Comparative analysis of <i>Lacistema pubescens</i> and dexamethasone on topical treatment of skin inflammation in a chronic disease model and side effects

Silva, Josiane M da; Conegundes, Jéssica Leiras Mota; Pinto, Nícolas de Castro Campos; Mendes, Rita; Castañon, Maria Christina Marques Nogueira; Scio, Elita

doi:10.1111/jphp.12886

Cited by 9 publications

(20 citation statements)

References 23 publications

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“…Although the glucocorticoid‐induced adverse reactions on human skin usually occur after constant several topical applications, this work corroborates to previous studies which report that cutaneous atrophy in mice may take place after short‐term applications, [ 26,27 ] probably for the reason that mouse skin is thinner and much more permeable. [ 44 ] However, long‐term studies are needed to assure that INFLATIV is not susceptible to induce cutaneous atrophy or other side effects either on mice or human skin after longer periods of use.…”

Section: Resultssupporting

confidence: 90%

“…Other typical adverse reactions may occur, including hypopigmentation due to deficient melanin synthesis, stellate purpura, stretch marks, pseudo-scars, ulcerations, mucocutaneous infections, alterations in skin elasticity and mechanical properties, and epidermal barrier disturbance. [41] Although the glucocorticoid-induced adverse reactions on human skin usually occur after constant several topical applications, this work corroborates to previous studies which report that cutaneous atrophy in mice may take place after short-term applications, [26,27] probably for the reason that mouse skin is thinner and much more permeable. [44] However, long-term studies are needed to assure that INFLATIV is not susceptible to induce cutaneous atrophy or other side effects either on mice or human skin after longer periods of use.…”

Section: Cutaneous Atrophy Testsupporting

confidence: 89%

“…The difference between the values obtained from each measurement indicated the cutaneous atrophy degree. [ 27 ]…”

Section: Methodsmentioning

confidence: 99%

“…The difference between the values obtained from each measurement indicated the cutaneous atrophy degree. [27] Pharmaceutical stability tests Fifty grams of INFLATIV 6% and INFLATIV 12% was stored in clear glass bottles with a metal screw cap for pH and density analyses, and 5 g was used for organoleptic properties evaluation. The bottles were stored at room temperature, under refrigeration (4-8°C) or at 40°C AE 2°C and 75% AE 5% of relative air humidity for 3 months.…”

Section: Cutaneous Atrophy Testmentioning

confidence: 99%

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Preclinical studies indicate that INFLATIV, an herbal medicine cream containing Pereskia aculeata, presents potential to be marketed as a topical anti-inflammatory agent and as adjuvant in psoriasis therapy

Pinto

Maciel

Rezende

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives A previous study reported that the hexane fraction (HF) obtained from Pereskia aculeata leaves showed promising topical anti-inflammatory activity. Intending its future use in clinical practice, a herbal medicine cream named INFLATIV was developed. Its anti-inflammatory and antipsoriatic potential were investigated. INFLATIV was subjected to preliminary accelerated stability tests and to a degradation profile assessment. Methods INFLATIV was prepared at 6% and 12%. The anti-inflammatory activity was assessed by croton oil single and multiple application challenge in mice. Mouse tail test was used for antipsoriatic potential investigation. Cutaneous atrophy test was performed. Preliminary accelerated stability tests were performed together with a degradation profile by GC-MS analysis. Key findings The anti-inflammatory activity shown by INFLATIV was comparable to dexamethasone. However, the skin atrophy caused by that drug was not observed. INFLATIV modified skin parakeratotic differentiation into orthokeratosis, which revealed its antipsoriatic potential. The ingredients used were suitable to carry the bioactives as they were well permeated by the skin. The preliminary accelerated stability tests indicated that INFLATIV 6% is more stable than 12%. Conclusions The results demonstrated the relevant therapeutic and marketing potentials of INFLATIV, which is likely to be further evaluated in clinical trials for drug registration process with regulatory agencies.

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Section: Resultssupporting

confidence: 90%

Section: Cutaneous Atrophy Testsupporting

confidence: 89%

“…The difference between the values obtained from each measurement indicated the cutaneous atrophy degree. [ 27 ]…”

Section: Methodsmentioning

confidence: 99%

Section: Cutaneous Atrophy Testmentioning

confidence: 99%

See 2 more Smart Citations

Preclinical studies indicate that INFLATIV, an herbal medicine cream containing Pereskia aculeata, presents potential to be marketed as a topical anti-inflammatory agent and as adjuvant in psoriasis therapy

Pinto

Maciel

Rezende

et al. 2020

Journal of Pharmacy and Pharmacology

Self Cite

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“…Furthermore, it should be noticed that high concentrations of LGS (15 g/kg) significantly suppressed IL-6 release in vivo and in vitro , which was similar to DEX treatment. Nevertheless, DEX is an anti-inflammatory drug that has strong side effects such as alterations in body weight, atrophy of the adrenal and lymphoid organs (DaSilva et al, 2018), while LGS had no obvious toxicity in mice and patient in previous researches (Liu et al, 2016; Wang et al, 2011). MiRNAs usually bind to complementary sites in the 3-untranslated region (UTR) of target genes and regulate target gene expression by either translational inhibition, mRNA degradation, or both (Filipowicz et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Liang-Ge-San, a Classic Traditional Chinese Medicine Formula, Attenuates Lipopolysaccharide-Induced Acute Lung Injury Through Up-Regulating miR-21

Yang

Huo

et al. 2019

Front. Pharmacol.

