Comparative assessment of DNA analysis in effusions by lmage analysis and flow cytometry

Er, Banks; Cd, Jennings; S, Jacobs; Dd, Davey

doi:10.1002/dc.2840100116

Cited by 11 publications

(8 citation statements)

References 7 publications

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“…In the current study 18 of the 28 (64%) malignant effusions were aneuploid. All (14) benign pleural effusions were diploid, consistent with reports of others [1,14,23]. Based on the above mentioned results this type of DNA analysis had a sensitivity of 64% and a specificity of 100%.…”

Section: Discussionsupporting

confidence: 76%

DNA ploidy, S-phase, and Ki-67 antigen expression in the evaluation of cell content of pleural effusions

Sikora¹,

Dworacki²,

Zeromski³

1996

Lung

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Cells from pleural effusions (28 malignant and 14 nonmalignant with reactive mesothelial cells) were examined in parallel by means of conventional cytology and multiparametric flow cytometry. The latter included the evaluation of DNA ploidy, the calculation of DNA index (DI), the determination of S phase fraction (SPF), and cell proliferation associated with Ki-67 antigen expression. Propidium iodide was used for the DNA staining, and fluorescein-conjugated monoclonal mouse antibodies were applied to the human Ki-67 antigen staining. The specimens were analyzed by the Becton Dickinson (BD) FACScan apparatus. 10(4) events were collected, and thereafter the obtained data were analyzed by the software Lysis II and CellFit (BD) for DNA and Ki-67 data analysis, respectively. Out of the 28 cases examined, 18 (64%) of the malignant effusions were aneuploid. All of the benign effusions were typically diploid. The average SPF of benign, malignant diploid, and malignant aneuploid cases was 6, 6, and 30 respectively. The comparison between SPF and DI in malignant cases showed a significant correlation (p < 0.001). The average of Ki-67-positive cells that were benign, malignant diploid, and malignant aneuploid were 11, 19, and 35, respectively. There was a significant correlation between Ki-67 expression, SPF (p < 0.05), and the percent of the aneuploid cell population (p = 0.01). The sensitivity of conventional cytology, the determination of DNA ploidy and of Ki-67 expression, was 80, 64, and 92%, respectively; specificity was 100% for cytology and DNA ploidy and 46% for Ki-67 expression. These data show that Ki-67 antigen expression is a potentially useful adjunct to cytometric DNA content analysis, determination of SPF, and conventional cytology in the diagnosis of malignant pleural effusions.

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Section: Discussionsupporting

confidence: 76%

DNA ploidy, S-phase, and Ki-67 antigen expression in the evaluation of cell content of pleural effusions

Sikora¹,

Dworacki²,

Zeromski³

1996

Lung

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“…In conclusion, these results confirm data from literature and indicate that cytometric analysis of nuclear content is a useful marker for identification of malignant cells in equivocal effusions and can be used to increase the cytological sensitivity in doubtful mesothelial proliferations or combined with IHC for distinction between primary or secondary tumors of the serous membranes. 16,17…”

Section: Discussionmentioning

confidence: 99%

Image cytometry: An aid for cytological diagnosis of pleural effusions

2005

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The conventional cytology rate for identification of neoplastic cells in effusions is about 60%. The rate of diagnostically equivocal effusions in routine cytology is dependent on the volume of effusion examined, type of preparation and staining, experience of the examiner, and application of ancillary methods. The aim of our study was to confirm the role of image cytometry analysis (DNA-ploidy) on pleural effusions. In this retrospective study based on 42 available cases with a histological diagnosis, we have examined 13 reactive mesothelial proliferations and 29 cases of malignant tumors (adenocarcinoma [ACA] or mesothelioma). The smears collected were submitted to the image analysis following a-three step protocol (smears stained with the Papanicolaou method were destained and then restained with Feulgen staining and finally analysed using image analysis cytometry). The results have shown that nonmalignant cases (reactive mesothelial proliferation) were all diploid and in contrast all aneuploid cases corresponded to malignant tumors. Only three mesotheliomas showed a diploid profile. In conclusion, these results confirm data from literature and indicate that cytometric analysis of nuclear content is a useful marker for identification of malignant cells in equivocal effusions and can be used to increase the cytological sensitivity in doubtful mesothelial proliferations.

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“…3 5-7 These include immunological methods, ligand techniques, and flow and static DNA cytometry. [8][9][10] Without doubt, specific staining techniques such as immunohistochemistry and flow cytometry must be performed in the primary diagnostic laboratory, whereas images can easily be sent to any specialised department by electronic mail, and the image information can be extracted by specialists from these transmitted images.…”

mentioning

confidence: 99%

Routine DNA cytometry of benign and malignant pleural effusions by means of the remote quantitation server Euroquant: a prospective study

Kayser¹

2000

J Clin Pathol

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Aim-To analyse the practicability and potential assistance of static DNA cytometry performed by means of the remote quantitation server Euroquant and the internet in routine diagnostic analysis of pleural eVusions, and to outline the role of DNA cytometry on pleural eVusions in distinguishing between benign and malignant (and herein primary versus metastatic) eVusions. Materials and methods-Cytological smears of 294 pleural eVusions were stained with the Feulgen method. The DNA content of a minimum of 300 randomly chosen analysis nuclei and 30 reference nuclei (lymphocytes) was measured by internet connection to the remote quantitation server Euroquant. Cytometric features were derived from the histograms, and the time needed for case evaluation, the reliability of staining and measurement procedures, and the contribution to the final diagnosis were evaluated. Results-Only 120 of 294 pleural eVusions could be measured. The total measurement time for each specimen was 60 minutes. The guidelines of the consensus report on DNA measurements were fulfilled. Seventy eight malignant (18 mesotheliomas, 60 metastatic tumours) and 42 benign eVusions were measured. Seven of 78 malignant eVusions were euploid and none of 42 benign eVusions were aneuploid. The sensitivity and specificity were 91% and 100%, respectively, for distinguishing benign from malignant eVusions, and 95% and 100%, respectively, for discriminating between benign and malignant eVusions caused by metastatic malignant tumours. Conclusions-StaticDNA cytometry using the remote quantitation server Euroquant can be performed reliably in the routine diagnosis of pleural eVusions; however, only 40% of eVusions meet the technical requirements for static DNA cytometry. Within the measurable cases, static DNA cytometry made an important contribution to the confirmation/ exclusion of malignancy. (J Clin Pathol 2000;53:760-764)

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Comparative assessment of DNA analysis in effusions by lmage analysis and flow cytometry

Cited by 11 publications

References 7 publications

DNA ploidy, S-phase, and Ki-67 antigen expression in the evaluation of cell content of pleural effusions

DNA ploidy, S-phase, and Ki-67 antigen expression in the evaluation of cell content of pleural effusions

Image cytometry: An aid for cytological diagnosis of pleural effusions

Routine DNA cytometry of benign and malignant pleural effusions by means of the remote quantitation server Euroquant: a prospective study

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