Comparative characterization of growth and recombinant protein production among three insect cell lines with four kinds of serum free media

Kwon, Min Serk; Dojima, Takashi; Park, Enoch Y.

doi:10.1007/bf02940270

Cited by 19 publications

(11 citation statements)

References 5 publications

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“…However, the use of FBS is problematic because of its high cost, nonreproducibility due to lot-to-lot variation, undefined composition, increased contamination risk from mycoplasma and prion, downstream processing problems caused by its high protein concentration, and difficulties of obtaining FDA approval for pharmaceuticals produced in FBS medium. Therefore, many attempts have been made to develop serum-free media (Kwon et al, 2003).…”

Section: Resultsmentioning

confidence: 99%

Beneficial effect of silkworm hemolymph on a CHO cell system: Inhibition of apoptosis and increase of EPO production

Choi

Rhee

Park

2005

Biotech & Bioengineering

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To produce erythropoietin (EPO), Chinese hamster ovary (CHO) cells were first cultured in a medium containing FBS (growth medium) and then in a serum-free medium containing sodium butyrate (production medium). Sodium butyrate increases recombinant protein production, but also induces apoptosis, which reduces cell viability and productivity. In a previous study, we found that silkworm hemolymph (SH), an insect serum, inhibits the apoptosis of insect and mammalian cells. To overcome sodium butyrate-induced apoptosis, we added SH to growth medium. This pretreatment with SH inhibited the sodium butyrate-induced apoptosis of CHO cells and consequently increased their longevity and their ability to produce EPO. As a result, the volumetric productivity of EPO was increased five-fold. SH was found to inhibit cytochrome c release from mitochondria into the cytosol, and prevented the activation of caspase-3 and other subsequent caspase reactions.

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Section: Resultsmentioning

confidence: 99%

Beneficial effect of silkworm hemolymph on a CHO cell system: Inhibition of apoptosis and increase of EPO production

Choi

Rhee

Park

2005

Biotech & Bioengineering

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“…However, the application of each medium is restricted to cell types because of their different adaptabilities to various serum-free media. Kwon et al (2003) showed that serum-free Sf-900 II medium was suitable for Sf-9 and Sf-21 cells, whereas serum-free Express Five medium was preferable for Tn5B1-4 cells. In this paper, QB-Tn9-4s cells were trained to survive in both serum-free EX-CELL 420 and Sf-900 III media.…”

Section: Discussionmentioning

confidence: 98%

“…Inlow et al (1989) showed that Sf-9 had a shorter population doubling time in a serum-free suspension culture than in a serum-containing culture. Kwon et al (2003) compared the growth and protein expression of Sf-9, Sf-21, and BTITn5B1-4 in four different serum-free media and found that both Sf-9 and BTI-Tn5B1-4 cells possessed advantages and disadvantages in actual application. BTI-Tn5B1-4 cells are highly susceptible to baculovirus and could provide superior production of occlusion bodies (OBs) and recombinant proteins when compared to other insect cell lines.…”

Section: Introductionmentioning

confidence: 99%

Serum-Free Culture of the Suspension Cell Line QB-Tn9-4s of the Cabbage Looper,Trichoplusia ni, is Highly Productive for Virus Replication and Recombinant Protein Expression

Zheng¹,

Zhou²,

Li³

2014

Journal of Insect Science

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“…Mean specific consumption rates of glucose in Sf-900 II and Sf-900 III were 4.3 9 10 -12 and 3.1 9 10 -12 g cell -1 h -1 , respectively. Although specific rates of glucose consumption reported for insect cell lines vary under differing culture medium and cultivation conditions, one study has estimated the specific glucose consumption rates in suspension cultures of lepidopteran cell lines Sf-9, Sf-21 and BTI-Tn-5B1-4 at 3.7 9 10 -12 , 5.1 9 10 -12 and 14.5 9 10 -12 g cell -1 h -1 , respectively (Kwon et al 2003). BTI-Tn-5B1-4 is one of the highest yielding cell lines (up to 8 9 10 6 cells/ml in fed-batch culture) and correspondingly has a high specific rate of glucose consumption (Schmid 1996).…”

Section: Metabolites Analysesmentioning

confidence: 99%

Characterization of growth and Oryctes rhinoceros nudivirus production in attached cultures of the DSIR-HA-1179 coleopteran insect cell line

et al. 2013

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The DSIR-HA-1179 coleopteran cell line is a susceptible and permissive host to the Oryctes rhinoceros nudivirus (OrNV), which has been used as a biocontrol agent against the coconut rhinoceros beetle (Oryctes rhinoceros); a pest of palms in the Asia-Pacific region. However, little is known about growth and metabolism of this cell line, knowledge of which is necessary to develop an in vitro large-scale OrNV production process. The strong anchoragedependent characteristics of the cell line, its particular fragility and its tendency to form dense clumps when manipulated, are the most likely reasons that have precluded further development of the cell line. In order to characterize DSIR-HA-1179 cells, there was first a need for a reliable technique to count the cells. A homogenous cell suspension suitable for enumeration could be produced by treatment with TrypLE Express TM with optimum mean time for cell release calculated as 30 min. The cell line was adapted to grow in four serum-supplemented culture media namely TC-100, IPL-41, Sf-900 II and Sf-900 III and cell growth, glucose consumption, lactate and ammonia production were assessed from static-batch cultures. The maximum viable cell density was reached in Sf-900 II (17.9 9 10 5 cells/ml), with the maximum specific growth rate observed in this culture medium as well (0.0074 h -1 ). Higher production of OrNV was observed in IPL-41 and TC-100 (4.1 9 10 7 TCID 50 /ml) than in cultures infected in Sf-900 III (2.0 9 10 7 TCID 50 /ml) and Sf-900 II (1.4 9 10 7 TCID 50 /ml). At the end of the growth period, glucose was completely consumed in cultures grown in TC-100, while remained in excess in the other three culture media. The cell line produced lactate and ammonia to very low levels in the TC-100 culture medium which is a promising aspect for its cultivation at large-scale.

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Comparative characterization of growth and recombinant protein production among three insect cell lines with four kinds of serum free media

Cited by 19 publications

References 5 publications

Beneficial effect of silkworm hemolymph on a CHO cell system: Inhibition of apoptosis and increase of EPO production

Beneficial effect of silkworm hemolymph on a CHO cell system: Inhibition of apoptosis and increase of EPO production

Serum-Free Culture of the Suspension Cell Line QB-Tn9-4s of the Cabbage Looper,Trichoplusia ni, is Highly Productive for Virus Replication and Recombinant Protein Expression

Characterization of growth and Oryctes rhinoceros nudivirus production in attached cultures of the DSIR-HA-1179 coleopteran insect cell line

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