Comparative electrophoretic study on ribosomal proteins from algae

Abstract

The proteins from cytoplasmic ribosomal subunits of eight species of algae were analyzed by two-dimensional gel electrophoresis. The molecular weights of the proteins were in the range of 10,000 to 55,000. We have compared the protein patterns from the ribosomal subunits of the different species to those of Chlamydomonas reinhardii. It was quite clear that there are many similarities in the protein patterns of all the investigated species. We found for Chlamydomonas eugametos 48, Chlamydomonas noctigama 42, Chlorogonium elongatum 47, Scenedesmus obliquus 40, Chlorella fusca 35, and Euglena gracilis 35 proteins which were homologous to those of Chlamydomonas reinhardii. For the colorless flagellate Polytoma papillatum, we detected 45 proteins homologous to Chlamydomonas reinhardii, so that the generally assumed close relationship between Chlamydomonas and Polytoma is confirmed.

Proteomics in Seaweeds: Ecological Interpretations

“…Their results showed that the number of proteins in both small and large subunits was higher in cytoplasmic than in chloroplastic ribosomes, indicating that cytoplasmic ribosomes are more complex. Another study did a comparison between the ribosomal proteins of 8 species including C. reinhardtii (Götz & Arnold, 1980). The results showed that the number of proteins in both subunits was similar among all species, and that the Polytoma papillatun (Plantae, Chlorophyta) proteins were the most similar to those of C. reinhardtii in terms of protein homology.…”

“…Götz & Arnold, 1980;Hanson et al, 1974). The Hanson et al (1974) work based their protocols on the Mets & Bogorad (1972) research paper and also used C. reinhardtii as model species.…”

“…Regardless of this, some spots were easily detected in the gels allowing for comparison between wild-types and mutant strains. Finally, in the work of Götz & Arnold (1980) ribosomal proteins from eight species were evaluated with two gels showing clear and well-resolved 2-DE patterns. The protein extraction was well suited for all species.…”

Plant cytoplasmic ribosomal proteins: an update on classification, nomenclature, evolution and resources

“…Plant RPs were first reported in 1960 (Setterfield et al., 1960). For much of the next 20 years, the principal focus was the isolation and characterization of plant RPs by using electrophoretic techniques; the proteins were numbered using integers as a function of their positions on gels (Götz & Arnold, 1980; Hanson et al., 1974; Nagabhushan et al., 1974; Vasconcelos & Bogorad, 1971). As different labs numbered the same protein with a different number, there was a strong need to develop a systematic nomenclature for RPs.…”