Comparative fermentability of enzymatic and acid hydrolysates of steam-pretreated aspenwood hemicellulose by Pichia stipitis CBS 5776

Wilson, J. Jeffrey; Deschatelets, Lise; Nishikawa, Nora K.

doi:10.1007/bf00270801

Cited by 91 publications

(37 citation statements)

References 17 publications

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“…Vacuum evaporation is capable of reducing volatile compounds such as furfural, acetic acid and vanillin [37]. Solvents (e.g., ethyl acetate) and active charcoal and/or ion-exchange resins reduce the concentration of inhibitors by extraction or adsorption, respectively [38][39][40]. Chemical transformation of inhibitors is also possible by addition of reducing agents (dithionite and sulfite) [41] and chemical catalysts, being overliming (treatment with Ca(OH) 2 ) the most efficient chemical detoxification method for removing phenols and furan derivatives [37].…”

Section: Detoxification Of Pretreated Materialsmentioning

confidence: 99%

Laccases as a Potential Tool for the Efficient Conversion of Lignocellulosic Biomass: A Review

et al. 2017

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Abstract:The continuous increase in the world energy and chemicals demand requires the development of sustainable alternatives to non-renewable sources of energy. Biomass facilities and biorefineries represent interesting options to gradually replace the present industry based on fossil fuels. Lignocellulose is the most promising feedstock to be used in biorefineries. From a sugar platform perspective, a wide range of fuels and chemicals can be obtained via microbial fermentation processes, being ethanol the most significant lignocellulose-derived fuel. Before fermentation, lignocellulose must be pretreated to overcome its inherent recalcitrant structure and obtain the fermentable sugars. Usually, harsh conditions are required for pretreatment of lignocellulose, producing biomass degradation and releasing different compounds that are inhibitors of the hydrolytic enzymes and fermenting microorganisms. Moreover, the lignin polymer that remains in pretreated materials also affects biomass conversion by limiting the enzymatic hydrolysis. The use of laccases has been considered as a very powerful tool for delignification and detoxification of pretreated lignocellulosic materials, boosting subsequent saccharification and fermentation processes. This review compiles the latest studies about the application of laccases as useful and environmentally friendly delignification and detoxification technology, highlighting the main challenges and possible ways to make possible the integration of these enzymes in future lignocellulose-based industries.

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Section: Detoxification Of Pretreated Materialsmentioning

confidence: 99%

Laccases as a Potential Tool for the Efficient Conversion of Lignocellulosic Biomass: A Review

et al. 2017

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“…Although the optimization conditions for maximum sugar recoveries can be predicted, the nature of the inhibitory compounds and methods to detoxify the streams in a costeffective manner are still largely unresolved. Traditional detoxification methods, such as the addition of activated coal (40), extraction with organic solvents (41)(42)(43), ion-exchange (41,42,44), ion exclusion (45), molecular sieves (46), overliming (47,48), and steam stripping (49), have been shown to be costly or ineffective (50). Although there have been some attempts to adapt the fermentation organisms to the inhibitory substances (51), each feedstock change required a further adaptation period.…”

Section: Hemicellulose Recoverymentioning

confidence: 99%

A Techno-Economic Assessment of the Pretreatment and Fractionation Steps of a Biomass-to-Ethanol Process

Gregg

Saddler

1996

Seventeenth Symposium on Biotechnology for Fuels and Chemicals

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It is generally recognized that the front-end (pretreatment, fractionation, enzymatic hydrolysis) steps of a lignocellulose-to-ethanol process are both technologically immature and represent a large component (-60%) of the total product cost. In the past, we have tried to itemize the process steps and equipment for a complete plant. It was evident that, owing to the complexity and interrelated nature of this process, it was difficult to determine the influence of even minor changes to the process on the overall production cost of the product. We had originally developed a techno-economic model, based on spreadsheets, as a computational and assessment tool. However, our more recent work, which has looked at various process options such as hardwood vs softwoods, 502 pretreatment of softwoods, and enzyme recycling, indicated that the model required greater flexibility if it was to assess a "generic" biomass-to-ethanol process. The model is currently being modified to address both the flexibility issues, through the incorporation of flowsheeting concepts, as well as including the most recent work on the various process options. In this article, we have described some of the pretreatment and fractionation issues that are being addressed in the updated model.

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“…Palmqvist et al, [1996] observed the removal of most volatile fraction (10% v/v) from willow hemicellulose hydrolysate by roto-evaporation. Wilson et al, [1989] found a decrease in the concentration of acetic acid, furfural and vanillin by 54%, 100% and 29%, respectively, compared with the concentrations in the hydrolysate. Larsson et al, [1999] observed the removal of furfural (90%) and HMF (4%) using vacuum evaporation from wood hemicellulosic hydrolysate.…”

Section: Physical Methods 411 Evaporationmentioning

confidence: 84%

“…The extraction of fermentation inhibitors using ethyl acetate has been found to increase the ethanol yield in fermentation by P. stipitis from 0 to 93% of that obtained in the reference fermentation [Wilson et al, 1989]. The extraction procedure could eliminate acetic acid (56%), and total furfural, vanillin, and 4-hydroxybenzoic acid.…”

Section: Extraction With Ethyl Acetatementioning

confidence: 98%

Detoxification of Lignocellulosic Hydrolysates for Improved Bioethanol Production

Chandel¹,

Silva²,

Singh³

2011

Biofuel Production-Recent Developments and Prospects

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Comparative fermentability of enzymatic and acid hydrolysates of steam-pretreated aspenwood hemicellulose by Pichia stipitis CBS 5776

Cited by 91 publications

References 17 publications

Laccases as a Potential Tool for the Efficient Conversion of Lignocellulosic Biomass: A Review

Laccases as a Potential Tool for the Efficient Conversion of Lignocellulosic Biomass: A Review

A Techno-Economic Assessment of the Pretreatment and Fractionation Steps of a Biomass-to-Ethanol Process

Detoxification of Lignocellulosic Hydrolysates for Improved Bioethanol Production

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