Comparative analysis of phenolic contents and total antioxidant capacity of Moringa oleifera Lam

Vyas, Swati; Kachhwaha, Sumita; Kothari, S.L.

doi:10.5530/pj.2015.1.5

Cited by 24 publications

(14 citation statements)

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“…However, Food and Nutrition Sciences in the present study we documented the total phenolic content of CT to be as high as 822.6 mg GAE/g in the methanolic fraction. Previous sequential extraction studies with soxhlet also reveal high phenolic content in the methanolic fraction from various parts of Moringa oleifera [46] and of Caesalpinia digyna root [47]. The fact that polyphenolic compounds in plants are mostly found in conjugated forms with glycosides, proteins and sugars which are soluble in the specific solvent system [16] [48] [49].…”

Section: Discussionmentioning

confidence: 99%

“…In some cases, the methanolic fraction revealed anti-oxidant activity similar to or even greater than the known anti-oxidants(Table 2). This is not surprising, as the methanolic fraction exclusively contained the polyphenol compounds and that total phenolic content and anti-oxidant activities of various plant extracts including the CT were reported to be in direct positive co-relationship[46] [52][53]. However, more or less the Food and Nutrition Sciences constant presentation of highest anti-oxidant activities in multiple assay methods, which involve different reaction mechanism, P H conditions and vary according to compound type (hydrophilic and lipophilic)[54] [55] by methanolic fraction indicates it to be a source of multiple phenolics and flavonoids of potent anti-oxidant activities.…”

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confidence: 84%

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Phytochemicals Screening, Phenolic Estimation and Evaluation for Anti-Oxidant, Anti-Inflammatory and Anti-Microbial Activities of Sequentially Soxhlet Extracted Coconut Testa

Ojha¹,

Roy²,

Das³

et al. 2019

FNS

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Background: In many coconut industries, the outer layer of thin brown skin of coconut kernel known as testa is peeled out as a byproduct. Despite the testa is rich in fat and plenty of polyphenolic compounds, it has been underutilized either as animal feed, serving as raw materials for bio-diesel production or discarded directly. Anticipating coconut testa (CT) as a natural source of multiple phyto-chemicals, its exploitation for the pharmacological activity or utilization as value added product is required which may reduce the disposal costs as well. Methods: Secondary metabolites from CT were extracted sequentially with different organic solvents based on polarity in the soxhlet apparatus followed by extraction with sterilized water. The crude dried extracts thus prepared were evaluated for qualitative screening of phytochemicals and quantitative estimation of total phenols, flavonoids and tannin content. Moreover, the antioxidant, anti-inflammatory and anti-microbial activities were also investigated. Results: Phytochemicals screening revealed the presence of polyphenolic compounds in methanolic fraction including phenols (822.60 ± 16.36 mg/g), flavonoids (103.30 ± 9.78 mg/g) and tannin (663.50 ± 19.26 mg/g), whereas non-phenolic compounds were present in other fractions. While methanolic fraction showed invariably the highest anti-oxidant activity in multiple assay methods, non-phenolic compounds in aqueous and chloroform fractions exhibited high anti-inflammatory activity. Antimicrobial activity was observed by both phenolic and non-phenolic compounds. Conclusion: The findings of the study reveal that CT is a rich source of various polyphenolic and non-phenolic natural antioxidants, anti-inflammatory and antimicrobial compounds. These findings are promising and form the basis to identify the number of active components and their characterization. IntroductionCoconut (Cocos nucifera L.) belongs to the family Arecaceae [Palmae] and the subfamily Cocoideae is an important monocotyledon plant widely grown in the tropic and sub-tropics [1]. Further, certain religious and traditional values of coconut in most parts of India and other South East Asian countries have fared the production and productivity of coconut in these countries much better than many other countries. The coconut palm is sometimes referred to as 'great nut of India' owing to the use of all of its parts in some way or the other in coconut-growing areas. Apart from the use of coconut water, the natural sterile liquid from the young immature coconuts as a beverage; the kernel is used as the source of food and oil. Further, the wastes such as shell or coir are also used as raw materials for many industries for the production of shell-activated carbon or fibers for car seats and other household materials [2] [3] [4]. With the increasing demand for coconut oil, preparation of desiccated coconut powder or other products such as coconut cream and milk powder, the thin brown skin of coconut kernel known as testa is peeled out and processed in many coconu...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 84%

Phytochemicals Screening, Phenolic Estimation and Evaluation for Anti-Oxidant, Anti-Inflammatory and Anti-Microbial Activities of Sequentially Soxhlet Extracted Coconut Testa

Ojha¹,

Roy²,

Das³

et al. 2019

FNS

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“…[16]. Parts of this plant such as leaves, roots, seed, bark, fruit, flowers and immature pods act as cardiac and circulatory stimulants and also possess anti-tumour, anti-pyretic, anti-epileptic, anti-inflammatory, antiulcer, antispasmodic, diuretic, antihypertensive cholesterol lowering, anti-oxidant, antidiabetic, hepatoprotective, anti-bacterial and antifungal activities [17][18][19][20][21], and are being employed for the treatment of different ailments in the indigenous system of medicine.…”

Section: Introductionmentioning

confidence: 99%

Green synthesis and characterisation of CuNPs: insights into their potential bioactivity

Saran

Vyas

Mathur³

et al. 2018

IET nanobiotechnol.

