Comparative evaluation of nonradiometric BACTEC and improved oxoid signal blood culture systems in a clinical laboratory

Daley, Charles L.; Lim, Irene; Modra, J; Wilkinson, Irene J

doi:10.1128/jcm.28.7.1586-1590.1990

Cited by 22 publications

(4 citation statements)

References 8 publications

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“…The differential white cell count was performed by microscopic examination at 400 and 1000 magnification, counting 100 cells. A sample for blood culture was collected aseptically and a commercial culture system (OXOID SIGNAL Blood Culture System, Oxoid) was used as previously reported (Daley et al, 1990;Rohner et al, 1995). The outcome was recorded for all the sick client-owned calves as 'discharged' or 'died/euthanased'.…”

Section: Collection Of Clinical and Clinical Pathological Datamentioning

confidence: 99%

Plasma procalcitonin concentration in healthy calves and those with septic systemic inflammatory response syndrome

Bonelli¹,

Meucci²,

Divers

et al. 2018

The Veterinary Journal

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The diagnosis of sepsis in calves is challenging. Blood culture and clinical signs combined with a complete blood count have been used for the diagnosis of sepsis. Recent literature in humans and animal species has been focused on sepsis-specific biomarkers, such as procalcitonin (PCT), that may more accurately and efficiently diagnose sepsis. The aim of this study was to evaluate plasma PCT concentrations in healthy and septic calves. Twenty healthy control calves and 58 sick calves with septic systemic inflammatory response syndrome (SIRS) based on SIRS score and clinical findings were included. Calves with septic SIRS were further divided in septic SIRS survivors (SSS) and non-survivors (SSNS). Plasma PCT concentrations were measured with a commercial ELISA assay for cattle. A receiver operating characteristic curve was used to determine cut-off values and corresponding sensitivity and specificity for the diagnosis of sepsis. Differences in plasma PCT concentration between groups (control vs. SSS vs. SSNS) were evaluated. Plasma PCT concentrations in healthy calves and those with septic SIRS were 33.3pg/mL (0-44.3pg/mL) and 166.5pg/mL (85.9-233.0pg/mL), respectively (P<0.001). The optimal cut-off value to predict septic SIRS was 67.39pg/mL (81.0% sensitivity, 95.0% specificity). Plasma PCT concentrations were 127.4pg/mL (72.2-216.0pg/mL) and 234.3pg/mL (204.5-309.4pg/mL) in the SSS and SSNS subgroups, respectively. Statistically significant differences were found among groups (control vs. SSS and SSNS, P<0.0001; SSS vs. SSNS, P>0.05). These results confirmed an increase in plasma PCT concentrations in calves with septic SIRS, as previously reported in humans and other species.

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Section: Collection Of Clinical and Clinical Pathological Datamentioning

confidence: 99%

Plasma procalcitonin concentration in healthy calves and those with septic systemic inflammatory response syndrome

Bonelli¹,

Meucci²,

Divers

et al. 2018

The Veterinary Journal

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“…The results obtained during the first evaluations of the Signal system were inferior ( P <0.0001) to those of BACTEC 6B and 7D combined media [56]. Modification of the bottle headspace atmosphere and agitation of the system during the first 24–48 h of incubation improved the performance of the system [53, 57–59] (Table 2). In spite of these improvements, the single‐bottle Signal system detected less pathogens than systems consisting of two media (aerobic and anaerobic) from the same volume of blood, i.e.…”

Section: Manual Systemsmentioning

confidence: 99%

Review on evaluations of currently available blood-culture systems

Rohner

Auckenthaler

1999

Clinical Microbiology and Infection

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“…The performance of the Signal manometric blood culture system (Unipath) has already been compared with a variety of conventional and automated methods (Murray et al 1988;Weinstein et al 1988Weinstein et al , 1989Wilson et al 1988;Rohner and Auckenthaler 1989). Three comparisons of the Signal with the Bactec non-radiometric NR-660 system (Becton Dickinson) have been reported (Rimmer and Cabot 1988;Daley et al 1990;Schwabe et al 1990). In the study by Rimmer and Cabot (1988), the Signal system was found to be inferior.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Signal and Bactec NR‐660 blood culture systems

Stevens

Prentice

Swann

et al. 1993

Journal of Applied Bacteriology

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The Signal blood culture system was compared with the Bactec NR-660. A total of 1617 blood culture sets yielded 143 (8.8%) significant isolates; 113 (79.0%) were from positive bottles in both the Bactec and Signal systems. Twelve organisms (8.4%) were detected and isolated from the Signal system only and another 18 (12.6%) from the Bactec system only. Of these 18, five were Signal-positive but the organism was not recovered and four organisms were isolated from negative Signal bottles on terminal subculture. The time taken to detection for each system was similar; the Signal system detected 68% and the Bactec 63% of significant positives within 24 h. At 48 h Bactec detected 91% and the Signal 85%. A significantly-reduced number of bottles which gave a positive signal but were negative by microscopical and cultural methods was found, compared with previous reports. The 1 h incubation period prior to the insertion of the Signal growth indicator device was considered to be the cause of this reduction in the proportion of false positives. Fifty-five percent (42/77) of the Bactec false positives were due to delta growth value. This is when there is an increase in the growth index of > or = 15 without the positive threshold level of 30 being attained. This occurred in the anaerobic bottle on day 2 with 42 bottles. Another 40% (31/77) of the false positives had a growth value between the positive threshold of 30 and a value of 35. Eighty (4.9%) of Bactec and 65 (4.0%) of Signal sets yielded clinically non-significant isolates.(ABSTRACT TRUNCATED AT 250 WORDS)

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Comparative evaluation of nonradiometric BACTEC and improved oxoid signal blood culture systems in a clinical laboratory

Cited by 22 publications

References 8 publications

Plasma procalcitonin concentration in healthy calves and those with septic systemic inflammatory response syndrome

Plasma procalcitonin concentration in healthy calves and those with septic systemic inflammatory response syndrome

Review on evaluations of currently available blood-culture systems

Comparison of Signal and Bactec NR‐660 blood culture systems

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