains. The classical biotype strains are unable to generate neutral fermentation products, perhaps due to 2 point mutations in the regulatory gene VC1588 (alsR, a transcriptional regulator of the alpha-acetolactate operon) and 1 point mutation in VC1590 5,7 .…”

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Alterations in glucose metabolism in Vibrio cholerae serogroup O1 El Tor biotype strains

Lee

Kim

Baek

et al. 2020

Sci Rep

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The 2 biotypes of Vibrio cholerae O1 serogroup strains-classical and El Tor-use glucose in distinct ways. Classical biotype strains perform organic acid-producing fermentation and eventually lose viability due to the self-induced creation of an acidic environment, whereas El Tor biotype strains use an alternative neutral fermentation pathway, which confers them with survival advantages. However, we report that the neutral fermentation pathway has only been recruited in prototype Wave 1 El Tor biotype strains, which have not been isolated since the mid-1990s. Current Wave 2 and Wave 3 atypical El Tor strains contain a single-base deletion in a gene that directs bacteria toward neutral fermentation, resulting in the loss of neutral fermentation and an appearance that is similar to classical biotype strains. Moreover, when sufficient glucose was supplied, Wave 1 El Tor strains maintained their use of acid-producing fermentation, in parallel with neutral fermentation, and thus lost viability in the late stationary phase. The global replacement of Wave 1 El Tor strains by Wave 2 and 3 atypical El Tor strains implies that the acidic fermentation pathway may not be disadvantageous to V. cholerae. The characteristics that we have reported might improve oral rehydration in the treatment of cholera.

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“…Prototype El Tor biotype strains have not been isolated since the appearance of atypical El Tor strains 8 . In addition, many atypical El Tor strains have been shown to be negative or weakly positive in the Voges-Proskauer test, which implies that they lack neutral fermentation during glucose metabolism 7,9 .…”

mentioning

confidence: 99%

Alterations in glucose metabolism in Vibrio cholerae serogroup O1 El Tor biotype strains

Lee

Kim

Baek

et al. 2020

Sci Rep

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Identification of the Mechanisms of Acetoin Production Change in Genetically Altered Vibrio cholerae Strains O1 Biovar El Tor

Zadnova

Крицкий

Plekhanov

et al. 2019

Problemy Osobo Opasnykh Infektsii

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Objective of the study was to determine the mechanisms of acetoin biosynthesis change in genetically altered El Tor V. cholerae strains in Voges-Proskauer test.Materials and methods. We used nine genetically altered V. cholerae O1 strains, biovar El Tor, imported in the territory of the Russian Federation and Ukraine between 1993–2011, and four typical strains isolated in 1970–1972. When assessing acetoin production in V. cholerae strains in Voges-Proskauer test, the strain V. cholerae 569B O1 serogroup, classical biovar served as negative control of the assay. Relative gene expression was studied using real-time RT-PCR. Protein model construction was performed by means of automated server SWISS – MODEL.Results and discussion. It has been demonstrated that diagnostically significant feature – VogesProskauer reaction, utilized for V. cholerae O1 biovar differentiation, is changed in all investigated genetically altered strains of cholera agent, isolated in different periods of the current seventh pandemic (66.7 % of the strains show weakly positive reaction, 33.3 % – negative one). The data obtained testify to the reduction or absence of acetoin production in the investigated strains. Analysis of four structural genes of als operon, as well as expression of regulatory genes alsR and аphA, responsible for acetoin biosynthesis, has revealed that changes in acetoin production in the genovariants stem from the deletion of a single nucleotide (T in the position 315) in the structural gene alsD, encoding acetolaktat decarboxylase, and also from high levels of negative acetoin biosynthesis regulator expression – аphA gene. Modeling of the spatial (3-D) structure of AlsD protein in the genovariant M1293 and the reference-strain N16961 has shown that AlsD protein of the genovariant is, indeed, considerably reduced. However, spontaneous decarboxylation is possible in the absence of acetolaktat decarboxylase, which phenotypically manifests itself in borderline positive Voges-Proskauer test.

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Identification of <i>Vibrio cholerae</i> O1 strains of the El Tor biovar sensitive to polymyxin B and their molecular genetic analysis

Zadnova¹,

Krasnov²,

Plekhanov³

et al. 2021

Journal of microbiology, epidemiology and immunobiology

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The aim of the work was the identification and genetic characterization of Vibrio cholerae O1 strains of the El Tor biovar sensitive to polymyxin B among isolates imported to Russia.Materials and methods. We used 56 toxigenic and non-toxigenic strains of V. cholerae isolated from patients and from the environmental samples on the territory of Russia in 1970-2020. Resistance to polymyxin B was determined according to MR4.2.2218-07. The ability of strains to form a biofilm on the abiotic surface was assessed by a photometric method. Nucleotide sequences of genes were determined using UGENE 1.32 and MEGA X software. Phylogenetic analysis and tree construction were performed using "maximum parsimony" method in MEGA X software.Results and discussion. Two genetically modified strains of V. cholerae O1 biovar El Tor, M1509 and 3265/80, which were imported to Russia from India in 2012 and 2014, respectively, were identified. The analysis of 12 genes responsible for the resistance of V. cholerae O1 biovar El Tor strains to polymyxin B demonstrated that these strains contain the allele of the carRS gene, which is typical for all strains of cholera vibrio sensitive to polymyxin B. Study of V. cholerae M1509 and 3265/80 phylogeny based on SNP analysis showed that they fall into the same cluster with isolates containing the carRS allele isolated in India (2015) and Bangladesh (2018). V. cholerae M1509 and 3256/8 strains had the ability to form a biofilm similar to those observed in other genetically modified strains of cholera vibrio included into analysis.Conclusion. Highly virulent strains of the cholera agent with altered diagnostically significant features are imported into Russia, which should be taken into account when identifying V. cholerae O1 biovar El Tor strains isolated from patients and environmental samples during monitoring studies.

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Comparative analy-sis of glucose metabolism in strains of Vibrio cholerae, biovar El Tor

Cited by 4 publications

References 14 publications

Alterations in glucose metabolism in Vibrio cholerae serogroup O1 El Tor biotype strains

Alterations in glucose metabolism in Vibrio cholerae serogroup O1 El Tor biotype strains

Identification of the Mechanisms of Acetoin Production Change in Genetically Altered Vibrio cholerae Strains O1 Biovar El Tor

Identification of <i>Vibrio cholerae</i> O1 strains of the El Tor biovar sensitive to polymyxin B and their molecular genetic analysis

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