Comparative cell adhesion properties of cysteine extended peptide architectures

Söylemez, Saniye; Demir, Bilal; Oyman-Eyrilmez, Gizem; Kesici, Seçkin; Saylam, Aytül; Demirkol, Dilek Odaci; Özçubukçu, Salih; Timur, Suna; Toppare, Levent

doi:10.1039/c5ra23352f

Cited by 6 publications

(7 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 64 The peak at 286.3 eV could be assigned to the carbon atoms of the C=O or C–N 65 , 66 or to the O=C—N. 64 , 67 The amide related peak at 288.08 eV, 67 , 68 observed only for the functionalized sample, confirms the presence of peptides.…”

Section: Resultsmentioning

confidence: 85%

Short Ligand, Cysteine-Modified Warnericin RK Antimicrobial Peptides Favor Highly Sensitive Detection of Legionella pneumophila

et al. 2021

View full text Add to dashboard Cite

Culture-based methods for the detection of Legionella pneumophila are prohibitively slow and frequently inadequate. The problem has been addressed with biosensing technology that employs a variety of ligands for the specific capture of bacteria. However, the limited success of the application of mammalian antibodies, aptamers, and nucleic acid-based probes for sensitive biosensing has generated growing interest in exploring alternative biosensing architectures, such as those based on antimicrobial peptides (AMP) that are known for their attractive therapeutic applications. We report on the successful employment of cysteinemodified warnericin RK AMP for the operation of a highly sensitive biosensor of L. pneumophila based on digital photocorrosion of GaAs/AlGaAs nanoheterostructures. The replacement of the relatively cumbersome procedure commonly applied for the attachment of antibodies to COOH-terminated mercaptohexadecanoic acid self-assembled monolayers has allowed for a significant reduction in the distance at which bacteria are immobilized above the biosensor surface. An important consequence of this approach is the attractive limit of detection of L. pneumophila estimated at 2 × 10 2 CFU/mL. The target bacteria were captured four times more efficiently than P. fluorescens, B. subtilis, and E. coli, which is highly promising for environmental monitoring.

show abstract

Section: Resultsmentioning

confidence: 85%

Short Ligand, Cysteine-Modified Warnericin RK Antimicrobial Peptides Favor Highly Sensitive Detection of Legionella pneumophila

et al. 2021

View full text Add to dashboard Cite

show abstract

“…1−4 Various site-selective and/or chemoselective modifications of different amino acids of proteins were reported, including cysteine, 5−8 methionine, 9−12 lysine, 13−16 tyrosine, 17 tryptophan, 18 arginine, 19 etc. Thanks to thiol's high nucleophilicity, cysteine is widely utilized for selective modifications for various applications, including activity-based protein profiling (ABPP), 20 cell imaging, 21 covalent inhibitors, 22−25 and so on.…”

mentioning

confidence: 99%

“…Post translational modifications (PTMs) of proteins play key roles for diversified protein functions, which also creates interest in designing various synthetic methods to modify proteins. − Various site-selective and/or chemoselective modifications of different amino acids of proteins were reported, including cysteine, − methionine, − lysine, − tyrosine, tryptophan, arginine, etc. Thanks to thiol’s high nucleophilicity, cysteine is widely utilized for selective modifications for various applications, including activity-based protein profiling (ABPP), cell imaging, covalent inhibitors, − and so on.…”

mentioning

confidence: 99%

A Sulfonium Triggered Thiol-yne Reaction for Cysteine Modification

Hou

Wang

et al. 2020

J. Org. Chem.

View full text Add to dashboard Cite

We report a facile thiol-yne type reaction triggered by the sulfonium center. After facile propargylation of thiolethers, the resulting sulfonium could undergo facile addition with thiols in aqueous media at ambient temperature. Further applying this reaction in unprotected peptides bearing neighboring methionine and cysteine could enable a facile intramolecular addition to construct cyclic peptides with better stability, good glutathione resistance, and increased cellular uptakes. Also, the propargylated sulfonium may be used as robust and versatile probes to target cysteines containing biomolecules.

show abstract

“…Before cancer prevention and treatment, detecting cancer cells at an early stage by sensing their presence in the human body is essential [ 149 ]. In vitro cancer-cell detection based on the electrochemical method—which provides label-free, non-invasive, and non-destructive performance that could further support anticancer drug discovery—has emerged recently [ 120 , 139 , 140 , 141 , 142 , 143 , 150 , 151 ]. Angeline et al (2020) reported the electrical signal enhancement of stomach cancer cell (MKN-28) viability through the electrochemical detection method, which is then useful for drug screening applications [ 144 ].…”

Section: Electrochemical Biosensing For Highly Proliferative Cellsmentioning

confidence: 99%

Recent Advances in Electrochemical Sensors for the Detection of Biomolecules and Whole Cells

2020

View full text Add to dashboard Cite

Electrochemical sensors are considered an auspicious tool to detect biomolecules (e.g., DNA, proteins, and lipids), which are valuable sources for the early diagnosis of diseases and disorders. Advances in electrochemical sensing platforms have enabled the development of a new type of biosensor, enabling label-free, non-destructive detection of viability, function, and the genetic signature of whole cells. Numerous studies have attempted to enhance both the sensitivity and selectivity of electrochemical sensors, which are the most critical parameters for assessing sensor performance. Various nanomaterials, including metal nanoparticles, carbon nanotubes, graphene and its derivatives, and metal oxide nanoparticles, have been used to improve the electrical conductivity and electrocatalytic properties of working electrodes, increasing sensor sensitivity. Further modifications have been implemented to advance sensor platform selectivity and biocompatibility using biomaterials such as antibodies, aptamers, extracellular matrix (ECM) proteins, and peptide composites. This paper summarizes recent electrochemical sensors designed to detect target biomolecules and animal cells (cancer cells and stem cells). We hope that this review will inspire researchers to increase their efforts to accelerate biosensor progress—enabling a prosperous future in regenerative medicine and the biomedical industry.

show abstract

Comparative cell adhesion properties of cysteine extended peptide architectures

Abstract: This study presents the comparative cell attachment investigation of TAT and well-known RGD peptide modified surfaces.

Cited by 6 publications

References 44 publications

Short Ligand, Cysteine-Modified Warnericin RK Antimicrobial Peptides Favor Highly Sensitive Detection of Legionella pneumophila

Short Ligand, Cysteine-Modified Warnericin RK Antimicrobial Peptides Favor Highly Sensitive Detection of Legionella pneumophila

A Sulfonium Triggered Thiol-yne Reaction for Cysteine Modification

Recent Advances in Electrochemical Sensors for the Detection of Biomolecules and Whole Cells

Contact Info

Product

Resources

About