Comparative characteristic study from bone marrow-derived mesenchymal stem cells

Purwaningrum, Medania; Jamilah, Nabila Syarifah; Purbantoro, Steven Dwi; Sawangmake, Chenphop; Nantavisai, Sirirat

doi:10.4142/jvs.2021.22.e74

Cited by 32 publications

(17 citation statements)

References 107 publications

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“…The isolated cells expressed pluripotent stem cell markers and proliferative marker similar to previous report ( Sawangmake et al, 2014b). The markers have also been reported to be expressed in different origin of MSCs, such as bone marrow and dental pulp, from different species (Nantavisai et al, 2019(Nantavisai et al, , 2020Purwaningrum et al, 2021). Moreover, it is supported by the self-renewal and proliferative capabilities the cells possessed (Aponte and Caicedo, 2017).…”

Section: Discussionsupporting

confidence: 81%

Osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cells

Purbantoro

Osathanon

Nantavisai

et al. 2022

Heliyon

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Section: Discussionsupporting

confidence: 81%

Osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cells

Purbantoro

Osathanon

Nantavisai

et al. 2022

Heliyon

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“…MDS is a disease in which abnormalities are present in hematopoietic stem cells and the bone marrow microenvironment ( 23 ). BM-MSCs are an essential constituent of the bone marrow microenvironment and possess self-renewal and pluripotent potential ( 24 ).…”

Section: Discussionmentioning

confidence: 99%

Relationship of HIF‑1α expression with apoptosis and cell cycle in bone marrow mesenchymal stem cells from patients with myelodysplastic syndrome

Han

Zhao

et al. 2022

Mol Med Rep

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Myelodysplastic syndrome (MDS) is a group of abnormal clonal disorders with ineffective hematopoiesis, which are incurable with conventional therapy. Of note, MDS features an abnormal bone marrow microenvironment, which is related to its incidence. The hypoxia-inducible factor-1α (HIF-1α) transcriptional signature is generally activated in bone marrow stem/progenitor cells of patients with MDS. To analyze the expression of HIF-1α in bone marrow mesenchymal stem cells (BM-MSCs) and the apoptosis and cell cycle features associated with the disease, BM-MSCs were obtained from 40 patients with a definitive diagnosis of MDS and 20 subjects with hemocytopenia but a negative diagnosis of MDS as a control group. Reverse transcription-quantitative PCR and western blot analyses were used to measure HIF-1α expression in cells from the two groups and apoptosis and cell cycle were also analyzed and compared between the groups using flow cytometry assays. BM-MSCs from both the control group and the MDS group exhibited a fibroblast-like morphology, had similar growth cycles and were difficult to passage stably. It was observed that BM-MSCs from the MDS group had significantly higher HIF-1α expression levels than the control group (P<0.05). Furthermore, the BM-MSCs from the MDS group had a higher proportion of cells in early apoptosis (5.22±1.34 vs. 2.04±0.08%; P<0.0001) and late apoptosis (3.38±0.43 vs. 1.23±0.11%; P<0.01) and exhibited cell cycle arrest. This may be a noteworthy aspect of the pathogenesis of MDS and may be related to high HIF-1α expression under a hypoxic state in the bone marrow microenvironment. Furthermore, the expression of HIF-1α in bone marrow tissue sections from patients with MDS in the International Prognostic Scoring System (IPSS) lower-risk group was higher than that from patients with MDS in the IPSS high-risk group. These results revealed the role of HIF-1α as a central pathobiology mediator of MDS and an effective therapeutic target for a broad spectrum of patients with MDS, particularly for patients in the lower-risk group.

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“…BMSCs usually express surface markers, including CD90, CD44, CD29, CD105, and SH3, but not CD45, CD31, and CD14. 17 We used flow cytometry to detect surface CD molecules in BMSCs, and showed that CD44 and CD90 were positive, while CD45 and CD31 were negative. We induced the well-growing 3 rd -generation BMSCs to differentiate into osteoblasts, and identified the characteristics of osteoblasts, such as type I collagen, alkaline phosphatase, and mineralized nodules.…”

Section: Discussionmentioning

confidence: 99%

Effects of Mg-Nd-Gd-Sr alloy on bone marrow mesenchymal stem cell function derived from SD rats

Xie

Liu

et al. 2022

Eur J Inflamm

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Introduction The aim of this study was to determine the effect of a new type of Mg-3Nd-1Gd-0.3Sr-0.2Zn-0.4Zr (abbreviated to Mg-Nd-Gd-Sr) alloy on bone marrow mesenchymal stem cell (BMSCs) function derived from SD rats. Methods BMSCs were first isolated and cultured in vitro using the whole bone marrow adherence method, and identified by BMSC surface biomarkers and osteogenic induction. The in vitro biological safety of the Mg-Nd-Gd-Sr alloy was studied by cytotoxicity and apoptosis experiments, and the in vitro biological functions were studied by cell adhesion and cell proliferation experiments. Results The results showed that high-purity BMSCs were isolated using the whole bone marrow adherence method. Flow cytometry showed high expression of CD44 and CD90, and low expression of CD31 and CD45 in the BMSCs. Osteogenic induction showed that the BMSCs differentiated into osteoblasts, and mineralized nodules were observed. The cytotoxicity of the Mg-Nd-Gd-Sr alloy to SD rat BMSCs was 0–1 grade, suggesting that the Mg-Nd-Gd-Sr alloy had no significant cytotoxic effect on SD rat BMSCs; compared with the control group, there was no significant cell apoptosis in any of the experimental groups ( p > 0.05). Cell adhesion experiments showed that the number of adherent cells increased with the duration of culture with the exception of the 100% concentration group; compared with the control group, the 75% concentration group had the highest number of adherent cells at the 1st, 3rd, 5th, and 7th hours ( p < 0.05). Cell proliferation experiments showed that the number of cells in all experiment groups was higher than the control group ( p < 0.05) on the 1st, 3rd, 5th, and 7th days, with the highest number of cells in the 75% concentration group ( p < 0.05). Conclusion Our data indicate that the extracts of new type of Mg-Nd-Gd-Sr alloy has no apparent cytotoxicity to BMSCs, does not affect cell apoptosis, and has good biocompatibility. Different concentrations of Mg-Nd-Gd-Sr alloy extracts promoted the adhesion and proliferation of BMSCs. The alloy had good biological functions, and is thus a promising bone repair material.

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Comparative characteristic study from bone marrow-derived mesenchymal stem cells

Cited by 32 publications

References 107 publications

Osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cells

Osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cells

Relationship of HIF‑1α expression with apoptosis and cell cycle in bone marrow mesenchymal stem cells from patients with myelodysplastic syndrome

Effects of Mg-Nd-Gd-Sr alloy on bone marrow mesenchymal stem cell function derived from SD rats

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