Comparative high-performance liquid chromatographic and high-performance thin-layer chromatographic study for the simultaneous determination of dapagliflozin and metformin hydrochloride in bulk and pharmaceutical formulation

Simple TLC–spectrodensitometric method for studying lipophilicity and quantitative analysis of hypoglycemic drugs in their binary mixture

“…It was found that the recovery percentages were in the range of 98.4–100.4%. The reported methods were used for comparison with the results obtained (Nasser et al, 2018), as shown in Table 2. Comparisons using t‐ and F ‐tests at 95% confidence level were applied, which prove that the difference between the proposed and the reported methods is not significant .…”

“…As a result, they are used in combination therapy with other antidiabetic drugs such as MET (Lajara, 2014). The reported methods for analysis of DAPA and MET in combination include UV spectrophotometry (Bhavyasri & Surekha, 2020; Jani et al, 2015), RP‐HPLC (Abbas et al, 2020; Khalil et al, 2018; Urooj et al, 2017), LC–UV (Hassib et al, 2019), LC–MS/MS (Dias et al, 2019; Shah et al, 2019), capillary electrophoresis (Maher et al, 2019) and TLC–spectrodensitometric methods for simultaneous analysis of DAPA and MET (Abdelrahman et al, 2020; Nasser et al, 2018). These methods are less sensitive with several mobile phases and are applied only to pharmaceutical formulations.…”

A New High Performance Thin Layer Chromatographic Method Development and Validation of Dapagliflozin in Bulk and Tablet Dosage Form

“…Many spectrophotometric methods [2][3] and chromatographic methods [4][5][6][7][8][9][10][11][12][13] have been reported for estimation of DAPA in bulk and formulation. In combination, DAPA has to be analysed by chromatographic methods [14,15]. A work carried out by Sally et al [14] used acetonitrile: ammonium acetate:10% acetic acid (9:0.9:0.1, v/v) as the mobile phase at 225 nm.…”

“…In combination, DAPA has to be analysed by chromatographic methods [14,15]. A work carried out by Sally et al [14] used acetonitrile: ammonium acetate:10% acetic acid (9:0.9:0.1, v/v) as the mobile phase at 225 nm. In another study reported by Parmar et al [15] carried out for simultaneous estimation of DAPA, propanediol and saxagliptin Hydrochloride (SAXA) using Chloroform: Ethyl acetate: methanol: ammonia (6:2:2:2 Drops) as Mobile Phase and 210 nm as detector wave length, the Rf value of DAPA and SAXA was found to be 0.30 and 0.54 respectively.…”

“…However, few methods were developed for the combination of drugs. A comparative evaluation of HPLC and HPTLC estimation was carried out for simultaneous determination of dapagliflozin and metformin hydrochloride [14] and UV-spectroscopic first derivative and HPTLC methods were done to estimate dapagliflozin propanediol monohydrate and saxagliptin hydrochloride [15]. This necessitates the requirement for the development of newer methods of analysis for DAPA so that it can be used in routine analysis.…”