Comparative assessment of canine-origin Lactobacillus johnsonii CPN23 and dairy-origin Lactobacillus acidophillus NCDC 15 for nutrient digestibility, faecal fermentative metabolites and selected gut health indices in dogs

Abstract

The present experiment was undertaken to validate a probiotic of canine origin for its potential use in dogs. A total of fifteen adult female Labrador dogs were allocated to three equal groups and fed a basal diet without probiotic (control) or with probiotic of either canine (Lactobacillus johnsonii CPN23; cPRO) or dairy (L. acidophilus NCDC 15; dPRO) origin for 9 weeks. The digestibility of most macronutrients remained similar among the groups; however, fibre digestibility was improved (P = 0·034) in dogs receiving cPRO. The faecal fermentative metabolites ammonia (P < 0·05) and lactate (P = 0·094) were altered favourably, indicating a positive influence of both probiotics. Faecal concentrations of acetate, propionate and butyrate were increased (P < 0·01) in both probiotic groups. However, improvements were higher in cPRO v. dPRO. The delayed-type hypersensitivity reaction to intradermal inoculation of phytohaemagglutinin-P was higher (P = 0·053) in cPRO as compared with control. The antibody response to sheep erythrocytes was, however, similar across the three groups. Overall, in dogs, the canine-origin probiotic was superior when compared with the dairy-origin probiotic.

Study of faecal parameters and body condition in dogs with a diet supplemented with Lactobacillus acidophilus D2/CSL (CECT 4529)

“…With regards to the effect of the probiotic on faecal parameters, the FM and FS of the dogs receiving the Lactobacillus supplementation demonstrated a significant improvement, meaning that, even in healthy dogs with no gastrointestinal disorder, the addition of this supplement to the diet helps to maintain the optimal balance of their intestinal microbiota. The present data are in agreement with a study where faecal consistency was improved in dogs with the addition of Lactobacillus acidophilus strain DSM 13241 (Pascher et al 2008) and in another trial on dogs belonging to the Boxer breed only treated with Lactobacillus acidophilus D2/CSL (Marelli et al 2020), while no effect on FS was recorded on dogs with a diet supplemented with Lactobacillus acidophilus NCDC 15 (Kumar et al 2017).…”

The Fecal Microbiota of Dogs Switching to a Raw Diet Only Partially Converges to That of Wolves

“…In this study, Lactobacillus was one of the most dominant genera in dogs fed kibble diet, and its abundance was also significantly higher in dogs fed kibble diet compared with dogs fed raw diet for 28 days and wolves according to LEfSe analysis. Some stains of Lactobacillus such as Lactobacillus reuteri and Lactobacillus johnsonii have been proposed as potential probiotic ( Kumar et al, 2017 ). However, a lingering question is whether such probiotics would be effective in raw-fed dogs and by extension in all natural-fed carnivorous mammals since an increasing amount of studies point to a low natural abundance of pathogens that may thrive on carbohydrates (e.g., cheetahs) ( Becker et al, 2014 ), hence, reducing the relevance of Lactobacillaceae probiotics.…”

Analysis of microbial diversity and composition in small intestine during different development times in ducks

“…In this study, the genera Bacillus, Corynebacterium 1, Lactococcus, Sphingomonas , and Haliangium correlated moderately positively with the increases in bodyweight and the duodenal, jejunal, and ileal lengths and weights. Some species of Bacillus and Lactococcus are widely used as probiotics to improve the intestinal environment and increase animal production (Kumar et al, 2017 ; Oh et al, 2017 ; Wang et al, 2017 ). For example, B. subtilis significantly reduced the dextran sodium sulfate ( DSS )-induced colonic mucosal injury and inflammatory factors in mice and improved their levels of short-chain fatty acids (Zhang et al, 2016b ).…”