Comparative community structure of archaea in rumen of buffaloes and cattle

Paul, Souvik; Dey, Avijit; Baro, Daoharu; Punia, B.S.

doi:10.1002/jsfa.8177

Cited by 17 publications

(16 citation statements)

References 41 publications

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“…These ndings rea rm the previous reports on the dominance of Methanobrevibacter methanogens in cattle [39,40] and buffaloes [41,42]. A meta-analysis of archaeal 16S rRNA sequences available in GenBank [43] supports our results for the similar distribution of Methanobrevibacter in cattle and buffaloes. The higher abundance of Mbr.…”

Section: Discussionsupporting

confidence: 92%

“…Our results indicated that in spite of the small representation of Methanomassiliicoccales in the rumen archaeal community, they were more diversi ed in Indian ruminants than that of the New Zealand. A meta-analysis [43] strengthened our results on the higher abundance of Methanomassiliicoccales in cattle as compared to buffaloes. Though the methane producing ability of the Methanomassiliicoccales members has not yet been explored; their high relative abundance, i.e., up to 50% of the total rumen archaea [24,57], is a clear indication of their possible involvement in rumen methanogenesis.…”

Section: Discussionsupporting

confidence: 77%

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Influence of Host and Geographical Regions on the Rumen Methanogens Diversity in Indian Cattle and Buffaloes

Trivedi¹,

Malik²,

Kolte³

et al. 2020

Preprint

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Background Archaea belong to the domain Euryarchaeota, represents only 3-5% of the rumen microbiota; however, they play a central role in maintaining the H2 pressure. Host species in conjunction with geographical regions may lead to the variable distribution of rumen methanogens. Indian cattle and buffaloes represent 13 and 53% of the global population, respectively. However, the influence of host species (bovine) and geographical regions on the methanogens distribution has not been investigated in Indian subcontinent. Therefore, the present study was undertaken to explore the rumen methanogens diversity in the cattle and buffaloes native to the two distinct geographical regions of India. Results Next generation sequencing of 16S rRNA gene from 36 rumen fluid samples corresponding to cattle and buffaloes revealed that Methanobrevibacter (Mbr.) genus constituted largest fraction (55-62%) of the rumen archaeal community. Furthermore, Methanobrevibacter gottschalkii was the most prominent methanogens with the significantly higher abundance in cattle. Methanomassiliicoccales represented <10% of the total archaeal community and was found to be the most variable between these two host species. In north region, the distribution of Methanomassiliicoccales, such as Methanosphaera sp., Group 4, Group 8, Group 12 sp. and Group 9 sp. was significantly higher in cattle. The distribution of prominent methanogens, such as Mbr. gottschalkii and Mbr. ruminantium did not differ between two host species in the north region. However, Mbr. gottschalkii was significantly higher in buffaloes as compared to cattle in south region. Mbr. wolinii was exclusively detected in the cattle. Inter-region comparison established that Methanomassiliicoccales were the most variable between the two regions. Conclusions Host species did not affect the distribution of hydrogenotrophic methanogens except for Mbr. gottschalkii. Methylotrophic methanogens, in spite of their limited representation, were most influenced by the host species and geographical regions. Overall, the Methanomassiliicoccales distribution was higher in cattle, and the environmental conditions in north region were conducive for their higher distribution. The variable distribution of methanogens indicated that host and geographical region oriented strategies needs to be developed for the reduction of enteric methane emission. Further studies are warranted to explore the impact of diet on the distribution of rumen methanogens between the host species within and across different environmental conditions.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 77%

Influence of Host and Geographical Regions on the Rumen Methanogens Diversity in Indian Cattle and Buffaloes

Trivedi¹,

Malik²,

Kolte³

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…Using a rumen-derived inoculum, which harbours high microbial hydrolytic activities, could reduce the pre-treatment costs in anaerobic digestion since there is little or no requirement to add hydrolytic enzymes [ 18 ]. The rumen contains a large number of microorganisms with enormous diversity, of which includes at least 50 bacterial genera (10 10 –10 11 cells ml −1 ), 25 genera of ciliate protozoa (10 4 –10 6 cells ml −1 ), 6 genera of fungi (10 3 –10 5 zoospores ml −1 ), 11 genera of methanogenic archaea (10 9 cells ml −1 ) and bacteriophages (10 8 –10 9 phages ml −1 ) [ 19 , 20 ]. Rumen microorganisms are naturally involved in the degradation of carbohydrates and lignocellulosic biomass to a variety of VFAs and OAs [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Organic acid production from potato starch waste fermentation by rumen microbial communities from Dutch and Thai dairy cows