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The current investigation involves the green synthesis of copper nanoparticles (CuNPs) from an aqueous plant extract of Moringa oleifera Lam by two methods: (I) time-based approach and (II) heat treatment of aqueous solution. Prepared CuNPs were characterised via Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy and transmission EM. The study also reveals the potential bioactivity of the prepared CuNPs. In vitro anti-microbial efficiency of CuNPs was estimated against bacterial and fungal strains by the agar well diffusion method. Anti-oxidant capacity of CuNPs was determined using ferric reducing ability of plasma (FRAP), lipid peroxidation (LPO) and peroxidase assays, while the antiplatelet potential was determined by measuring two haemostatic parameters (PT & APTT assay). The minimum inhibitory concentration was observed at 60 µg/ml against Streptomyces griseus and Aspergillus niger when NPs were prepared by method II. CuNPs prepared by the method I showed higher FRAP and LPO activities, while increased POX activity was found in CuNPs prepared by method II. CuNPs prepared using method I also showed better anti-oxidant and antiplatelet potential. It was observed that M. oleifera-derived CuNPs exhibits strong anti-microbial, anti-oxidant and APTT potential. This indicates potential utilization of green synthesized NPs for various industrial and therapeutic strategies.

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“…The growing body of evidence of the effectiveness of Moringa oleifera (MO) as a wound healing agent has led to a significant increase in its uses. Different parts of this plant act as antihyperthyroidism agents,[ 11 ] natural coagulants,[ 12 ] and possess antitumor,[ 13 ] antiinflammatory,[ 14 ] antiulcer,[ 15 ] antispasmodic,[ 16 ] antihypertensive,[ 17 ] cholesterol-lowering,[ 18 19 ] antidiabetic, hepatoprotective, antibacterial, and antifungal activities. [ 20 ] This versatility encouraged us to explore the effect of the ethyl acetate (EtOAc) fraction of MO leaves on the different parameters of wound healing, including antibacterial activity, antioxidant activity, cell proliferation, and migration rate in an in vitro wound healing model.…”

Section: Introductionmentioning

confidence: 99%

Chemical composition of Moringa oleifera ethyl acetate fraction and its biological activity in diabetic human dermal fibroblasts

et al. 2017

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Background:Moringa oleifera (MO), commonly known as the drumstick tree, is used in folklore medicine for the treatment of skin disease.Objective:The objective of this study is to evaluate the ethyl acetate (EtOAc) fraction of MO leaves for in vitro antibacterial, antioxidant, and wound healing activities and conduct gas chromatography-mass spectrometry (GC-MS) analysis.Materials and Methods:Antibacterial activity was evaluated against six Gram-positive bacteria and 10 Gram-negative bacteria by disc diffusion method. Free radical scavenging activity was assessed by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) radical hydrogen peroxide scavenging and total phenolic content (TPC). Wound healing efficiency was studied using cell viability, proliferation, and scratch assays in diabetic human dermal fibroblast (HDF-D) cells.Results:The EtOAc fraction showed moderate activity against all bacterial strains tested, and the maximum inhibition zone was observed against Streptococcus pyogenes (30 mm in diameter). The fraction showed higher sensitivity to Gram-positive strains than Gram-negative strains. In the quantitative analysis of antioxidant content, the EtOAc fraction was found to have a TPC of 65.81 ± 0.01. The DPPH scavenging activity and the hydrogen peroxide assay were correlated with the TPC value, with IC50 values of 18.21 ± 0.06 and 59.22 ± 0.04, respectively. The wound healing experiment revealed a significant enhancement of cell proliferation and migration of HDF-D cells. GC-MS analysis confirmed the presence of 17 bioactive constituents that may be the principal factors in the significant antibacterial, antioxidant, and wound healing activity.Conclusion:The EtOAc fraction of MO leaves possesses remarkable wound healing properties, which can be attributed to the antibacterial and antioxidant activities of the fraction.SUMMARY Moringa oleifera (MO) leaf ethyl acetate (EtOAc) fraction possesses antibacterial activities toward Gram-positive bacteria such as Streptococcus pyogenes, Streptococcus faecalis, Bacillus subtilis, Bacillus cereus and Staphylococcus aureus, and Gram-negative bacteria such as Proteus mirabilis and Salmonella typhimuriumMO leaf EtOAc fraction contained the phenolic content of 65.81 ± 0.01 and flavonoid content of 37.1 ± 0.03, respectively. In addition, the fraction contained 17 bioactive constituents associated with the antibacterial, antioxidant, and wound healing properties that were identified using gas chromatography-mass spectrometry analysisMO leaf EtOAc fraction supports wound closure rate about 80% for treatments when compared with control group. Abbreviations used: MO: Moringa oleifera; EtOAc: Ethyl acetate; GC-MS: Gas Chromatography-Mass Spectrometry; HDF-D: Diabetic Human Dermal Fibroblast cells.

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Comparative analysis of phenolic contents and total antioxidant capacity of Moringa oleifera Lam

Cited by 24 publications

References 9 publications

Phytochemicals Screening, Phenolic Estimation and Evaluation for Anti-Oxidant, Anti-Inflammatory and Anti-Microbial Activities of Sequentially Soxhlet Extracted Coconut Testa

Phytochemicals Screening, Phenolic Estimation and Evaluation for Anti-Oxidant, Anti-Inflammatory and Anti-Microbial Activities of Sequentially Soxhlet Extracted Coconut Testa

Green synthesis and characterisation of CuNPs: insights into their potential bioactivity

Chemical composition of Moringa oleifera ethyl acetate fraction and its biological activity in diabetic human dermal fibroblasts

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