Comparative Metabolomic and Proteomic Analyses Reveal the Regulation Mechanism Underlying MeJA-Induced Bioactive Compound Accumulation in Cutleaf Groundcherry (Physalis angulata L.) Hairy Roots

Abstract

Cutleaf groundcherry ( Physalis angulata L.) is an annual plant with a number of medicinal ingredients. However, studies about the secondary metabolism of P. angulata are very limited. An integrated metabolome and proteome approach was used to reveal the variations in the metabolism associated with bioactive compounds under methyl-jasmonate (MeJA) treatment. Application of MeJA to the hairy roots could significantly increase the accumulation of most active ingredients. A targeted approach confirmed the variations in physalins D and H between MeJA treatment and the controls. Increases in the levels of a number of terpenoid backbone biosynthesis and steroid biosynthesis related enzymes, cytochrome P450 monooxygenases and 3β-hydroxysterioid dehydrogenase might provide a potential explanation for the MeJA-induced active ingredient synthesis. Our results may contribute to a deeper understanding of the regulation mechanism underlying the MeJA-induced active compound accumulation in P. angulata.

Transcriptomic Landscape of Medicinal Dendrobium Reveals Genes Associated With the Biosynthesis of Bioactive Components

“…For example, the alkaloid biosynthesis was markedly increased in Catharanthus roseus and Motherwort by binary stress and drought stress, respectively (Wei et al, 2013;Zhu et al, 2015). The phytohormone MeJA has been identified as a signaling molecule that switches on gene expression and enhances the biosynthesis of various bioactive compounds, particularly alkaloids and polysaccharides in medicinal plants including Dendrobium species (Zhan et al, 2018;Zhang et al, 2018). Exogenous feeding of MeJA enhanced the catalytic efficiency and the expression of strictosidine synthase (STR), which plays a vital role in alkaloid biosynthesis (Paul et al, 2017).…”

Comparative analysis of maca (Lepidium meyenii) proteome profiles reveals insights into response mechanisms of herbal plants to high-temperature stress

“…Furthermore, a gene ontology (GO) analysis of the SDEPs was also performed as described previously [20]. As shown in Fig.…”

“…To gain more information about the potential functions of these HTS-responsive SDEPs, a GO enrichment analysis was conducted as described previously [20]. As shown in Fig.…”

“…HPLC fractionation was conducted an Agilent 300Extend-C18 column (4.6 × 250 mm, 5 μm; Agilent Technologies, USA) as described by Zhan et al [20]. Briefly, the peptides were first separated with a gradient of 8 to 32% acetonitrile (pH 9.0) at a flow rate of 0.5 mL min − 1 over 60 min into 60 fractions.…”

“…Protein annotation, classification and enrichment analyses GO and domain annotations were conducted as described by Zhan et al [20]. In brief, GO annotation of the HTS-responsive proteome of maca was conducted by searching against the UniProt-GOA database (http:// www.ebi.ac.uk/GOA).…”