Ayudthaya

Weijer

Gelder

et al. 2018

Biotechnol Biofuels

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BackgroundExploring different microbial sources for biotechnological production of organic acids is important. Dutch and Thai cow rumen samples were used as inocula to produce organic acid from starch waste in anaerobic reactors. Organic acid production profiles were determined and microbial communities were compared using 16S ribosomal ribonucleic acid gene amplicon pyrosequencing.ResultsIn both reactors, lactate was the main initial product and was associated with growth of Streptococcus spp. (86% average relative abundance). Subsequently, lactate served as a substrate for secondary fermentations. In the reactor inoculated with rumen fluid from the Dutch cow, the relative abundance of Bacillus and Streptococcus increased from the start, and lactate, acetate, formate and ethanol were produced. From day 1.33 to 2, lactate and acetate were degraded, resulting in butyrate production. Butyrate production coincided with a decrease in relative abundance of Streptococcus spp. and increased relative abundances of bacteria of other groups, including Parabacteroides, Sporanaerobacter, Helicobacteraceae, Peptostreptococcaceae and Porphyromonadaceae. In the reactor with the Thai cow inoculum, Streptococcus spp. also increased from the start. When lactate was consumed, acetate, propionate and butyrate were produced (day 3–4). After day 3, bacteria belonging to five dominant groups, Bacteroides, Pseudoramibacter_Eubacterium, Dysgonomonas, Enterobacteriaceae and Porphyromonadaceae, were detected and these showed significant positive correlations with acetate, propionate and butyrate levels.ConclusionsThe complexity of rumen microorganisms with high adaptation capacity makes rumen fluid a suitable source to convert organic waste into valuable products without the addition of hydrolytic enzymes. Starch waste is a source for organic acid production, especially lactate.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1012-4) contains supplementary material, which is available to authorized users.

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“…The rumen contains a large number of microorganisms with enormous diversity. At least 50 bacterial genera (10 10 -10 11 cells ml -1 ), 25 genera of ciliate protozoa (10 4 -10 6 cells ml -1 ), 6 genera of fungi (10 3 -10 5 zoospores ml -1 ), 11 genera of methanogenic archaea (10 9 cells ml -1 ) and bacteriophages (10 8 -10 9 phages ml -1 ) are present in the rumen of ruminants (Cobellis et al, 2016;Paul et al, 2017). The microbial diversity composition also varies depending on the type of host and the environmental conditions, such as domestic or wild animals and their diets .…”

Section: Animal Gut or Rumen Microorganisms And Organic Acid Productionmentioning

confidence: 99%

“…Using a rumen-derived inoculum, which harbours high microbial hydrolytic activities, could reduce the pre-treatment costs in anaerobic digestion since there is little or no requirement to add hydrolytic enzymes (Thoetkiattikul et al, 2013). The rumen contains a large number of microorganisms with enormous diversity, of which includes at least 50 bacterial genera (10 10 -10 11 cells ml -1 ), 25 genera of ciliate protozoa (10 4 -10 6 cells ml -1 ), 6 genera of fungi (10 3 -10 5 zoospores ml -1 ), 11 genera of methanogenic archaea (10 9 cells ml -1 ) and bacteriophages (10 8 -10 9 phages ml -1 ) (Cobellis et al, 2016;Paul et al, 2017). Rumen microorganisms are naturally involved in the degradation of carbohydrates and lignocellulosic biomass to a variety of volatile fatty acids and organic acids (Yue et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Organic acid production from starchy waste by gut derived microorganisms

Ayudthaya¹

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Parameter Chemical routes Non-renewable feedstockspetrochemicals Fermentative routes Biobased feedstockcarbohydrates Price considerations Still cheaper than the renewable sources. Feedstock themselves do not contribute to the price as much as downstream processing Availability Availability expected to decrease in time Abundant and renewable Routes Developed routes, established technologies Routes under constant improvement, young technologies Yields and productivities Generally high Sometimes a large number of side products, diluted media, long reaction times Major disadvantages High energy demands (pressure and temperature). Catalysts disposal issues Sensitivity of microorganisms, nutrient requirements, complicated product recovery, large amounts of waste Environment effect Release CO2 that contributes to global warming Consume CO2 via TCA cycle Public awareness Decreasing popularity Increased interest in improving currently applied routes and innovations Current high costs of production prevent bio-based chemicals to be widely used. Thus, production processes from bio-based resources need to be developed and optimized. It is estimated that in 2050 approximately 30% (by weight) of chemicals will be obtained from renewable biomass (http://www.suschem.org) (Fiorentino and Ripa, 2017). Biomass is an abundant carbon-neutral renewable resource that can be used as a carbon source instead of fossil feedstocks (Fiorentino and Ripa, 2017). Production chains resulting from biomass are considered as "short-cycle carbon systems", which are more sustainable and preferable than those resulting from fossil resources, which are considered as "long-cycle carbon systems" (Kajaste et al., 2014; Fiorentino and Ripa, 2017). The transition from fossil based to bio-based economy needs the development of innovative processes to exploit the potential of biomass (Pleissner et al., 2017). Thus, utilization of food wastes or biomass and their conversion into valuable products require more attention as the implementation of such system is not yet successfully guaranteed. However, many criteria need to be considered for * Guyot et al. (2000) * Narita et al. 2004 Porphyromonadaceae. In the reactor with the Thai cow inoculum, Streptococcus spp. also increased from the start. When lactate was consumed, acetate, propionate and butyrate were produced (day 3-4). After day 3, bacteria belonging to five dominant groups, Bacteroides, Pseudoramibacter_Eubacterium, Dysgonomonas, Enterobacteriaceae and Porphyromonadaceae were detected and these showed significant positive correlations with acetate, propionate and butyrate levels. The complexity of rumen microorganisms with high adaptation capacity makes rumen fluid a suitable source to convert organic waste into valuable products without the addition of hydrolytic enzymes. Starch waste is a source for organic acid production, especially lactate.

show abstract

Comparative community structure of archaea in rumen of buffaloes and cattle

Cited by 17 publications

References 41 publications

Influence of Host and Geographical Regions on the Rumen Methanogens Diversity in Indian Cattle and Buffaloes

Influence of Host and Geographical Regions on the Rumen Methanogens Diversity in Indian Cattle and Buffaloes

Organic acid production from potato starch waste fermentation by rumen microbial communities from Dutch and Thai dairy cows

Organic acid production from starchy waste by gut derived microorganisms

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