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Background: Acute lung injury (ALI) is a life-threatening disease without effective chemotherapy at present. Liang-Ge-San (LGS) is a famous traditional Chinese medicine formula, which is used to treat ALI in China. However, only a few studies have addressed the mechanisms of LGS in ALI. Purpose: To evaluate the anti-inflammatory effects of LGS on lipopolysaccharide (LPS)-induced ALI, and to explore its underlying molecular mechanism. Methods: Murine RAW264.7 cells were treated with LGS and LPS (1 μg/ml). The generation of IL-6, TNF-α, IL-1β was detected by ELISA. The protein expressions of STAT3 and P-STAT3 (Tyr705) were determined by Western blotting and fluorescence confocal microscopy. STAT3 transcriptional activity was investigated by luciferase reporter gene assay. qPCR was used to detect the expressions of microRNA-21 (miR-21), STAT3, and IL-6. DSS cross-linking assay was used to assess the change of STAT3 dimer. In vivo anti-inflammatory effects of LGS were evaluated in an ALI mouse model induced by tracheal instillation of LPS (3 mg/kg). The anti-ALI effects were evaluated by ELISA, qPCR, Western blotting, BCA, and H&E assays. Results: LGS suppressed LPS-stimulated IL-6, TNF-α, and IL-1β generation in murine macrophages RAW264.7. Moreover, LGS down-regulated protein levels of P-STAT3 (Tyr705) and STAT3, inhibited STAT3 transcriptional activity, and up-regulated miR-21. Furthermore, blockage of miR-21 antagonized the inhibitory effects of LGS on the production of IL-6 and the expressions of P-STAT3 (Tyr705) and STAT3 as well as the formation of STAT3 dimer. Critically, LGS up-regulated the expression of miR-21 and inhibited the protein expressions of STAT3 and P-STAT3 (Tyr705) to reduce the release of IL-6 and inflammatory cell infiltration as well as the degree of edema in LPS-induced ALI mice. Conclusion: LGS inhibited LPS-induced ALI through up-regulating miR-21 and subsequently inhibiting the STAT3 signaling pathway, thereby decreasing the release of IL-6.

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The application of a lateral flow immunographic assay to rapidly test for dexamethasone in commercial facial masks

Wang

Guo

et al. 2019

Anal Bioanal Chem

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Dexamethasone (DE) is a synthetic glucocorticoid that is frequently added to cosmetic products for its good short-term effects, especially in facial masks, but long-term use is hazardous to the health. The abuse of DE in whitening and acne cosmetic products is currently a serious problem in China. It is necessary to establish a rapid method of detecting illegal DE addition in cosmetics. In the present study, a monoclonal antibody (mAb) against DE, 2D5-3D12, was developed that displayed cross-reactivities of 124.5%, 38.8%, 6.7%, 0.9%, 1.1%, 1.82%, and 2.39% with prednisolone, betamethasone, prednisone, beclomethasone, hydrocortisone, triamcinolone, and flumetasone, respectively. A colloidal gold-based lateral flow immunographic assay based on mAb 2D5-3D12 was established and used to determine the DE contents of commercial facial masks. The indicator range of the immunographic assay for DE was 100–200 ng/mL, and the results were consistent with those afforded by LC-MS. This novel method provides the advantages of simple sample treatment, a user-friendly procedure, and rapid detection. Graphical abstract Electronic supplementary material The online version of this article (10.1007/s00216-019-01948-2) contains supplementary material, which is available to authorized users.

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Comparative analysis of Lacistema pubescens and dexamethasone on topical treatment of skin inflammation in a chronic disease model and side effects

Abstract: The results suggested that ProHLP is an efficient and safer pharmaceutical formulation to treat chronic inflammatory diseases.

Cited by 9 publications

References 23 publications

Preclinical studies indicate that INFLATIV, an herbal medicine cream containing Pereskia aculeata, presents potential to be marketed as a topical anti-inflammatory agent and as adjuvant in psoriasis therapy

Preclinical studies indicate that INFLATIV, an herbal medicine cream containing Pereskia aculeata, presents potential to be marketed as a topical anti-inflammatory agent and as adjuvant in psoriasis therapy

Liang-Ge-San, a Classic Traditional Chinese Medicine Formula, Attenuates Lipopolysaccharide-Induced Acute Lung Injury Through Up-Regulating miR-21

The application of a lateral flow immunographic assay to rapidly test for dexamethasone in commercial facial masks

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